Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia
Weeds thrive in agricultural environments. However, in certain areas of the world, they are consumed by humans as food, and they can represent a source of valuable active ingredients of ethnomedical interest. In this study, three North African parasitic plants of the Orobanchaceae family, Cistanche...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2020-06-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://www.journalssystem.com/pjfns/Evaluation-of-the-antioxidant-and-cytotoxic-activities-on-cancer-cell-line-of-extracts,122040,0,2.html |
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| author | Imen Ben Attia Paolo Zucca Flaminia Cesare Marincola Mariella Nieddu Alessandra Piras Antonella Rosa Antonio Rescigno Mohamed Chaieb |
| author_facet | Imen Ben Attia Paolo Zucca Flaminia Cesare Marincola Mariella Nieddu Alessandra Piras Antonella Rosa Antonio Rescigno Mohamed Chaieb |
| author_sort | Imen Ben Attia |
| collection | DOAJ |
| description | Weeds thrive in agricultural environments. However, in certain areas of the world, they are consumed by humans as food, and they can represent a source of valuable active ingredients of ethnomedical interest. In this study, three North African parasitic plants of the Orobanchaceae family, Cistanche violacea, Orobanche crenata and Orobanche lavandulacea , normally considered as weeds, were studied to compare their biological activities. The antioxidant activity of hexane, ethyl acetate, acetone, methanol, and water extracts obtained by sequential maceration was estimated by DPPH, ABTS and FRAP assays. The metabolic profiles of aqueous extracts were studied by 1 H NMR spectroscopy. The oils extracted using hexane as solvent were analyzed by GC-FID and HPLC-DAD to characterize the fatty acid profile. The cytotoxic effect of the oil from the parasitic plants was also studied on B16F10 melanoma cancer cells. All extracts showed antioxidant activity and differences between extracts obtained using different solvents as well as for different plants have been highlighted. The highest total phenolic content was determined in O. crenata acetone extract (3.53±0.20 mmol GAE/g) and in O. lavandulacea methanol extract (2.29±0.02 mmol GAE/g). 1 H NMR spectroscopy revealed the presence of amino acids, organic acids and carbohydrates. The major fatty acids in the oils were 18:2 n -6, 16:0, 18:1 n -9, and 18:3 n -3. O. lavandulacea revealed to be a highly cytotoxic species for B16F10 melanoma cells. These data suggest that, despite the extreme climate conditions and the damage that can result, the studied desert parasitic plants can represent a novel and precious source of metabolites, with promising biological activities. |
| format | Article |
| id | doaj-art-98bab5357c7d400893c5215ea893da16 |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-98bab5357c7d400893c5215ea893da162025-02-02T05:23:19ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072020-06-0170325326310.31883/pjfns/122040122040Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in TunisiaImen Ben Attia0Paolo Zucca1Flaminia Cesare Marincola2Mariella Nieddu3Alessandra Piras4Antonella Rosa5Antonio Rescigno6Mohamed Chaieb7LEBIOMAT, Faculty of Sciences, University of Sfax, TunisiaDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato (CA), ItalyDepartment of Chemical and Geological Sciences, University of Cagliari, 09042 Monserrato (CA), ItalyDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato (CA), ItalyDepartment of Chemical and Geological Sciences, University of Cagliari, 09042 Monserrato (CA), ItalyDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato (CA), ItalyDepartment of Biomedical Sciences, University of Cagliari, 09042 Monserrato (CA), ItalyLEBIOMAT, Faculty of Sciences, University of Sfax, TunisiaWeeds thrive in agricultural environments. However, in certain areas of the world, they are consumed by humans as food, and they can represent a source of valuable active ingredients of ethnomedical interest. In this study, three North African parasitic plants of the Orobanchaceae family, Cistanche violacea, Orobanche crenata and Orobanche lavandulacea , normally considered as weeds, were studied to compare their biological activities. The antioxidant activity of hexane, ethyl acetate, acetone, methanol, and water extracts obtained by sequential maceration was estimated by DPPH, ABTS and FRAP assays. The metabolic profiles of aqueous extracts were studied by 1 H NMR spectroscopy. The oils extracted using hexane as solvent were analyzed by GC-FID and HPLC-DAD to characterize the fatty acid profile. The cytotoxic effect of the oil from the parasitic plants was also studied on B16F10 melanoma cancer cells. All extracts showed antioxidant activity and differences between extracts obtained using different solvents as well as for different plants have been highlighted. The highest total phenolic content was determined in O. crenata acetone extract (3.53±0.20 mmol GAE/g) and in O. lavandulacea methanol extract (2.29±0.02 mmol GAE/g). 1 H NMR spectroscopy revealed the presence of amino acids, organic acids and carbohydrates. The major fatty acids in the oils were 18:2 n -6, 16:0, 18:1 n -9, and 18:3 n -3. O. lavandulacea revealed to be a highly cytotoxic species for B16F10 melanoma cells. These data suggest that, despite the extreme climate conditions and the damage that can result, the studied desert parasitic plants can represent a novel and precious source of metabolites, with promising biological activities.http://www.journalssystem.com/pjfns/Evaluation-of-the-antioxidant-and-cytotoxic-activities-on-cancer-cell-line-of-extracts,122040,0,2.htmlweedsresourcewild ediblesbiodiversity fooddpphphenolics |
| spellingShingle | Imen Ben Attia Paolo Zucca Flaminia Cesare Marincola Mariella Nieddu Alessandra Piras Antonella Rosa Antonio Rescigno Mohamed Chaieb Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia Polish Journal of Food and Nutrition Sciences weeds resource wild edibles biodiversity food dpph phenolics |
| title | Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia |
| title_full | Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia |
| title_fullStr | Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia |
| title_full_unstemmed | Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia |
| title_short | Evaluation of the Antioxidant and Cytotoxic Activities on Cancer Cell Line of Extracts of Parasitic Plants Harvested in Tunisia |
| title_sort | evaluation of the antioxidant and cytotoxic activities on cancer cell line of extracts of parasitic plants harvested in tunisia |
| topic | weeds resource wild edibles biodiversity food dpph phenolics |
| url | http://www.journalssystem.com/pjfns/Evaluation-of-the-antioxidant-and-cytotoxic-activities-on-cancer-cell-line-of-extracts,122040,0,2.html |
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