Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability
Seed storability is a crucial agronomic trait and indispensable for the safe storage of rice seeds and grains. Nevertheless, the metabolite mechanisms governing <i>Indica</i> rice seed storability under natural conditions are still poorly understood. Methods: Therefore, the seed storage...
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2025-01-01
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| author | Fangxi Wu Yidong Wei Yongsheng Zhu Xi Luo Wei He Yingheng Wang Qiuhua Cai Huaan Xie Guosheng Xie Jianfu Zhang |
| author_facet | Fangxi Wu Yidong Wei Yongsheng Zhu Xi Luo Wei He Yingheng Wang Qiuhua Cai Huaan Xie Guosheng Xie Jianfu Zhang |
| author_sort | Fangxi Wu |
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| description | Seed storability is a crucial agronomic trait and indispensable for the safe storage of rice seeds and grains. Nevertheless, the metabolite mechanisms governing <i>Indica</i> rice seed storability under natural conditions are still poorly understood. Methods: Therefore, the seed storage tolerance of global rice core germplasms stored for two years under natural aging conditions were identified, and two extreme groups with different seed storabilities from the <i>Indica</i> rice group were analyzed using the UPLC-MS/MS metabolomic strategy. Results: Our results proved that the different rice core accessions showed significant variability in storage tolerance, and the metabolite analysis of the two <i>Indica</i> rice pools exhibited different levels of storability. A total of 103 differentially accumulated metabolites (DAMs) between the two pools were obtained, of which 38 were up-regulated and 65 were down-regulated, respectively. Further analysis disclosed that the aging-resistant rice accessions had higher accumulation levels of flavonoids, terpenoids, phenolic acids, organic acids, lignans, and coumarins while exhibiting lower levels of lipids and alkaloids compared to the storage-sensitive rice accessions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that several biosynthesis pathways were involved in the observed metabolite differences, including alpha-linolenic acid metabolism, butanoate metabolism, and propanoate metabolism. Notably, inhibition of the linolenic acid metabolic pathway could enhance seed storability. Additionally, increased accumulations of organic acids, such as succinic acid, D-malic acid, and methylmalonic acid, in the butanoate and propanoate metabolisms were identified as a beneficial factor for seed storage. Conclusions: These new findings will deepen our understanding of the underlying mechanisms governing rice storability. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Metabolites |
| spelling | doaj-art-986c60ba2a5e4da79907233a2d8da24e2025-01-24T13:41:11ZengMDPI AGMetabolites2218-19892025-01-011511910.3390/metabo15010019Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed StorabilityFangxi Wu0Yidong Wei1Yongsheng Zhu2Xi Luo3Wei He4Yingheng Wang5Qiuhua Cai6Huaan Xie7Guosheng Xie8Jianfu Zhang9Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaMinistry of Agriculture and Rural Affairs of the People’s Republic of China, Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaRice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350019, ChinaSeed storability is a crucial agronomic trait and indispensable for the safe storage of rice seeds and grains. Nevertheless, the metabolite mechanisms governing <i>Indica</i> rice seed storability under natural conditions are still poorly understood. Methods: Therefore, the seed storage tolerance of global rice core germplasms stored for two years under natural aging conditions were identified, and two extreme groups with different seed storabilities from the <i>Indica</i> rice group were analyzed using the UPLC-MS/MS metabolomic strategy. Results: Our results proved that the different rice core accessions showed significant variability in storage tolerance, and the metabolite analysis of the two <i>Indica</i> rice pools exhibited different levels of storability. A total of 103 differentially accumulated metabolites (DAMs) between the two pools were obtained, of which 38 were up-regulated and 65 were down-regulated, respectively. Further analysis disclosed that the aging-resistant rice accessions had higher accumulation levels of flavonoids, terpenoids, phenolic acids, organic acids, lignans, and coumarins while exhibiting lower levels of lipids and alkaloids compared to the storage-sensitive rice accessions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that several biosynthesis pathways were involved in the observed metabolite differences, including alpha-linolenic acid metabolism, butanoate metabolism, and propanoate metabolism. Notably, inhibition of the linolenic acid metabolic pathway could enhance seed storability. Additionally, increased accumulations of organic acids, such as succinic acid, D-malic acid, and methylmalonic acid, in the butanoate and propanoate metabolisms were identified as a beneficial factor for seed storage. Conclusions: These new findings will deepen our understanding of the underlying mechanisms governing rice storability.https://www.mdpi.com/2218-1989/15/1/19rice (<i>Oryza sativa</i> L.)seed storabilitymetabolomemetabolism pathway |
| spellingShingle | Fangxi Wu Yidong Wei Yongsheng Zhu Xi Luo Wei He Yingheng Wang Qiuhua Cai Huaan Xie Guosheng Xie Jianfu Zhang Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability Metabolites rice (<i>Oryza sativa</i> L.) seed storability metabolome metabolism pathway |
| title | Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability |
| title_full | Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability |
| title_fullStr | Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability |
| title_full_unstemmed | Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability |
| title_short | Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability |
| title_sort | comparative metabolic analysis of different i indica i rice varieties associated with seed storability |
| topic | rice (<i>Oryza sativa</i> L.) seed storability metabolome metabolism pathway |
| url | https://www.mdpi.com/2218-1989/15/1/19 |
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