Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry

The cider-making industry in Asturias generates between 9000 and 12,000 tons of apple pomace per year. This by-product, the remains of the apple pressing, and made up of peel, flesh, seeds and stems, is a valuable material, containing substantial amounts of antioxidant compounds associated with heal...

Full description

Saved in:
Bibliographic Details
Main Authors: Rosa Pando Bedriñana, Roberto Rodríguez Madrera, María Dolores Loureiro Rodríguez, Karelmar López-Benítez, Anna Picinelli Lobo
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Antioxidants
Subjects:
Online Access:https://www.mdpi.com/2076-3921/13/10/1230
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850205073470128128
author Rosa Pando Bedriñana
Roberto Rodríguez Madrera
María Dolores Loureiro Rodríguez
Karelmar López-Benítez
Anna Picinelli Lobo
author_facet Rosa Pando Bedriñana
Roberto Rodríguez Madrera
María Dolores Loureiro Rodríguez
Karelmar López-Benítez
Anna Picinelli Lobo
author_sort Rosa Pando Bedriñana
collection DOAJ
description The cider-making industry in Asturias generates between 9000 and 12,000 tons of apple pomace per year. This by-product, the remains of the apple pressing, and made up of peel, flesh, seeds and stems, is a valuable material, containing substantial amounts of antioxidant compounds associated with healthy properties. Polyphenols such as dihydrochalcones and quercetin glycosides, and triterpenic acids, among which ursolic acid is a major compound, are the main antioxidant families described in apple pomace. The simultaneous recovery of those families has been accomplished by low frequency ultrasound-assisted extraction. Working extraction conditions were optimised by response surface methodology (RSM): time, 5.1 min; extractant composition, 68% ethanol in water; solid/liquid ratio, 1/75 and ultrasonic wave amplitude, 90%. This procedure was further applied to analyse those components in the whole apple pomace (WAP), apple peel (AP) and apple flesh (AF). On average, dry WAP contained almost 1300 µg/g of flavonols, 1200 µg/g of dihydrochalcones and 4200 µg/g of ursolic acid. These figures increased in the apple peel to, respectively 2500, 1400 and 8500 µg/g dry matter. Two linear multivariate regression models allowed the antioxidant activity of apple by-products to be predicted on the basis of their bioactive composition. The results derived from this study confirm the potential of industrial cider apple pomace as a source of high-value bioactive compounds, and the feasibility of the ultrasound-assisted extraction technique to recover those components in a simple and efficient way.
format Article
id doaj-art-9a02b7cdfa3e4e4a8a5e260b569670dc
institution OA Journals
issn 2076-3921
language English
publishDate 2024-10-01
publisher MDPI AG
record_format Article
series Antioxidants
spelling doaj-art-9a02b7cdfa3e4e4a8a5e260b569670dc2025-08-20T02:11:11ZengMDPI AGAntioxidants2076-39212024-10-011310123010.3390/antiox13101230Green Extraction of Bioactive Compounds from Apple Pomace from the Cider IndustryRosa Pando Bedriñana0Roberto Rodríguez Madrera1María Dolores Loureiro Rodríguez2Karelmar López-Benítez3Anna Picinelli Lobo4Area of Food Technology, Regional Agrifood Research and Development Center (SERIDA), Carretera AS267, PK19, Villaviciosa, 33300 Asturias, SpainArea of Food Technology, Regional Agrifood Research and Development Center (SERIDA), Carretera AS267, PK19, Villaviciosa, 33300 Asturias, SpainEstación de Viticultura e Enoloxía de Galicia, Ponte San Clodio s/n, Leiro, 32428 Orense, SpainArea of Food Technology, Regional Agrifood Research and Development Center (SERIDA), Carretera AS267, PK19, Villaviciosa, 33300 Asturias, SpainArea of Food Technology, Regional Agrifood Research and Development Center (SERIDA), Carretera AS267, PK19, Villaviciosa, 33300 Asturias, SpainThe cider-making industry in Asturias generates between 9000 and 12,000 tons of apple pomace per year. This by-product, the remains of the apple pressing, and made up of peel, flesh, seeds and stems, is a valuable material, containing substantial amounts of antioxidant compounds associated with healthy properties. Polyphenols such as dihydrochalcones and quercetin glycosides, and triterpenic acids, among which ursolic acid is a major compound, are the main antioxidant families described in apple pomace. The simultaneous recovery of those families has been accomplished by low frequency ultrasound-assisted extraction. Working extraction conditions were optimised by response surface methodology (RSM): time, 5.1 min; extractant composition, 68% ethanol in water; solid/liquid ratio, 1/75 and ultrasonic wave amplitude, 90%. This procedure was further applied to analyse those components in the whole apple pomace (WAP), apple peel (AP) and apple flesh (AF). On average, dry WAP contained almost 1300 µg/g of flavonols, 1200 µg/g of dihydrochalcones and 4200 µg/g of ursolic acid. These figures increased in the apple peel to, respectively 2500, 1400 and 8500 µg/g dry matter. Two linear multivariate regression models allowed the antioxidant activity of apple by-products to be predicted on the basis of their bioactive composition. The results derived from this study confirm the potential of industrial cider apple pomace as a source of high-value bioactive compounds, and the feasibility of the ultrasound-assisted extraction technique to recover those components in a simple and efficient way.https://www.mdpi.com/2076-3921/13/10/1230polyphenolstriterpenic acidsultrasound-assisted extractionapple by-products
spellingShingle Rosa Pando Bedriñana
Roberto Rodríguez Madrera
María Dolores Loureiro Rodríguez
Karelmar López-Benítez
Anna Picinelli Lobo
Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
Antioxidants
polyphenols
triterpenic acids
ultrasound-assisted extraction
apple by-products
title Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
title_full Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
title_fullStr Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
title_full_unstemmed Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
title_short Green Extraction of Bioactive Compounds from Apple Pomace from the Cider Industry
title_sort green extraction of bioactive compounds from apple pomace from the cider industry
topic polyphenols
triterpenic acids
ultrasound-assisted extraction
apple by-products
url https://www.mdpi.com/2076-3921/13/10/1230
work_keys_str_mv AT rosapandobedrinana greenextractionofbioactivecompoundsfromapplepomacefromtheciderindustry
AT robertorodriguezmadrera greenextractionofbioactivecompoundsfromapplepomacefromtheciderindustry
AT mariadoloresloureirorodriguez greenextractionofbioactivecompoundsfromapplepomacefromtheciderindustry
AT karelmarlopezbenitez greenextractionofbioactivecompoundsfromapplepomacefromtheciderindustry
AT annapicinellilobo greenextractionofbioactivecompoundsfromapplepomacefromtheciderindustry