Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates
Abstract Fluorescence signal “turn‐on” lateral flow immunoassay (FONLFA) through nanomaterial labeled quenching fluorescent nanomaterial has shown significant potential for the detection of small molecules. However, the fluorescent nanomaterial immobilization on nitrocellulose (NC) membrane commonly...
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2025-01-01
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| Online Access: | https://doi.org/10.1002/agt2.644 |
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| author | Mengjia Chao Shengmei Tai Minxin Mao Wenbo Cao Chifang Peng Wei Ma Yongwei Feng Zhouping Wang |
| author_facet | Mengjia Chao Shengmei Tai Minxin Mao Wenbo Cao Chifang Peng Wei Ma Yongwei Feng Zhouping Wang |
| author_sort | Mengjia Chao |
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| description | Abstract Fluorescence signal “turn‐on” lateral flow immunoassay (FONLFA) through nanomaterial labeled quenching fluorescent nanomaterial has shown significant potential for the detection of small molecules. However, the fluorescent nanomaterial immobilization on nitrocellulose (NC) membrane commonly requires tedious chemical modification and only a few combinations of fluorescence donor and quencher have been applied in FONLFA. In this work, bright fluorescent metal nanoclusters (Prot‐AuNCs) were prepared and self‐assembled into Prot‐AuNCs/antigen aggregates with three typical small molecule antigens, respectively. The aggregates can be readily immobilized on the surface of the NC membrane, indicating that this strip fabrication strategy has good versatility. Moreover, we evaluated the performances of this FONLFA platform by using carbendazim as a model target and investigated four typical nanomaterials as colorimetric nanoprobes and fluorescence quenchers. We found that all the nanoprobes demonstrated significantly improved naked eye detection sensitivity (vLOD) and limits of detection (LODs) in quantitative analysis. Among them, combing the Fe‐polydopamine nanoparticles as quencher with the above aggregates, the FONLFA in signal “turn‐on” mode achieved 200‐fold improved vLOD (0.05 ng mL−1) compared with conventional colorimetric AuNPs‐based lateral flow immunoassay (AuNPs‐LFA) (10 ng mL−1). In addition, the LOD in quantitative analysis also was improved by 22‐fold and the whole test process was completed within 10 min. With the advantages of efficient fabrication, extraordinary sensitization, and good biocompatibility, our FONLFA platform is expected to have great potential in the rapid detection of various small molecules. |
| format | Article |
| id | doaj-art-99e683a1581a4691ad8f5d6511e94ed3 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
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| spelling | doaj-art-99e683a1581a4691ad8f5d6511e94ed32025-01-21T08:57:07ZengWileyAggregate2692-45602025-01-0161n/an/a10.1002/agt2.644Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregatesMengjia Chao0Shengmei Tai1Minxin Mao2Wenbo Cao3Chifang Peng4Wei Ma5Yongwei Feng6Zhouping Wang7State Key Laboratory of Food Science and ResourcesJiangnan UniversityWuxiP. R. ChinaState Key Laboratory of Food Science and ResourcesJiangnan UniversityWuxiP. R. ChinaShandong Institute of PomologyTaianP. R. ChinaTechnology Innovation Center of Special Food for State Market RegulationWuxiP. R. ChinaState Key Laboratory of Food Science and ResourcesJiangnan UniversityWuxiP. R. ChinaState Key Laboratory of Food Science and ResourcesJiangnan UniversityWuxiP. R. ChinaTechnology Innovation Center of Special Food for State Market RegulationWuxiP. R. ChinaState Key Laboratory of Food Science and ResourcesJiangnan UniversityWuxiP. R. ChinaAbstract Fluorescence signal “turn‐on” lateral flow immunoassay (FONLFA) through nanomaterial labeled quenching fluorescent nanomaterial has shown significant potential for the detection of small molecules. However, the fluorescent nanomaterial immobilization on nitrocellulose (NC) membrane commonly requires tedious chemical modification and only a few combinations of fluorescence donor and quencher have been applied in FONLFA. In this work, bright fluorescent metal nanoclusters (Prot‐AuNCs) were prepared and self‐assembled into Prot‐AuNCs/antigen aggregates with three typical small molecule antigens, respectively. The aggregates can be readily immobilized on the surface of the NC membrane, indicating that this strip fabrication strategy has good versatility. Moreover, we evaluated the performances of this FONLFA platform by using carbendazim as a model target and investigated four typical nanomaterials as colorimetric nanoprobes and fluorescence quenchers. We found that all the nanoprobes demonstrated significantly improved naked eye detection sensitivity (vLOD) and limits of detection (LODs) in quantitative analysis. Among them, combing the Fe‐polydopamine nanoparticles as quencher with the above aggregates, the FONLFA in signal “turn‐on” mode achieved 200‐fold improved vLOD (0.05 ng mL−1) compared with conventional colorimetric AuNPs‐based lateral flow immunoassay (AuNPs‐LFA) (10 ng mL−1). In addition, the LOD in quantitative analysis also was improved by 22‐fold and the whole test process was completed within 10 min. With the advantages of efficient fabrication, extraordinary sensitization, and good biocompatibility, our FONLFA platform is expected to have great potential in the rapid detection of various small molecules.https://doi.org/10.1002/agt2.644Fe‐polydopamine nanoparticlesgold nanoclusterslateral flow assayself‐assemblysignal “turn‐on”small molecules |
| spellingShingle | Mengjia Chao Shengmei Tai Minxin Mao Wenbo Cao Chifang Peng Wei Ma Yongwei Feng Zhouping Wang Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates Aggregate Fe‐polydopamine nanoparticles gold nanoclusters lateral flow assay self‐assembly signal “turn‐on” small molecules |
| title | Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates |
| title_full | Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates |
| title_fullStr | Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates |
| title_full_unstemmed | Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates |
| title_short | Versatile and efficient fabrication of signal “turn‐on” lateral flow assay for ultrasensitive naked eye detection of small molecules based on self‐assembled fluorescent gold nanoclusters‐antigen aggregates |
| title_sort | versatile and efficient fabrication of signal turn on lateral flow assay for ultrasensitive naked eye detection of small molecules based on self assembled fluorescent gold nanoclusters antigen aggregates |
| topic | Fe‐polydopamine nanoparticles gold nanoclusters lateral flow assay self‐assembly signal “turn‐on” small molecules |
| url | https://doi.org/10.1002/agt2.644 |
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