Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation
Demand for microbial amylase production persists because of its immense importance in wide spectrum industries. The present work has been initiated with a goal of optimization of solid state fermentation condition for amylase using agroindustrial waste and microbial strain like B. amyloliquefaciens...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/764046 |
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| author | Koel Saha Sujan Maity Sudeshna Roy Koustav Pahan Rishija Pathak Susmita Majumdar Suvroma Gupta |
| author_facet | Koel Saha Sujan Maity Sudeshna Roy Koustav Pahan Rishija Pathak Susmita Majumdar Suvroma Gupta |
| author_sort | Koel Saha |
| collection | DOAJ |
| description | Demand for microbial amylase production persists because of its immense importance in wide spectrum industries. The present work has been initiated with a goal of optimization of solid state fermentation condition for amylase using agroindustrial waste and microbial strain like B. amyloliquefaciens (MTCC 1270). In an aim to improve the productivity of amylase, fermentation has been carried out in the presence of calcium (Ca+2), Nitrate (NO3−), and chloride ions (Cl−) as well as in the presence of D-inositol and mannitol. Amylase needs calcium ion for the preservation of its structure, activity and stability that proves beneficial also for amylase production using solid state fermentation. The inclusion of ions and sugars in the SSF media is promising which can be explained by the protection offered by them against thermal decay of amylase at various incubation periods at 37°C. |
| format | Article |
| id | doaj-art-9aafd39edf564d7f9550ea14b80c594b |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-9aafd39edf564d7f9550ea14b80c594b2025-02-03T01:01:41ZengWileyInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/764046764046Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State FermentationKoel Saha0Sujan Maity1Sudeshna Roy2Koustav Pahan3Rishija Pathak4Susmita Majumdar5Suvroma Gupta6Department of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDepartment of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDepartment of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDepartment of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDepartment of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDepartment of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDepartment of Biotechnology, Haldia Institute of Technology, ICARE Complex, Purba Medinipur, West Bengal 721657, IndiaDemand for microbial amylase production persists because of its immense importance in wide spectrum industries. The present work has been initiated with a goal of optimization of solid state fermentation condition for amylase using agroindustrial waste and microbial strain like B. amyloliquefaciens (MTCC 1270). In an aim to improve the productivity of amylase, fermentation has been carried out in the presence of calcium (Ca+2), Nitrate (NO3−), and chloride ions (Cl−) as well as in the presence of D-inositol and mannitol. Amylase needs calcium ion for the preservation of its structure, activity and stability that proves beneficial also for amylase production using solid state fermentation. The inclusion of ions and sugars in the SSF media is promising which can be explained by the protection offered by them against thermal decay of amylase at various incubation periods at 37°C.http://dx.doi.org/10.1155/2014/764046 |
| spellingShingle | Koel Saha Sujan Maity Sudeshna Roy Koustav Pahan Rishija Pathak Susmita Majumdar Suvroma Gupta Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation International Journal of Microbiology |
| title | Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation |
| title_full | Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation |
| title_fullStr | Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation |
| title_full_unstemmed | Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation |
| title_short | Optimization of Amylase Production from B. amyloliquefaciens (MTCC 1270) Using Solid State Fermentation |
| title_sort | optimization of amylase production from b amyloliquefaciens mtcc 1270 using solid state fermentation |
| url | http://dx.doi.org/10.1155/2014/764046 |
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