Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy
Lysosomes are pivotal in diverse physiological phenomena, encompassing autophagy, apoptosis, and cellular senescence. The demand for precise tumors treatment has led to the development of specific lysosome-targeting probes capable of elucidating lysosomal dynamics and facilitating targeted cell deat...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Photodiagnosis and Photodynamic Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024004678 |
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| author | Xiuqin Chen Guizhi Chen Sitong Cao Ruoxin Ye Ruoyi Qiu Xiangyu Yang Yiru Peng Hong Sun |
| author_facet | Xiuqin Chen Guizhi Chen Sitong Cao Ruoxin Ye Ruoyi Qiu Xiangyu Yang Yiru Peng Hong Sun |
| author_sort | Xiuqin Chen |
| collection | DOAJ |
| description | Lysosomes are pivotal in diverse physiological phenomena, encompassing autophagy, apoptosis, and cellular senescence. The demand for precise tumors treatment has led to the development of specific lysosome-targeting probes capable of elucidating lysosomal dynamics and facilitating targeted cell death. In this research, we report the synthesis and characterization of a novel benzopyrrolidinyl-substituted silicon phthalocyanine (Py-SiPc), designed for selective lysosome labeling and Fluorescence imaging-guided in vitro photodynamic therapy. Furthermore, we encapsulated Py-SiPc within a biocompatible nanocarrier, dipalmitoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE), to create water-soluble nanoparticles (DSPE@Py-SiPc). These nanoparticles exhibit exceptional lysosome labeling capabilities, as evidenced by bioimaging techniques. Upon exposure to laser irradiation, DSPE@Py-SiPc efficiently induces the production of reactive oxygen species, impairing lysosomal function and triggering lysosomal-mediated cell death. The DSPE@Py-SiPc system emerges as a promising photosensitizer. |
| format | Article |
| id | doaj-art-9a927a0daeb84ac6aa7d9f230024cff0 |
| institution | Kabale University |
| issn | 1572-1000 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-9a927a0daeb84ac6aa7d9f230024cff02025-02-01T04:11:41ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002025-02-0151104431Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapyXiuqin Chen0Guizhi Chen1Sitong Cao2Ruoxin Ye3Ruoyi Qiu4Xiangyu Yang5Yiru Peng6Hong Sun7College of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, China; Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, ChinaCollege of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, ChinaCollege of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, ChinaCollege of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, ChinaCollege of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, ChinaCollege of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, ChinaCollege of Chemistry & Materials, Fujian Provincial Key Laboratory of Advanced Materials Oriented Chemical Engineering, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University, Fuzhou, China; Corresponding authors.Department of Pharmacy, Fujian Provincial Hospital, Shengli Clinical Medical College of Fujian Medical University,Fuzhou University Affiliated Provincial Hospital, Fuzhou 350001, PR China; Corresponding authors.Lysosomes are pivotal in diverse physiological phenomena, encompassing autophagy, apoptosis, and cellular senescence. The demand for precise tumors treatment has led to the development of specific lysosome-targeting probes capable of elucidating lysosomal dynamics and facilitating targeted cell death. In this research, we report the synthesis and characterization of a novel benzopyrrolidinyl-substituted silicon phthalocyanine (Py-SiPc), designed for selective lysosome labeling and Fluorescence imaging-guided in vitro photodynamic therapy. Furthermore, we encapsulated Py-SiPc within a biocompatible nanocarrier, dipalmitoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE), to create water-soluble nanoparticles (DSPE@Py-SiPc). These nanoparticles exhibit exceptional lysosome labeling capabilities, as evidenced by bioimaging techniques. Upon exposure to laser irradiation, DSPE@Py-SiPc efficiently induces the production of reactive oxygen species, impairing lysosomal function and triggering lysosomal-mediated cell death. The DSPE@Py-SiPc system emerges as a promising photosensitizer.http://www.sciencedirect.com/science/article/pii/S1572100024004678Pyrrolidine ketonePhthalocyanineLysosomal targetingTwo-photon fluorescence imagingPhotodynamic therapy |
| spellingShingle | Xiuqin Chen Guizhi Chen Sitong Cao Ruoxin Ye Ruoyi Qiu Xiangyu Yang Yiru Peng Hong Sun Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy Photodiagnosis and Photodynamic Therapy Pyrrolidine ketone Phthalocyanine Lysosomal targeting Two-photon fluorescence imaging Photodynamic therapy |
| title | Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy |
| title_full | Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy |
| title_fullStr | Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy |
| title_full_unstemmed | Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy |
| title_short | Benzo-pyrrolidinyl substituted silicon phthalocyanines: A novel two-photon lysosomal nanoprobe for in vitro photodynamic therapy |
| title_sort | benzo pyrrolidinyl substituted silicon phthalocyanines a novel two photon lysosomal nanoprobe for in vitro photodynamic therapy |
| topic | Pyrrolidine ketone Phthalocyanine Lysosomal targeting Two-photon fluorescence imaging Photodynamic therapy |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024004678 |
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