Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet
We aimed to investigate whether swimming exercise could improve insulin resistance (IR) by regulating tripartite motif family protein 72 (TRIM72) expression and AKT signal pathway in rats fed with high-fat diet. Five-week-old rats were classified into 3 groups: standard diet as control (CON), high-f...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/1564386 |
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| _version_ | 1832548413982375936 |
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| author | Jie Qi Bo Yang Cailing Ren Jian Fu Jun Zhang |
| author_facet | Jie Qi Bo Yang Cailing Ren Jian Fu Jun Zhang |
| author_sort | Jie Qi |
| collection | DOAJ |
| description | We aimed to investigate whether swimming exercise could improve insulin resistance (IR) by regulating tripartite motif family protein 72 (TRIM72) expression and AKT signal pathway in rats fed with high-fat diet. Five-week-old rats were classified into 3 groups: standard diet as control (CON), high-fat diet (HFD), and HFD plus swimming exercise (Ex-HFD). After 8 weeks, glucose infusion rate (GIR), markers of oxidative stress, mRNA and protein expression of TRIM72, protein of IRS, p-AKTSer473, and AKT were determined in quadriceps muscles. Compared with HFD, the GIR, muscle SOD, and GSH-Px were significantly increased (p<0.05, resp.), whereas muscle MDA and 8-OHdG levels were significantly decreased (p<0.05 and p<0.01) in Ex-HFD. Expression levels of TRIM72 mRNA and protein in muscles were significantly reduced (p<0.05 and p<0.01), whereas protein expression levels of IRS-1, p-AKTSer473, and AKT were significantly increased in Ex-HFD compared with HFD (p<0.01, p<0.01, and p<0.05). These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. |
| format | Article |
| id | doaj-art-9a8f65035eb84d71b2e456d0c4aa2e22 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-9a8f65035eb84d71b2e456d0c4aa2e222025-02-03T06:14:06ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/15643861564386Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat DietJie Qi0Bo Yang1Cailing Ren2Jian Fu3Jun Zhang4College of Physical Education, Shanghai Normal University, Shanghai 200234, ChinaDepartment of Food Science and Nutrition, Zhejiang University, Hangzhou 310053, ChinaRehabilitation College of Gannan Medical University, Jiangxi 341000, ChinaCollege of Physical Education, Yangzhou University, Jiangsu 225009, ChinaCollege of Physical Education, Shanghai Normal University, Shanghai 200234, ChinaWe aimed to investigate whether swimming exercise could improve insulin resistance (IR) by regulating tripartite motif family protein 72 (TRIM72) expression and AKT signal pathway in rats fed with high-fat diet. Five-week-old rats were classified into 3 groups: standard diet as control (CON), high-fat diet (HFD), and HFD plus swimming exercise (Ex-HFD). After 8 weeks, glucose infusion rate (GIR), markers of oxidative stress, mRNA and protein expression of TRIM72, protein of IRS, p-AKTSer473, and AKT were determined in quadriceps muscles. Compared with HFD, the GIR, muscle SOD, and GSH-Px were significantly increased (p<0.05, resp.), whereas muscle MDA and 8-OHdG levels were significantly decreased (p<0.05 and p<0.01) in Ex-HFD. Expression levels of TRIM72 mRNA and protein in muscles were significantly reduced (p<0.05 and p<0.01), whereas protein expression levels of IRS-1, p-AKTSer473, and AKT were significantly increased in Ex-HFD compared with HFD (p<0.01, p<0.01, and p<0.05). These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction.http://dx.doi.org/10.1155/2016/1564386 |
| spellingShingle | Jie Qi Bo Yang Cailing Ren Jian Fu Jun Zhang Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet Journal of Diabetes Research |
| title | Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet |
| title_full | Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet |
| title_fullStr | Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet |
| title_full_unstemmed | Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet |
| title_short | Swimming Exercise Alleviated Insulin Resistance by Regulating Tripartite Motif Family Protein 72 Expression and AKT Signal Pathway in Sprague-Dawley Rats Fed with High-Fat Diet |
| title_sort | swimming exercise alleviated insulin resistance by regulating tripartite motif family protein 72 expression and akt signal pathway in sprague dawley rats fed with high fat diet |
| url | http://dx.doi.org/10.1155/2016/1564386 |
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