Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice
Abstract Proteases are important modulators of inflammation, but they remain understudied in material‐induced immune response, which is critical to clinical success of biomedical implants. Herein, molecular expression and proteolytic activity of three distinct proteases, namely neutrophil elastase,...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-02-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202309709 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832540908822724608 |
|---|---|
| author | Nam M.P. Tran Anh T.H. Truong Dang T. Nguyen Tram T. Dang |
| author_facet | Nam M.P. Tran Anh T.H. Truong Dang T. Nguyen Tram T. Dang |
| author_sort | Nam M.P. Tran |
| collection | DOAJ |
| description | Abstract Proteases are important modulators of inflammation, but they remain understudied in material‐induced immune response, which is critical to clinical success of biomedical implants. Herein, molecular expression and proteolytic activity of three distinct proteases, namely neutrophil elastase, matrix metalloproteinases, cysteine cathepsins (cathepsin‐K and cathepsin‐B) are comprehensively profiled, in the subcutaneous host response of immuno‐competent mice against different biomaterial implants. Quantitative non‐invasive monitoring with activatable fluorescent probes reveals that different microparticulate materials induce distinct levels of protease activity with degradable poly(lactic‐co‐glycolic) acid inducing the strongest signal compared to nondegradable materials such as polystyrene and silica oxide. Furthermore, protein expression of selected proteases, attributable to both their inactive and active forms, notably deviates from their activities associated only with their active forms. Protease activity exhibits positive correlations with protein expression of pro‐inflammatory cytokines tumor necrosis factor α and interleukin 6 but negative correlation with pro‐fibrotic cytokine transforming growth factor β1. This study also demonstrates the predictive utility of protease activity as a non‐invasive, pro‐inflammatory parameter for evaluation of the anti‐inflammatory effects of model bioactive compounds on material‐induced host response. Overall, the findings provide new insights into protease presence in material‐induced immune responses, facilitating future biomaterial assessment to evoke appropriate host responses for implant applications. |
| format | Article |
| id | doaj-art-989dd2716aca4949abb9b0b3c16b70e9 |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-989dd2716aca4949abb9b0b3c16b70e92025-02-04T13:14:54ZengWileyAdvanced Science2198-38442025-02-01125n/an/a10.1002/advs.202309709Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent MiceNam M.P. Tran0Anh T.H. Truong1Dang T. Nguyen2Tram T. Dang3School of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University 70 Nanyang Drive Singapore 637459 SingaporeSchool of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University 70 Nanyang Drive Singapore 637459 SingaporeSchool of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University 70 Nanyang Drive Singapore 637459 SingaporeSchool of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University 70 Nanyang Drive Singapore 637459 SingaporeAbstract Proteases are important modulators of inflammation, but they remain understudied in material‐induced immune response, which is critical to clinical success of biomedical implants. Herein, molecular expression and proteolytic activity of three distinct proteases, namely neutrophil elastase, matrix metalloproteinases, cysteine cathepsins (cathepsin‐K and cathepsin‐B) are comprehensively profiled, in the subcutaneous host response of immuno‐competent mice against different biomaterial implants. Quantitative non‐invasive monitoring with activatable fluorescent probes reveals that different microparticulate materials induce distinct levels of protease activity with degradable poly(lactic‐co‐glycolic) acid inducing the strongest signal compared to nondegradable materials such as polystyrene and silica oxide. Furthermore, protein expression of selected proteases, attributable to both their inactive and active forms, notably deviates from their activities associated only with their active forms. Protease activity exhibits positive correlations with protein expression of pro‐inflammatory cytokines tumor necrosis factor α and interleukin 6 but negative correlation with pro‐fibrotic cytokine transforming growth factor β1. This study also demonstrates the predictive utility of protease activity as a non‐invasive, pro‐inflammatory parameter for evaluation of the anti‐inflammatory effects of model bioactive compounds on material‐induced host response. Overall, the findings provide new insights into protease presence in material‐induced immune responses, facilitating future biomaterial assessment to evoke appropriate host responses for implant applications.https://doi.org/10.1002/advs.202309709biomaterialhost immune responseinflammationproteasesubcutaneous implant |
| spellingShingle | Nam M.P. Tran Anh T.H. Truong Dang T. Nguyen Tram T. Dang Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice Advanced Science biomaterial host immune response inflammation protease subcutaneous implant |
| title | Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice |
| title_full | Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice |
| title_fullStr | Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice |
| title_full_unstemmed | Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice |
| title_short | Profiling Pro‐Inflammatory Proteases as Biomolecular Signatures of Material‐Induced Subcutaneous Host Response in Immuno‐Competent Mice |
| title_sort | profiling pro inflammatory proteases as biomolecular signatures of material induced subcutaneous host response in immuno competent mice |
| topic | biomaterial host immune response inflammation protease subcutaneous implant |
| url | https://doi.org/10.1002/advs.202309709 |
| work_keys_str_mv | AT nammptran profilingproinflammatoryproteasesasbiomolecularsignaturesofmaterialinducedsubcutaneoushostresponseinimmunocompetentmice AT anhthtruong profilingproinflammatoryproteasesasbiomolecularsignaturesofmaterialinducedsubcutaneoushostresponseinimmunocompetentmice AT dangtnguyen profilingproinflammatoryproteasesasbiomolecularsignaturesofmaterialinducedsubcutaneoushostresponseinimmunocompetentmice AT tramtdang profilingproinflammatoryproteasesasbiomolecularsignaturesofmaterialinducedsubcutaneoushostresponseinimmunocompetentmice |