Highly efficient construction of monkey blastoid capsules from aged somatic cells
Abstract Blastoids—blastocyst-like structures created in vitro—emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities,...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56447-z |
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| _version_ | 1832571533368754176 |
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| author | Junmo Wu Tianao Shao Zengli Tang Gaojing Liu Zhuoyao Li Yuxi Shi Yu Kang Jiawei Zuo Bo Zhao Guangyu Hu Jiaqi Liu Weizhi Ji Lei Zhang Yuyu Niu |
| author_facet | Junmo Wu Tianao Shao Zengli Tang Gaojing Liu Zhuoyao Li Yuxi Shi Yu Kang Jiawei Zuo Bo Zhao Guangyu Hu Jiaqi Liu Weizhi Ji Lei Zhang Yuyu Niu |
| author_sort | Junmo Wu |
| collection | DOAJ |
| description | Abstract Blastoids—blastocyst-like structures created in vitro—emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities, typically below 30%, has limited their utility. Prior research has predominantly utilized embryonic stem cells. In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. We also introduce a hydrogel-based microfluidics platform for the scalable and reproducible production of size-adjustable, biodegradable blastoid capsules, providing a stable 3D structure and mechanical protection. This advancement in the high-efficiency, scalable production of monkey blastoid capsules from reprogrammed aged somatic cells significantly enhances the study of embryonic development and holds promise for regenerative medicine. |
| format | Article |
| id | doaj-art-9a9927de7f40449a9b7d53035e61f930 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-9a9927de7f40449a9b7d53035e61f9302025-02-02T12:32:57ZengNature PortfolioNature Communications2041-17232025-01-0116111810.1038/s41467-025-56447-zHighly efficient construction of monkey blastoid capsules from aged somatic cellsJunmo Wu0Tianao Shao1Zengli Tang2Gaojing Liu3Zhuoyao Li4Yuxi Shi5Yu Kang6Jiawei Zuo7Bo Zhao8Guangyu Hu9Jiaqi Liu10Weizhi Ji11Lei Zhang12Yuyu Niu13State Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyKey Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyKey Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of SciencesState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyState Key Laboratory of Primate Biomedical Research; Institute of Primate Translational Medicine, Kunming University of Science and TechnologyAbstract Blastoids—blastocyst-like structures created in vitro—emerge as a valuable model for early embryonic development research. Non-human primates stem cell-derived blastoids are an ethically viable alternative to human counterparts, yet the low formation efficiency of monkey blastoid cavities, typically below 30%, has limited their utility. Prior research has predominantly utilized embryonic stem cells. In this work, we demonstrate the efficient generation of blastoids from induced pluripotent stem cells and somatic cell nuclear transfer embryonic stem cells derived from aged monkeys, achieving an 80% formation efficiency. We also introduce a hydrogel-based microfluidics platform for the scalable and reproducible production of size-adjustable, biodegradable blastoid capsules, providing a stable 3D structure and mechanical protection. This advancement in the high-efficiency, scalable production of monkey blastoid capsules from reprogrammed aged somatic cells significantly enhances the study of embryonic development and holds promise for regenerative medicine.https://doi.org/10.1038/s41467-025-56447-z |
| spellingShingle | Junmo Wu Tianao Shao Zengli Tang Gaojing Liu Zhuoyao Li Yuxi Shi Yu Kang Jiawei Zuo Bo Zhao Guangyu Hu Jiaqi Liu Weizhi Ji Lei Zhang Yuyu Niu Highly efficient construction of monkey blastoid capsules from aged somatic cells Nature Communications |
| title | Highly efficient construction of monkey blastoid capsules from aged somatic cells |
| title_full | Highly efficient construction of monkey blastoid capsules from aged somatic cells |
| title_fullStr | Highly efficient construction of monkey blastoid capsules from aged somatic cells |
| title_full_unstemmed | Highly efficient construction of monkey blastoid capsules from aged somatic cells |
| title_short | Highly efficient construction of monkey blastoid capsules from aged somatic cells |
| title_sort | highly efficient construction of monkey blastoid capsules from aged somatic cells |
| url | https://doi.org/10.1038/s41467-025-56447-z |
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