Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors
Background Glucose deprivation inhibits T-cell metabolism and function. Glucose levels are low in the tumor microenvironment of solid tumors and insufficient glucose uptake limits the antitumor response of T cells. Furthermore, glucose restriction can contribute to the failure of chimeric antigen re...
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| Format: | Article |
| Language: | English |
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BMJ Publishing Group
2025-01-01
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| Series: | Journal for ImmunoTherapy of Cancer |
| Online Access: | https://jitc.bmj.com/content/13/1/e010540.full |
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| author | Hui Wang Yi Zhang Feng Li Shasha Liu Shumin Wang Wenhao Hu Yue Liang Chunyi Shen Yuyu Zhao |
| author_facet | Hui Wang Yi Zhang Feng Li Shasha Liu Shumin Wang Wenhao Hu Yue Liang Chunyi Shen Yuyu Zhao |
| author_sort | Hui Wang |
| collection | DOAJ |
| description | Background Glucose deprivation inhibits T-cell metabolism and function. Glucose levels are low in the tumor microenvironment of solid tumors and insufficient glucose uptake limits the antitumor response of T cells. Furthermore, glucose restriction can contribute to the failure of chimeric antigen receptor T (CAR-T) cell therapy for solid tumors. However, the impact of glucose restriction remains unknown in CAR-T cell therapy.Methods Glucose transporters were detected and overexpressed in CAR-T cells. The impacts of glucose restriction on CAR-T cells were checked in vitro and in vivo.Results Glucose restriction significantly decreased CAR-T cell activation, effector function, and expansion. CAR-T cells expressed high levels of the glucose transporter Glut1, which has a low affinity for glucose. Overexpression of Glut1 failed to improve CAR-T cell function under glucose-restricted conditions. In contrast, the function and antitumor potential of CAR-T cells was enhanced by the overexpression of Glut3, which has the highest affinity for glucose among the Glut transporter family and is expressed in minor parts of CAR-T cells. Glut3-overexpressing CAR-T cells demonstrated increased tumoricidal efficacy in multiple xenografts and syngenetic mouse models. Furthermore, Glut3 overexpression activated the PI3K/Akt pathway and increased OXPHOS and mitochondrial fitness.Conclusions We provide a direct and effective approach to enhance low glucose uptake levels by CAR-T cells and improve their antitumor efficacy against solid tumors. |
| format | Article |
| id | doaj-art-9a54571a03c7451fbc02271c652516b4 |
| institution | Kabale University |
| issn | 2051-1426 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMJ Publishing Group |
| record_format | Article |
| series | Journal for ImmunoTherapy of Cancer |
| spelling | doaj-art-9a54571a03c7451fbc02271c652516b42025-01-20T00:55:12ZengBMJ Publishing GroupJournal for ImmunoTherapy of Cancer2051-14262025-01-0113110.1136/jitc-2024-010540Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumorsHui Wang0Yi Zhang1Feng Li2Shasha Liu3Shumin Wang4Wenhao Hu5Yue Liang6Chunyi Shen7Yuyu Zhao8Biotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBiotherapy Center & Cancer Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, People`s Republic of ChinaBackground Glucose deprivation inhibits T-cell metabolism and function. Glucose levels are low in the tumor microenvironment of solid tumors and insufficient glucose uptake limits the antitumor response of T cells. Furthermore, glucose restriction can contribute to the failure of chimeric antigen receptor T (CAR-T) cell therapy for solid tumors. However, the impact of glucose restriction remains unknown in CAR-T cell therapy.Methods Glucose transporters were detected and overexpressed in CAR-T cells. The impacts of glucose restriction on CAR-T cells were checked in vitro and in vivo.Results Glucose restriction significantly decreased CAR-T cell activation, effector function, and expansion. CAR-T cells expressed high levels of the glucose transporter Glut1, which has a low affinity for glucose. Overexpression of Glut1 failed to improve CAR-T cell function under glucose-restricted conditions. In contrast, the function and antitumor potential of CAR-T cells was enhanced by the overexpression of Glut3, which has the highest affinity for glucose among the Glut transporter family and is expressed in minor parts of CAR-T cells. Glut3-overexpressing CAR-T cells demonstrated increased tumoricidal efficacy in multiple xenografts and syngenetic mouse models. Furthermore, Glut3 overexpression activated the PI3K/Akt pathway and increased OXPHOS and mitochondrial fitness.Conclusions We provide a direct and effective approach to enhance low glucose uptake levels by CAR-T cells and improve their antitumor efficacy against solid tumors.https://jitc.bmj.com/content/13/1/e010540.full |
| spellingShingle | Hui Wang Yi Zhang Feng Li Shasha Liu Shumin Wang Wenhao Hu Yue Liang Chunyi Shen Yuyu Zhao Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors Journal for ImmunoTherapy of Cancer |
| title | Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors |
| title_full | Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors |
| title_fullStr | Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors |
| title_full_unstemmed | Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors |
| title_short | Glut3 overexpression improves environmental glucose uptake and antitumor efficacy of CAR-T cells in solid tumors |
| title_sort | glut3 overexpression improves environmental glucose uptake and antitumor efficacy of car t cells in solid tumors |
| url | https://jitc.bmj.com/content/13/1/e010540.full |
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