Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil
Agricultural improvement of seminatural grasslands has been shown to result in changes to plant and microbial diversity, with consequences for ecosystem functioning. A microcosm approach was used to elucidate the effects of two key components of agricultural improvement (nitrogen addition and liming...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Applied and Environmental Soil Science |
| Online Access: | http://dx.doi.org/10.1155/2010/319721 |
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| author | Deirdre C. Rooney Nabla M. Kennedy Deirdre B. Gleeson Nicholas J. W. Clipson |
| author_facet | Deirdre C. Rooney Nabla M. Kennedy Deirdre B. Gleeson Nicholas J. W. Clipson |
| author_sort | Deirdre C. Rooney |
| collection | DOAJ |
| description | Agricultural improvement of seminatural grasslands has been shown to result in changes to plant and microbial diversity, with consequences for ecosystem functioning. A microcosm approach was used to elucidate the effects of two key components of agricultural improvement (nitrogen addition and liming) on ammonia-oxidising bacterial (AOB) communities in an upland grassland soil. Plant species characteristic of unimproved and improved pastures (A. capillaris and L. perenne) were planted in microcosms, and lime, nitrogen (NH4NO3), or lime plus nitrogen added. The AOB community was profiled using terminal restriction fragment length polymorphism (TRFLP) of the amoA gene. AOB community structure was largely altered by NH4NO3 addition, rather than liming, although interactions between nitrogen addition and plant species were also evident. Results indicate that nitrogen addition drives shifts in the structure of key microbial communities in upland grassland soils, and that plant species may play a significant role in determining AOB community structure. |
| format | Article |
| id | doaj-art-9a0f894d5c4e4949bd697c7cc747787f |
| institution | Kabale University |
| issn | 1687-7667 1687-7675 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applied and Environmental Soil Science |
| spelling | doaj-art-9a0f894d5c4e4949bd697c7cc747787f2025-02-03T01:01:14ZengWileyApplied and Environmental Soil Science1687-76671687-76752010-01-01201010.1155/2010/319721319721Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland SoilDeirdre C. Rooney0Nabla M. Kennedy1Deirdre B. Gleeson2Nicholas J. W. Clipson3Microbial Ecology Group, School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, IrelandMicrobial Ecology Group, School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, IrelandSoil Biology Group, School of Earth and Environment (M087), The University of Western Australia, 35 Stirling Highway, WA 6009 Crawley, AustraliaMicrobial Ecology Group, School of Biology and Environmental Science, University College Dublin, Belfield, Dublin 4, IrelandAgricultural improvement of seminatural grasslands has been shown to result in changes to plant and microbial diversity, with consequences for ecosystem functioning. A microcosm approach was used to elucidate the effects of two key components of agricultural improvement (nitrogen addition and liming) on ammonia-oxidising bacterial (AOB) communities in an upland grassland soil. Plant species characteristic of unimproved and improved pastures (A. capillaris and L. perenne) were planted in microcosms, and lime, nitrogen (NH4NO3), or lime plus nitrogen added. The AOB community was profiled using terminal restriction fragment length polymorphism (TRFLP) of the amoA gene. AOB community structure was largely altered by NH4NO3 addition, rather than liming, although interactions between nitrogen addition and plant species were also evident. Results indicate that nitrogen addition drives shifts in the structure of key microbial communities in upland grassland soils, and that plant species may play a significant role in determining AOB community structure.http://dx.doi.org/10.1155/2010/319721 |
| spellingShingle | Deirdre C. Rooney Nabla M. Kennedy Deirdre B. Gleeson Nicholas J. W. Clipson Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil Applied and Environmental Soil Science |
| title | Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil |
| title_full | Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil |
| title_fullStr | Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil |
| title_full_unstemmed | Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil |
| title_short | Responses of Ammonia-Oxidising Bacterial Communities to Nitrogen, Lime, and Plant Species in Upland Grassland Soil |
| title_sort | responses of ammonia oxidising bacterial communities to nitrogen lime and plant species in upland grassland soil |
| url | http://dx.doi.org/10.1155/2010/319721 |
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