Protocol to analyze tRNA and rRNA processing using biotin-labeled probes

Protocol to analyze tRNA and rRNA processing using biotin-labeled probes

Summary: Mature tRNAs and rRNAs are derived from larger precursor transcripts through intricate endo- and exonuclease processing steps. Here, we present a protocol to analyze rRNA and tRNA processing using total RNA extracted from human cells. We describe steps for separating RNA by denaturing elect...

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Bibliographic Details
Main Authors: Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Genetics
Molecular Biology
Molecular/Chemical Probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725002722
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