A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields
Convective Polymerase Chain Reaction (cPCR), owing to its enhanced thermal cycling efficiency, holds promise for application in the next generation of mainstream commercial PCR instruments. Despite its potential, existing capillary-based and annular reaction chamber designs encounter limitations in...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Chemosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9040/13/1/6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832588850318278656 |
|---|---|
| author | Chenfei Li Yaping Xie Haochen Yong Xin Zhao Xingxing Ke Zhigang Wu |
| author_facet | Chenfei Li Yaping Xie Haochen Yong Xin Zhao Xingxing Ke Zhigang Wu |
| author_sort | Chenfei Li |
| collection | DOAJ |
| description | Convective Polymerase Chain Reaction (cPCR), owing to its enhanced thermal cycling efficiency, holds promise for application in the next generation of mainstream commercial PCR instruments. Despite its potential, existing capillary-based and annular reaction chamber designs encounter limitations in precisely controlling the internal flow field, which poses a significant barrier to the progression of cPCR. To overcome these obstacles, this work innovatively proposes a cPCR chip utilizing a “racetrack-shaped” reaction chamber, along with a reverse design approach tailored to meet diverse reaction requirements. Through modeling and simulation, we accurately obtained the relationship between the design parameters and the average flow velocity of the cPCR chip with a “racetrack-shaped” reaction chamber. By capturing the motion of fluorescent particles using a high-speed camera, we acquired the velocity distribution of the actual flow field. Further, we utilized these relationships to conduct a reverse design. Ultimately, a reaction chamber was designed based on the actual amplification needs of 2019-nCoV and hepatitis B virus, and successful amplification was achieved using a self-developed temperature control platform. |
| format | Article |
| id | doaj-art-98abc7e600ad4a01adc89f59657537a0 |
| institution | Kabale University |
| issn | 2227-9040 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj-art-98abc7e600ad4a01adc89f59657537a02025-01-24T13:26:52ZengMDPI AGChemosensors2227-90402025-01-01131610.3390/chemosensors13010006A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow FieldsChenfei Li0Yaping Xie1Haochen Yong2Xin Zhao3Xingxing Ke4Zhigang Wu5State Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaState Key Laboratory of Intelligent Manufacturing Equipment and Technology, School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, ChinaConvective Polymerase Chain Reaction (cPCR), owing to its enhanced thermal cycling efficiency, holds promise for application in the next generation of mainstream commercial PCR instruments. Despite its potential, existing capillary-based and annular reaction chamber designs encounter limitations in precisely controlling the internal flow field, which poses a significant barrier to the progression of cPCR. To overcome these obstacles, this work innovatively proposes a cPCR chip utilizing a “racetrack-shaped” reaction chamber, along with a reverse design approach tailored to meet diverse reaction requirements. Through modeling and simulation, we accurately obtained the relationship between the design parameters and the average flow velocity of the cPCR chip with a “racetrack-shaped” reaction chamber. By capturing the motion of fluorescent particles using a high-speed camera, we acquired the velocity distribution of the actual flow field. Further, we utilized these relationships to conduct a reverse design. Ultimately, a reaction chamber was designed based on the actual amplification needs of 2019-nCoV and hepatitis B virus, and successful amplification was achieved using a self-developed temperature control platform.https://www.mdpi.com/2227-9040/13/1/6microfluidic chipRayleigh–Bénard convectionconvective PCRreverse designflow field control |
| spellingShingle | Chenfei Li Yaping Xie Haochen Yong Xin Zhao Xingxing Ke Zhigang Wu A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields Chemosensors microfluidic chip Rayleigh–Bénard convection convective PCR reverse design flow field control |
| title | A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields |
| title_full | A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields |
| title_fullStr | A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields |
| title_full_unstemmed | A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields |
| title_short | A Reverse Design Method for Convective PCR Chips Featuring Precise Control of Steady-State Flow Fields |
| title_sort | reverse design method for convective pcr chips featuring precise control of steady state flow fields |
| topic | microfluidic chip Rayleigh–Bénard convection convective PCR reverse design flow field control |
| url | https://www.mdpi.com/2227-9040/13/1/6 |
| work_keys_str_mv | AT chenfeili areversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT yapingxie areversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT haochenyong areversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT xinzhao areversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT xingxingke areversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT zhigangwu areversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT chenfeili reversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT yapingxie reversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT haochenyong reversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT xinzhao reversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT xingxingke reversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields AT zhigangwu reversedesignmethodforconvectivepcrchipsfeaturingprecisecontrolofsteadystateflowfields |