In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B

Objectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization of the interfacial gap may ultimately lead to infection and bone resorption. Using Rhodamine B, a sensiti...

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Main Authors: Antoine Berberi, Georges Tehini, Khaldoun Rifai, Farah Bou Nasser Eddine, Nabil El Zein, Bassam Badran, Haidar Akl
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2014/351263
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author Antoine Berberi
Georges Tehini
Khaldoun Rifai
Farah Bou Nasser Eddine
Nabil El Zein
Bassam Badran
Haidar Akl
author_facet Antoine Berberi
Georges Tehini
Khaldoun Rifai
Farah Bou Nasser Eddine
Nabil El Zein
Bassam Badran
Haidar Akl
author_sort Antoine Berberi
collection DOAJ
description Objectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization of the interfacial gap may ultimately lead to infection and bone resorption. Using Rhodamine B, a sensitive fluorescent tracer dye, we aim in this study to investigate leakage at implant-abutment connection of three implant systems having the same prosthetic interface. Materials and Methods. Twenty-one implants (seven Astra Tech, seven Euroteknika, and seven Dentium) with the same prosthetic interface were connected to their original abutments, according to the manufacturers’ recommendation. After determination of the inner volume of each implant systems, the kinetic quantification of leakage was evaluated for each group using Rhodamine B (10−2 M). For each group, spectrophotometric analysis was performed to detect leakage with a fluorescence spectrophotometer at 1 h (T0) and 48 h (T1) of incubation time at room temperature. Results. Astra Tech had the highest inner volume (6.8 μL), compared to Dentium (4 μL) and Euroteknika (2.9 μL). At T0 and T1, respectively, the leakage volume and percentage of each system were as follows: Astra Tech 0.043 μL or 1.48% (SD 0.0022), 0.08 μL or 5.56% (SD 0.0074), Euroteknika 0.09 μL or 6.93% (SD 0.0913), 0.21 μL or 20.55% (SD 0.0035), and Dentium 0.07 μL or 4.6% (SD 0.0029), 0.12 μL or 10.47% (SD 0.0072). Conclusion. The tested internal conical implant-abutment connections appear to be unable to prevent leakage. In average, Astra Tech implants showed the highest inner volume and the least leakage.
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spelling doaj-art-9a3f7e4e90444ac89cd61d7e1d3f27f22025-02-03T06:11:33ZengWileyInternational Journal of Dentistry1687-87281687-87362014-01-01201410.1155/2014/351263351263In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine BAntoine Berberi0Georges Tehini1Khaldoun Rifai2Farah Bou Nasser Eddine3Nabil El Zein4Bassam Badran5Haidar Akl6School of Dentistry, Lebanese University, P.O. Box 4, Hadath, LebanonSchool of Dentistry, Lebanese University, P.O. Box 4, Hadath, LebanonSchool of Dentistry, Lebanese University, P.O. Box 4, Hadath, LebanonEcole Doctorale, PRASE, Lebanese University, P.O. Box 4, Hadath, LebanonEcole Doctorale, PRASE, Lebanese University, P.O. Box 4, Hadath, LebanonEcole Doctorale, PRASE, Lebanese University, P.O. Box 4, Hadath, LebanonEcole Doctorale, PRASE, Lebanese University, P.O. Box 4, Hadath, LebanonObjectives. Hollow space between implant and abutment may act as reservoir for commensal and/or pathogenic bacteria representing a potential source of tissue inflammation. Microbial colonization of the interfacial gap may ultimately lead to infection and bone resorption. Using Rhodamine B, a sensitive fluorescent tracer dye, we aim in this study to investigate leakage at implant-abutment connection of three implant systems having the same prosthetic interface. Materials and Methods. Twenty-one implants (seven Astra Tech, seven Euroteknika, and seven Dentium) with the same prosthetic interface were connected to their original abutments, according to the manufacturers’ recommendation. After determination of the inner volume of each implant systems, the kinetic quantification of leakage was evaluated for each group using Rhodamine B (10−2 M). For each group, spectrophotometric analysis was performed to detect leakage with a fluorescence spectrophotometer at 1 h (T0) and 48 h (T1) of incubation time at room temperature. Results. Astra Tech had the highest inner volume (6.8 μL), compared to Dentium (4 μL) and Euroteknika (2.9 μL). At T0 and T1, respectively, the leakage volume and percentage of each system were as follows: Astra Tech 0.043 μL or 1.48% (SD 0.0022), 0.08 μL or 5.56% (SD 0.0074), Euroteknika 0.09 μL or 6.93% (SD 0.0913), 0.21 μL or 20.55% (SD 0.0035), and Dentium 0.07 μL or 4.6% (SD 0.0029), 0.12 μL or 10.47% (SD 0.0072). Conclusion. The tested internal conical implant-abutment connections appear to be unable to prevent leakage. In average, Astra Tech implants showed the highest inner volume and the least leakage.http://dx.doi.org/10.1155/2014/351263
spellingShingle Antoine Berberi
Georges Tehini
Khaldoun Rifai
Farah Bou Nasser Eddine
Nabil El Zein
Bassam Badran
Haidar Akl
In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B
International Journal of Dentistry
title In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B
title_full In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B
title_fullStr In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B
title_full_unstemmed In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B
title_short In Vitro Evaluation of Leakage at Implant-Abutment Connection of Three Implant Systems Having the Same Prosthetic Interface Using Rhodamine B
title_sort in vitro evaluation of leakage at implant abutment connection of three implant systems having the same prosthetic interface using rhodamine b
url http://dx.doi.org/10.1155/2014/351263
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