Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress
The high-glucose tolerance of yeast is the main factor determining the efficiency of high-density alcohol fermentation. Zygosaccharomyces mellis LGL-1 isolated from honey could survive under 700 g/L high-glucose stress and its tolerant characteristics were identified in our previous study. This stud...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2021-01-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302030149X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557322764812288 |
|---|---|
| author | Gongliang Liu Xinyu Bi Changli Tao Yongtao Fei Sujuan Gao Jinglong Liang Weidong Bai |
| author_facet | Gongliang Liu Xinyu Bi Changli Tao Yongtao Fei Sujuan Gao Jinglong Liang Weidong Bai |
| author_sort | Gongliang Liu |
| collection | DOAJ |
| description | The high-glucose tolerance of yeast is the main factor determining the efficiency of high-density alcohol fermentation. Zygosaccharomyces mellis LGL-1 isolated from honey could survive under 700 g/L high-glucose stress and its tolerant characteristics were identified in our previous study. This study was performed to explore and clarify the high-glucose tolerance mechanism of Z. mellis LGL-1. Comparative transcriptomic analysis was used to analyze the genes with differential expression in Z. mellis under high-glucose conditions of 300, 500 and 700 g/L. With 300 g/L samples as reference, there were 937 and 2380 differentially expressed genes (DEGs) in the 500 and 700 g/L samples, respectively. Meanwhile, there was 825 significant DEGs in the 700 g/L samples compared with that of the 500 g/L samples. The result revealed that transcriptional changes in multiple metabolic pathways occur in response to high-glucose stress. q-RT PCR analysis further confirmed that several stress response pathways, such as the high osmolarity glycerol mitogen-activated protein kinase (HOG-MAPK) signal transduction pathway, trehalose synthesis pathway and oxidative stress response are closely related to high-glucose tolerance in Z. mellis. This study clarifies mechanisms of Z. mellis in response to high-glucose osmotic stress, providing theoretical basis for the process control of high-density alcohol fermentation. |
| format | Article |
| id | doaj-art-993a70e32ed0476cb471eff6aa01125e |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-993a70e32ed0476cb471eff6aa01125e2025-02-03T05:21:09ZengTsinghua University PressFood Science and Human Wellness2213-45302021-01-011015462Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stressGongliang Liu0Xinyu Bi1Changli Tao2Yongtao Fei3Sujuan Gao4Jinglong Liang5Weidong Bai6College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR ChinaCollege of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR ChinaGuangdong Province Key Laboratory for Biotechnology Drug Candidates, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, PR ChinaCollege of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR China; Corresponding authors.College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR ChinaCollege of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR ChinaCollege of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, PR China; Corresponding authors.The high-glucose tolerance of yeast is the main factor determining the efficiency of high-density alcohol fermentation. Zygosaccharomyces mellis LGL-1 isolated from honey could survive under 700 g/L high-glucose stress and its tolerant characteristics were identified in our previous study. This study was performed to explore and clarify the high-glucose tolerance mechanism of Z. mellis LGL-1. Comparative transcriptomic analysis was used to analyze the genes with differential expression in Z. mellis under high-glucose conditions of 300, 500 and 700 g/L. With 300 g/L samples as reference, there were 937 and 2380 differentially expressed genes (DEGs) in the 500 and 700 g/L samples, respectively. Meanwhile, there was 825 significant DEGs in the 700 g/L samples compared with that of the 500 g/L samples. The result revealed that transcriptional changes in multiple metabolic pathways occur in response to high-glucose stress. q-RT PCR analysis further confirmed that several stress response pathways, such as the high osmolarity glycerol mitogen-activated protein kinase (HOG-MAPK) signal transduction pathway, trehalose synthesis pathway and oxidative stress response are closely related to high-glucose tolerance in Z. mellis. This study clarifies mechanisms of Z. mellis in response to high-glucose osmotic stress, providing theoretical basis for the process control of high-density alcohol fermentation.http://www.sciencedirect.com/science/article/pii/S221345302030149XZygosaccharomyces mellisTranscriptomicsHigh-glucose toleranceHOG-MAPKTrehalose |
| spellingShingle | Gongliang Liu Xinyu Bi Changli Tao Yongtao Fei Sujuan Gao Jinglong Liang Weidong Bai Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress Food Science and Human Wellness Zygosaccharomyces mellis Transcriptomics High-glucose tolerance HOG-MAPK Trehalose |
| title | Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress |
| title_full | Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress |
| title_fullStr | Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress |
| title_full_unstemmed | Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress |
| title_short | Comparative transcriptomics analysis of Zygosaccharomyces mellis under high-glucose stress |
| title_sort | comparative transcriptomics analysis of zygosaccharomyces mellis under high glucose stress |
| topic | Zygosaccharomyces mellis Transcriptomics High-glucose tolerance HOG-MAPK Trehalose |
| url | http://www.sciencedirect.com/science/article/pii/S221345302030149X |
| work_keys_str_mv | AT gongliangliu comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress AT xinyubi comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress AT changlitao comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress AT yongtaofei comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress AT sujuangao comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress AT jinglongliang comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress AT weidongbai comparativetranscriptomicsanalysisofzygosaccharomycesmellisunderhighglucosestress |