Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress
The phosphate transporter (PHT) family plays an important role in the uptake and transport of P elements in plants. A total of 158 <i>PbPHTs</i> were identified from the genome of ‘duli’ (<i>Pyrus betulifolia Bunge</i>) in this study, including 70 <i>PbPHT1s</i>,...
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2025-01-01
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| author | Shuai Yuan Weilong Zhang Yuxing Zhang |
| author_facet | Shuai Yuan Weilong Zhang Yuxing Zhang |
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| description | The phosphate transporter (PHT) family plays an important role in the uptake and transport of P elements in plants. A total of 158 <i>PbPHTs</i> were identified from the genome of ‘duli’ (<i>Pyrus betulifolia Bunge</i>) in this study, including 70 <i>PbPHT1s</i>, 2 <i>PbPHT2s</i>, 70 <i>PbPHT3s</i>, 12 <i>PbPHT4s</i>, and 4 <i>PbPHT5s</i>. Among the 158 <i>PHT</i> genes, 150 were localized to 17 ‘duli’ chromosomes. Gene duplication analysis identified 18 tandemly duplicated gene pairs. The promoter analysis showed that there were a large number of cis-acting elements related to phytohormones, growth, development, stress, and light response in <i>PbPHTs</i>. qRT-PCR analysis revealed that most <i>PHT</i> genes in ‘duli’ were highly expressed in the fruits, flowers, leaves, stems, and roots, and 15 <i>PbPHT</i> genes were responsive to 5 μM, 0.5 mM, 5 mM H<sub>2</sub>PO<sub>4</sub>, NaCl, GR24 (synthetic SL analog), GA3 (gibberellin 3), ABA (abscisic acid), and IAA (indole-3-acetic acid). GR24, GA3, IAA, and 5 mM KH<sub>2</sub>PO<sub>4</sub> treatments could increase the concentration, absorption, transport, and distribution of P elements in the rhizomes and leaves of ‘duli’, but 5 μM KH<sub>2</sub>PO<sub>4</sub>, NaCl, and ABA had the opposite effect. This study therefore provides a list of <i>PbPHT</i> genes with substantial roles in abiotic stress response, as well as important information to understand the functional characteristics of <i>PbPHT</i> during ‘duli’ abiotic stress tolerance, and explores the function of PbPHTs in exogenous hormones, phosphorus, and salt stress in the future. |
| format | Article |
| id | doaj-art-9a8bb2be27ff423ebacacd5740e73851 |
| institution | Kabale University |
| issn | 2077-0472 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Agriculture |
| spelling | doaj-art-9a8bb2be27ff423ebacacd5740e738512025-01-24T13:16:06ZengMDPI AGAgriculture2077-04722025-01-0115219910.3390/agriculture15020199Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt StressShuai Yuan0Weilong Zhang1Yuxing Zhang2College of Horticulture, Hebei Agricultural University, Baoding 071001, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, ChinaCollege of Horticulture, Hebei Agricultural University, Baoding 071001, ChinaThe phosphate transporter (PHT) family plays an important role in the uptake and transport of P elements in plants. A total of 158 <i>PbPHTs</i> were identified from the genome of ‘duli’ (<i>Pyrus betulifolia Bunge</i>) in this study, including 70 <i>PbPHT1s</i>, 2 <i>PbPHT2s</i>, 70 <i>PbPHT3s</i>, 12 <i>PbPHT4s</i>, and 4 <i>PbPHT5s</i>. Among the 158 <i>PHT</i> genes, 150 were localized to 17 ‘duli’ chromosomes. Gene duplication analysis identified 18 tandemly duplicated gene pairs. The promoter analysis showed that there were a large number of cis-acting elements related to phytohormones, growth, development, stress, and light response in <i>PbPHTs</i>. qRT-PCR analysis revealed that most <i>PHT</i> genes in ‘duli’ were highly expressed in the fruits, flowers, leaves, stems, and roots, and 15 <i>PbPHT</i> genes were responsive to 5 μM, 0.5 mM, 5 mM H<sub>2</sub>PO<sub>4</sub>, NaCl, GR24 (synthetic SL analog), GA3 (gibberellin 3), ABA (abscisic acid), and IAA (indole-3-acetic acid). GR24, GA3, IAA, and 5 mM KH<sub>2</sub>PO<sub>4</sub> treatments could increase the concentration, absorption, transport, and distribution of P elements in the rhizomes and leaves of ‘duli’, but 5 μM KH<sub>2</sub>PO<sub>4</sub>, NaCl, and ABA had the opposite effect. This study therefore provides a list of <i>PbPHT</i> genes with substantial roles in abiotic stress response, as well as important information to understand the functional characteristics of <i>PbPHT</i> during ‘duli’ abiotic stress tolerance, and explores the function of PbPHTs in exogenous hormones, phosphorus, and salt stress in the future.https://www.mdpi.com/2077-0472/15/2/199‘duli’PHTgene expression analysishormonalabiotic stressphysiological change |
| spellingShingle | Shuai Yuan Weilong Zhang Yuxing Zhang Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress Agriculture ‘duli’ PHT gene expression analysis hormonal abiotic stress physiological change |
| title | Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress |
| title_full | Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress |
| title_fullStr | Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress |
| title_full_unstemmed | Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress |
| title_short | Characterization of <i>PHT</i> Genes in ‘duli’ (<i>Pyrus betulifolia Bunge</i>) and Expression Analysis of <i>PbPHTs</i> in Response to Plant Growth Regulators, P, and Salt Stress |
| title_sort | characterization of i pht i genes in duli i pyrus betulifolia bunge i and expression analysis of i pbphts i in response to plant growth regulators p and salt stress |
| topic | ‘duli’ PHT gene expression analysis hormonal abiotic stress physiological change |
| url | https://www.mdpi.com/2077-0472/15/2/199 |
| work_keys_str_mv | AT shuaiyuan characterizationofiphtigenesinduliipyrusbetulifoliabungeiandexpressionanalysisofipbphtsiinresponsetoplantgrowthregulatorspandsaltstress AT weilongzhang characterizationofiphtigenesinduliipyrusbetulifoliabungeiandexpressionanalysisofipbphtsiinresponsetoplantgrowthregulatorspandsaltstress AT yuxingzhang characterizationofiphtigenesinduliipyrusbetulifoliabungeiandexpressionanalysisofipbphtsiinresponsetoplantgrowthregulatorspandsaltstress |