Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus
Two subtypes of the Crustacean Hyperglycemic Hormone (CHH) gene and the cDNA of Panulirus homarus (PhCHHA and PhCHH-B) were been successfully cloned and characterized in this study. The length of PhCHH-A was 1186 bp with a 384 bp open reading frame that encodes a 127 amino acid. The length of PhCHH-...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006409 |
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| author | Zhimin Ju Jiajun Luo Charles Brighton Ndandala Chongqing Wen Zhao Yanfei Huafang Liang |
| author_facet | Zhimin Ju Jiajun Luo Charles Brighton Ndandala Chongqing Wen Zhao Yanfei Huafang Liang |
| author_sort | Zhimin Ju |
| collection | DOAJ |
| description | Two subtypes of the Crustacean Hyperglycemic Hormone (CHH) gene and the cDNA of Panulirus homarus (PhCHHA and PhCHH-B) were been successfully cloned and characterized in this study. The length of PhCHH-A was 1186 bp with a 384 bp open reading frame that encodes a 127 amino acid. The length of PhCHH-B was 662 bp with a 394 bp open reading frame that encodes 131 amino acids. PhCHH-A and PhCHH-B possess most of the characteristics of eyestalk type-I CHH/MIH/GIH family neuropeptides. Both have a “signal peptide, CPRP, and mature peptide” structure. Their mature peptide comprised 74 amino acids and 6 cysteine residues in a conserved position. Homology and phylogenetic analysis showed that PhCHH-A and PhCHH-B were conserved in several functional domains. They showed the highest homology with CHH of Rock lobster (Jasus lalandii). Additionally, high levels of PhCHH-A transcripts were detected in both the intestine and eyestalks, where the expression of PhCHH-B was the highest in eyestalk. In female lobsters, PhCHH-A and PhCHH-B expression levelin eyestalk gradually increased with ovarian development. The photoperiod experiment results showed that ovarian development was optimal at 14 L:10D, and the expression levels of PhCHH-A and PhCHH-B in the eyestalk increased to the peak. In conclusion, these results indicate that PhCHH-A and PhCHH-B might play a critical role in the ovarian developmental cycle of P. homarus. In addition, a photoperiod of 14 L:10D had the most apparent effect on promoting the expression of PhCHH-A and PhCHH-B, and ovarian development. |
| format | Article |
| id | doaj-art-98f3e92b240940d0a840e1b84651299f |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-98f3e92b240940d0a840e1b84651299f2025-02-06T05:12:06ZengElsevierAquaculture Reports2352-51342025-03-0140102552Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarusZhimin Ju0Jiajun Luo1Charles Brighton Ndandala2Chongqing Wen3Zhao Yanfei4Huafang Liang5Fisheries College, Guangdong Ocean University, Zhanjiang, PR ChinaFisheries College, Guangdong Ocean University, Zhanjiang, PR ChinaFisheries College, Guangdong Ocean University, Zhanjiang, PR ChinaFisheries College, Guangdong Ocean University, Zhanjiang, PR ChinaAgro-Tech Extension Center of Guangdong Province, Guangzhou, PR ChinaFisheries College, Guangdong Ocean University, Zhanjiang, PR China; Corresponding author.Two subtypes of the Crustacean Hyperglycemic Hormone (CHH) gene and the cDNA of Panulirus homarus (PhCHHA and PhCHH-B) were been successfully cloned and characterized in this study. The length of PhCHH-A was 1186 bp with a 384 bp open reading frame that encodes a 127 amino acid. The length of PhCHH-B was 662 bp with a 394 bp open reading frame that encodes 131 amino acids. PhCHH-A and PhCHH-B possess most of the characteristics of eyestalk type-I CHH/MIH/GIH family neuropeptides. Both have a “signal peptide, CPRP, and mature peptide” structure. Their mature peptide comprised 74 amino acids and 6 cysteine residues in a conserved position. Homology and phylogenetic analysis showed that PhCHH-A and PhCHH-B were conserved in several functional domains. They showed the highest homology with CHH of Rock lobster (Jasus lalandii). Additionally, high levels of PhCHH-A transcripts were detected in both the intestine and eyestalks, where the expression of PhCHH-B was the highest in eyestalk. In female lobsters, PhCHH-A and PhCHH-B expression levelin eyestalk gradually increased with ovarian development. The photoperiod experiment results showed that ovarian development was optimal at 14 L:10D, and the expression levels of PhCHH-A and PhCHH-B in the eyestalk increased to the peak. In conclusion, these results indicate that PhCHH-A and PhCHH-B might play a critical role in the ovarian developmental cycle of P. homarus. In addition, a photoperiod of 14 L:10D had the most apparent effect on promoting the expression of PhCHH-A and PhCHH-B, and ovarian development.http://www.sciencedirect.com/science/article/pii/S2352513424006409Panulirus homarusCrustacean hyperglycemic hormoneGene cloneGene expressionPhotoperiod |
| spellingShingle | Zhimin Ju Jiajun Luo Charles Brighton Ndandala Chongqing Wen Zhao Yanfei Huafang Liang Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus Aquaculture Reports Panulirus homarus Crustacean hyperglycemic hormone Gene clone Gene expression Photoperiod |
| title | Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus |
| title_full | Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus |
| title_fullStr | Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus |
| title_full_unstemmed | Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus |
| title_short | Cloning, expression analysis, and the photoperiodic response of CHH gene in Panulirus homarus |
| title_sort | cloning expression analysis and the photoperiodic response of chh gene in panulirus homarus |
| topic | Panulirus homarus Crustacean hyperglycemic hormone Gene clone Gene expression Photoperiod |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006409 |
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