Comparative Metabolic Analysis of Different <i>Indica</i> Rice Varieties Associated with Seed Storability
Seed storability is a crucial agronomic trait and indispensable for the safe storage of rice seeds and grains. Nevertheless, the metabolite mechanisms governing <i>Indica</i> rice seed storability under natural conditions are still poorly understood. Methods: Therefore, the seed storage...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
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| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/15/1/19 |
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| Summary: | Seed storability is a crucial agronomic trait and indispensable for the safe storage of rice seeds and grains. Nevertheless, the metabolite mechanisms governing <i>Indica</i> rice seed storability under natural conditions are still poorly understood. Methods: Therefore, the seed storage tolerance of global rice core germplasms stored for two years under natural aging conditions were identified, and two extreme groups with different seed storabilities from the <i>Indica</i> rice group were analyzed using the UPLC-MS/MS metabolomic strategy. Results: Our results proved that the different rice core accessions showed significant variability in storage tolerance, and the metabolite analysis of the two <i>Indica</i> rice pools exhibited different levels of storability. A total of 103 differentially accumulated metabolites (DAMs) between the two pools were obtained, of which 38 were up-regulated and 65 were down-regulated, respectively. Further analysis disclosed that the aging-resistant rice accessions had higher accumulation levels of flavonoids, terpenoids, phenolic acids, organic acids, lignans, and coumarins while exhibiting lower levels of lipids and alkaloids compared to the storage-sensitive rice accessions. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that several biosynthesis pathways were involved in the observed metabolite differences, including alpha-linolenic acid metabolism, butanoate metabolism, and propanoate metabolism. Notably, inhibition of the linolenic acid metabolic pathway could enhance seed storability. Additionally, increased accumulations of organic acids, such as succinic acid, D-malic acid, and methylmalonic acid, in the butanoate and propanoate metabolisms were identified as a beneficial factor for seed storage. Conclusions: These new findings will deepen our understanding of the underlying mechanisms governing rice storability. |
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| ISSN: | 2218-1989 |