A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis
A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/540240 |
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| author | Bareki Shima Batlokwa Janes Mokgadi Ronald Majors Charlotta Turner Nelson Torto |
| author_facet | Bareki Shima Batlokwa Janes Mokgadi Ronald Majors Charlotta Turner Nelson Torto |
| author_sort | Bareki Shima Batlokwa |
| collection | DOAJ |
| description | A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll a was synthesized by copolymerizing the functional and cross-linking monomers, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), respectively. During batch rebinding experiments, the MIP effectively removed chlorophyll from neat chlorophyll standards, green medicinal plants, and spinach extracts with determined absorbance as high as 2.501 absorbance units (Au) to as low as 0.084 Au after the optimum quantity of the MIP (170 mg mL−1) was added as the adsorbent prior to instrumental analysis. The determined 0.084 Au absorbance was far below the absorbance of 2% chlorophyll (0.401 Au) regarded as the cut-off point for interfering chlorophyll. Thus the newly developed polymer presents itself as a suitable adsorbent for the selective removal of chlorophyll from heavily pigmented, chlorophyll
containing extracts. |
| format | Article |
| id | doaj-art-999f258d642947a0b9a4d7d21d31fd66 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-999f258d642947a0b9a4d7d21d31fd662025-02-03T06:07:58ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/540240540240A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental AnalysisBareki Shima Batlokwa0Janes Mokgadi1Ronald Majors2Charlotta Turner3Nelson Torto4Department of Earth & Environmental Science (Chemistry Unit), Botswana International University of Science & Technology, P.O. Box 041, Bontleng, Gaborone, BotswanaDepartment of Chemistry, Rhodes University, P.O. Box 94, Grahamstown 6140, South AfricaAgilent Technologies, Inc., 2850 Centerville Road, Wilmington, DE 19808, USADepartment of Chemistry, Center for Analysis and Synthesis, Lund University, P.O. Box 124, 221 00 Lund, SwedenDepartment of Chemistry, Rhodes University, P.O. Box 94, Grahamstown 6140, South AfricaA novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll a was synthesized by copolymerizing the functional and cross-linking monomers, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), respectively. During batch rebinding experiments, the MIP effectively removed chlorophyll from neat chlorophyll standards, green medicinal plants, and spinach extracts with determined absorbance as high as 2.501 absorbance units (Au) to as low as 0.084 Au after the optimum quantity of the MIP (170 mg mL−1) was added as the adsorbent prior to instrumental analysis. The determined 0.084 Au absorbance was far below the absorbance of 2% chlorophyll (0.401 Au) regarded as the cut-off point for interfering chlorophyll. Thus the newly developed polymer presents itself as a suitable adsorbent for the selective removal of chlorophyll from heavily pigmented, chlorophyll containing extracts.http://dx.doi.org/10.1155/2013/540240 |
| spellingShingle | Bareki Shima Batlokwa Janes Mokgadi Ronald Majors Charlotta Turner Nelson Torto A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis Journal of Chemistry |
| title | A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis |
| title_full | A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis |
| title_fullStr | A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis |
| title_full_unstemmed | A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis |
| title_short | A Novel Molecularly Imprinted Polymer for the Selective Removal of Chlorophyll from Heavily Pigmented Green Plant Extracts prior to Instrumental Analysis |
| title_sort | novel molecularly imprinted polymer for the selective removal of chlorophyll from heavily pigmented green plant extracts prior to instrumental analysis |
| url | http://dx.doi.org/10.1155/2013/540240 |
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