Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components
Abstract The effects of low-intensity ultrasound on plants such as piezoelectric and ultrasonic water baths, on plants have been extensively studied. However, the specific effect of airborne ultrasound on plant cells has yet to be reported. The present study was conducted to elucidate the physiologi...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87762-6 |
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| author | Mahsa Sardari Faezeh Ghanati Hamid Mobasheri Abazar Hajnorouzi |
| author_facet | Mahsa Sardari Faezeh Ghanati Hamid Mobasheri Abazar Hajnorouzi |
| author_sort | Mahsa Sardari |
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| description | Abstract The effects of low-intensity ultrasound on plants such as piezoelectric and ultrasonic water baths, on plants have been extensively studied. However, the specific effect of airborne ultrasound on plant cells has yet to be reported. The present study was conducted to elucidate the physiological responses of plant cells to airborne US. Homogeneous suspension-cultured tobacco cells (Nicotiana tabacum L. cv Burley 21) were subjected to airborne US at 24 kHz in two pulsatile and continuous modes for 10 and 20 s. The study’s outcome revealed that airborne US triggered the production of H2O2, elevated internal calcium concentration, and reduced antioxidant capacity upon cavitation. Alteration of covalently bound peroxidase and other wall-modifying enzyme activities was accompanied by reduced cellulose, pectin, and hemicellulose B but increased lignin and hemicellulose A. The biomass and viability of tobacco cells were also significantly decreased by airborne US, which ultimately resulting in PCD and secondary necrosis. The results highlight the potential risks of even short-time exposure to the airborne US on plant physiology and cell wall chemical composition raising significant concerns about its implications. |
| format | Article |
| id | doaj-art-9aaf19c5c60840eea3a0776cbcd75e89 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-9aaf19c5c60840eea3a0776cbcd75e892025-02-02T12:17:01ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-025-87762-6Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall componentsMahsa Sardari0Faezeh Ghanati1Hamid Mobasheri2Abazar Hajnorouzi3Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares UniversityDepartment of Plant Biology, Faculty of Biological Science, Tarbiat Modares UniversityLaboratory of Membrane Biophysics and Macromolecules, Institute of Biochemistry and Biophysics, University of TehranDepartment of Physics, Faculty of Basic Sciences, Shahed UniversityAbstract The effects of low-intensity ultrasound on plants such as piezoelectric and ultrasonic water baths, on plants have been extensively studied. However, the specific effect of airborne ultrasound on plant cells has yet to be reported. The present study was conducted to elucidate the physiological responses of plant cells to airborne US. Homogeneous suspension-cultured tobacco cells (Nicotiana tabacum L. cv Burley 21) were subjected to airborne US at 24 kHz in two pulsatile and continuous modes for 10 and 20 s. The study’s outcome revealed that airborne US triggered the production of H2O2, elevated internal calcium concentration, and reduced antioxidant capacity upon cavitation. Alteration of covalently bound peroxidase and other wall-modifying enzyme activities was accompanied by reduced cellulose, pectin, and hemicellulose B but increased lignin and hemicellulose A. The biomass and viability of tobacco cells were also significantly decreased by airborne US, which ultimately resulting in PCD and secondary necrosis. The results highlight the potential risks of even short-time exposure to the airborne US on plant physiology and cell wall chemical composition raising significant concerns about its implications.https://doi.org/10.1038/s41598-025-87762-6Airborne ultrasoundCell wall componentsCell deathMembrane integrityRedox statusTobacco cells |
| spellingShingle | Mahsa Sardari Faezeh Ghanati Hamid Mobasheri Abazar Hajnorouzi Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components Scientific Reports Airborne ultrasound Cell wall components Cell death Membrane integrity Redox status Tobacco cells |
| title | Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components |
| title_full | Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components |
| title_fullStr | Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components |
| title_full_unstemmed | Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components |
| title_short | Short-term airborne ultrasound induced cell death in tobacco cells and changed their wall components |
| title_sort | short term airborne ultrasound induced cell death in tobacco cells and changed their wall components |
| topic | Airborne ultrasound Cell wall components Cell death Membrane integrity Redox status Tobacco cells |
| url | https://doi.org/10.1038/s41598-025-87762-6 |
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