Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi
The production of interferon-γ and tumour necrosis factor-α was evaluated in the peripheral blood mononuclear cells (PBMCs) from healthy donors and AIDS patients after Rhodococcus equi infection in vitro. PBMCs from healthy donors secreted elevated levels of IFN-γ and TNF-α when challenged in vitro...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1995-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935195000494 |
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| _version_ | 1832545525840216064 |
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| author | S. Delia C. M. Mastroianni M. Lichtner F. Mengoni S. Moretti V. Vullo |
| author_facet | S. Delia C. M. Mastroianni M. Lichtner F. Mengoni S. Moretti V. Vullo |
| author_sort | S. Delia |
| collection | DOAJ |
| description | The production of interferon-γ and tumour necrosis factor-α was evaluated in the peripheral blood mononuclear cells (PBMCs) from healthy donors and AIDS patients after Rhodococcus equi infection in vitro. PBMCs from healthy donors secreted elevated levels of IFN-γ and TNF-α when challenged in vitro with killed R. equi, whereas the release of both cytokines was impaired in supernatant cultures from AIDS patients. We conclude that the failure of IFN-γ generation in AIDS patients in response to R. equi is not antigen-specific but it may reflect the global impairment of T-cell function. In such patients, however, the infection with R. equi, a facultative intracellular pathogen which survives and replicates within macrophages, may be responsible for the impairment in the TNF-α release, possibly enhancing the HIV-induced macrophage dysftmction. |
| format | Article |
| id | doaj-art-9a4e481ac05e481a97a37a3f696d8b12 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1995-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9a4e481ac05e481a97a37a3f696d8b122025-02-03T07:25:38ZengWileyMediators of Inflammation0962-93511466-18611995-01-014430630910.1155/S0962935195000494Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equiS. Delia0C. M. Mastroianni1M. Lichtner2F. Mengoni3S. Moretti4V. Vullo5Department of Infectious and Tropical Diseases, La Sapienza University, Rome, ItalyDepartment of Infectious and Tropical Diseases, La Sapienza University, Rome, ItalyDepartment of Infectious and Tropical Diseases, La Sapienza University, Rome, ItalyDepartment of Infectious and Tropical Diseases, La Sapienza University, Rome, ItalyDepartment of Infectious and Tropical Diseases, La Sapienza University, Rome, ItalyDepartment of Infectious and Tropical Diseases, La Sapienza University, Rome, ItalyThe production of interferon-γ and tumour necrosis factor-α was evaluated in the peripheral blood mononuclear cells (PBMCs) from healthy donors and AIDS patients after Rhodococcus equi infection in vitro. PBMCs from healthy donors secreted elevated levels of IFN-γ and TNF-α when challenged in vitro with killed R. equi, whereas the release of both cytokines was impaired in supernatant cultures from AIDS patients. We conclude that the failure of IFN-γ generation in AIDS patients in response to R. equi is not antigen-specific but it may reflect the global impairment of T-cell function. In such patients, however, the infection with R. equi, a facultative intracellular pathogen which survives and replicates within macrophages, may be responsible for the impairment in the TNF-α release, possibly enhancing the HIV-induced macrophage dysftmction.http://dx.doi.org/10.1155/S0962935195000494 |
| spellingShingle | S. Delia C. M. Mastroianni M. Lichtner F. Mengoni S. Moretti V. Vullo Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi Mediators of Inflammation |
| title | Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi |
| title_full | Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi |
| title_fullStr | Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi |
| title_full_unstemmed | Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi |
| title_short | Defective production of interferon-γ and tumour necrosis factor-α by AIDS mononuclear cells after in vitro exposure to Rhodococcus equi |
| title_sort | defective production of interferon γ and tumour necrosis factor α by aids mononuclear cells after in vitro exposure to rhodococcus equi |
| url | http://dx.doi.org/10.1155/S0962935195000494 |
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