Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification
Abstract DNA barcode detection method is widely applied for species identification, which is imperative to evaluate the effect of human economic activities on the biodiversity of ecosystem. However, the wide utilization of existing detection biosensors is limited by bulky and expensive instruments,...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202408718 |
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| author | Wenlong Ma Jiawei Li Xiaolin Qu Shao‘e Sun Yanan Zhou Yitong Liu Peng Wang Zhongli Sha |
| author_facet | Wenlong Ma Jiawei Li Xiaolin Qu Shao‘e Sun Yanan Zhou Yitong Liu Peng Wang Zhongli Sha |
| author_sort | Wenlong Ma |
| collection | DOAJ |
| description | Abstract DNA barcode detection method is widely applied for species identification, which is imperative to evaluate the effect of human economic activities on the biodiversity of ecosystem. However, the wide utilization of existing detection biosensors is limited by bulky and expensive instruments, such as Raman spectroscopy and electrochemical station. Herein, a liquid–solid triboelectric nanogenerator (TENG)‐based DNA barcode detection biosensor is proposed, which consists of water flow, fluid channel, and PDMS film attached by specifically designed capture probe. Through sequentially combining capture probe, targeted DNA barcode, and signal probe with Au nanoparticles (NPs), the surface charge density of friction layer of TENG decreases under the effect of AuNPs, verified by the density functional theory (DFT) method. Consequently, the peak value of output current spike signal for targeted DNA is smaller than that for other DNA, which is the working mechanism of the present TENG‐based biosensor. Such biosensor successfully recognizes Alvinocaris muricola among different types of Alvinocarididae shrimps, and its low limit detection can reach 1×10−12 m. The present work provides a paradigm‐shift way to develop an inexpensive and accurate technique to detect DNA barcode for species identification, and paves a novel way for the application of liquid–solid TENG. |
| format | Article |
| id | doaj-art-99c42d442952433da8077b92d42c08ee |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-99c42d442952433da8077b92d42c08ee2025-01-29T09:50:19ZengWileyAdvanced Science2198-38442025-01-01124n/an/a10.1002/advs.202408718Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species IdentificationWenlong Ma0Jiawei Li1Xiaolin Qu2Shao‘e Sun3Yanan Zhou4Yitong Liu5Peng Wang6Zhongli Sha7Key Laboratory of Advanced Marine Materials Key Laboratory of Marine Environmental Corrosion and Bio‐fouling Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaKey Laboratory of Advanced Marine Materials Key Laboratory of Marine Environmental Corrosion and Bio‐fouling Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaKey Laboratory of Advanced Marine Materials Key Laboratory of Marine Environmental Corrosion and Bio‐fouling Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaDepartment of Marine Organism Taxonomy & Phylogeny Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaKey Laboratory of Advanced Marine Materials Key Laboratory of Marine Environmental Corrosion and Bio‐fouling Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaKey Laboratory of Advanced Marine Materials Key Laboratory of Marine Environmental Corrosion and Bio‐fouling Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaKey Laboratory of Advanced Marine Materials Key Laboratory of Marine Environmental Corrosion and Bio‐fouling Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaDepartment of Marine Organism Taxonomy & Phylogeny Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 ChinaAbstract DNA barcode detection method is widely applied for species identification, which is imperative to evaluate the effect of human economic activities on the biodiversity of ecosystem. However, the wide utilization of existing detection biosensors is limited by bulky and expensive instruments, such as Raman spectroscopy and electrochemical station. Herein, a liquid–solid triboelectric nanogenerator (TENG)‐based DNA barcode detection biosensor is proposed, which consists of water flow, fluid channel, and PDMS film attached by specifically designed capture probe. Through sequentially combining capture probe, targeted DNA barcode, and signal probe with Au nanoparticles (NPs), the surface charge density of friction layer of TENG decreases under the effect of AuNPs, verified by the density functional theory (DFT) method. Consequently, the peak value of output current spike signal for targeted DNA is smaller than that for other DNA, which is the working mechanism of the present TENG‐based biosensor. Such biosensor successfully recognizes Alvinocaris muricola among different types of Alvinocarididae shrimps, and its low limit detection can reach 1×10−12 m. The present work provides a paradigm‐shift way to develop an inexpensive and accurate technique to detect DNA barcode for species identification, and paves a novel way for the application of liquid–solid TENG.https://doi.org/10.1002/advs.202408718biosensorDNA barcode detectionspecies identificationtriboelectric nanogenerator |
| spellingShingle | Wenlong Ma Jiawei Li Xiaolin Qu Shao‘e Sun Yanan Zhou Yitong Liu Peng Wang Zhongli Sha Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification Advanced Science biosensor DNA barcode detection species identification triboelectric nanogenerator |
| title | Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification |
| title_full | Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification |
| title_fullStr | Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification |
| title_full_unstemmed | Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification |
| title_short | Liquid–Solid Triboelectric Nanogenerator‐Based DNA Barcode Detection Biosensor for Species Identification |
| title_sort | liquid solid triboelectric nanogenerator based dna barcode detection biosensor for species identification |
| topic | biosensor DNA barcode detection species identification triboelectric nanogenerator |
| url | https://doi.org/10.1002/advs.202408718 |
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