Culture and Characterization of Microglia from the Adult Murine Retina
Purpose. To develop a protocol for isolating and culturing murine adult retinal microglia and to characterize the phenotype and function of the cultured cells. Method. Retinal single-cell suspensions were prepared from adult MF1 mice. Culture conditions including culture medium, growth factors, seed...
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Main Authors: | Gayathri Devarajan, Mei Chen, Elizabeth Muckersie, Heping Xu |
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Format: | Article |
Language: | English |
Published: |
Wiley
2014-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1155/2014/894368 |
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