The Increased Expression of HLA-DR and ICAM-1 Molecules by Human Bronchial Epithelial Cells, Induced by Activated Mononuclear Cells, is Downregulated by Nedocromil Sodium
To test the hypothesis that mononuclear cell products could increase the expression of HLA-DR and ICAM-1 molecules in bronchial epithelial cells (BECs), subconfluent cultures of human BECs, obtained from surgically resected bronchi, were incubated with PHA-activated blood mononuclear cell conditione...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000682 |
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| Summary: | To test the hypothesis that mononuclear cell products could increase
the expression of HLA-DR and ICAM-1 molecules in bronchial
epithelial cells (BECs), subconfluent cultures of human BECs,
obtained from surgically resected bronchi, were incubated with
PHA-activated blood mononuclear cell conditioned media (BCM-CM) or
recombinant IFN-γ. The presence of HLA-DR and ICAM-1 molecules
on BECs was then evaluated by specific antibody staining and
flow-cytometry analysis. The addition to BEC cultures of different
concentrations of PHA-stimulated BMC-CM, or of IFN-γ induced a
dosedependent increase of HIA-DR and ICAM-1 expression, while no
effect was observed with unstimulated BMC-CM. The ability of
nedocromil sodium and, as control, of dexamethasone, to prevent the
upregulation of HLA-DR and ICAM-1 expression on BECs was then
tested. Increasing concentrations (10−7 to 10−4 M) of nedocromil
significandy inhibited HLA-DR and ICAM-1 expression by BECs in a
dose-dependent fashion. A similarly dose-dependent inhibitory effect
was also observed with dexamethasone, which, however, was less
active than nedocromil on HL-ADR expression and more active on
ICAM-1 expression. Finally, nedocromil and dexamethasone showed a
significant synergistic effect on the expression of both cell
surface molecules at the lowest concentrations tested. |
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| ISSN: | 0962-9351 1466-1861 |