Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism
In recent years, the use of organophosphorus pesticides has been extensively increased and these compounds signify a major class of agricultural pesticides today. We studied antigenotoxic potential of curcumin and carvacrol against the parathion induced DNA damage in cultured peripheral blood lympho...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2014/404236 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562828280594432 |
|---|---|
| author | Neeraj Kumar Anita Yadav Sachin Gulati Kanupriya Neeraj Aggarwal Ranjan Gupta |
| author_facet | Neeraj Kumar Anita Yadav Sachin Gulati Kanupriya Neeraj Aggarwal Ranjan Gupta |
| author_sort | Neeraj Kumar |
| collection | DOAJ |
| description | In recent years, the use of organophosphorus pesticides has been extensively increased and these compounds signify a major class of agricultural pesticides today. We studied antigenotoxic potential of curcumin and carvacrol against the parathion induced DNA damage in cultured peripheral blood lymphocytes using sister chromatid exchanges as a biomarker of genotoxicity. Heparinised fresh blood from healthy individuals was treated with 2.5 μg/mL concentration of parathion in presence of curcumin and carvacrol in order to observe the antigenotoxic potential of both curcumin and carvacrol. Significant reduction (P<0.05) was observed in the frequencies of SCEs in presence of 10 μg/mL and 15 μg/mL concentrations of curcumin as compared to parathion exposed sample. Similarly carvacrol had significant (P<0.05) antigenotoxic effect at the concentrations of 2.5 μg/mL and 5.0 μg/mL against the parathion. We also studied the effect of GSTT1 and GSTM1 on genotoxicity of parathion and antigenotoxic potential of curcumin and carvacrol. We did not observe any significant effect (P>0.05) of GSTT1 and GSTM1 polymorphism on genotoxicity of parathion and antigenotoxic potential of curcumin and carvacrol. |
| format | Article |
| id | doaj-art-9856383ef2ee46afa47b8b3aee6332bf |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-9856383ef2ee46afa47b8b3aee6332bf2025-02-03T01:21:43ZengWileyJournal of Toxicology1687-81911687-82052014-01-01201410.1155/2014/404236404236Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 PolymorphismNeeraj Kumar0Anita Yadav1Sachin Gulati2Kanupriya3Neeraj Aggarwal4Ranjan Gupta5Department of Biotechnology, Kurukshetra University Kurukshetra, Haryana 136119, IndiaDepartment of Biotechnology, Kurukshetra University Kurukshetra, Haryana 136119, IndiaDepartment of Biotechnology, Kurukshetra University Kurukshetra, Haryana 136119, IndiaDepartment of Biotechnology, Kurukshetra University Kurukshetra, Haryana 136119, IndiaDepartment of Microbiology, Kurukshetra University Kurukshetra, Haryana 136119, IndiaDepartment of Biochemistry, Kurukshetra University Kurukshetra, Haryana 136119, IndiaIn recent years, the use of organophosphorus pesticides has been extensively increased and these compounds signify a major class of agricultural pesticides today. We studied antigenotoxic potential of curcumin and carvacrol against the parathion induced DNA damage in cultured peripheral blood lymphocytes using sister chromatid exchanges as a biomarker of genotoxicity. Heparinised fresh blood from healthy individuals was treated with 2.5 μg/mL concentration of parathion in presence of curcumin and carvacrol in order to observe the antigenotoxic potential of both curcumin and carvacrol. Significant reduction (P<0.05) was observed in the frequencies of SCEs in presence of 10 μg/mL and 15 μg/mL concentrations of curcumin as compared to parathion exposed sample. Similarly carvacrol had significant (P<0.05) antigenotoxic effect at the concentrations of 2.5 μg/mL and 5.0 μg/mL against the parathion. We also studied the effect of GSTT1 and GSTM1 on genotoxicity of parathion and antigenotoxic potential of curcumin and carvacrol. We did not observe any significant effect (P>0.05) of GSTT1 and GSTM1 polymorphism on genotoxicity of parathion and antigenotoxic potential of curcumin and carvacrol.http://dx.doi.org/10.1155/2014/404236 |
| spellingShingle | Neeraj Kumar Anita Yadav Sachin Gulati Kanupriya Neeraj Aggarwal Ranjan Gupta Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism Journal of Toxicology |
| title | Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism |
| title_full | Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism |
| title_fullStr | Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism |
| title_full_unstemmed | Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism |
| title_short | Antigenotoxic Effect of Curcumin and Carvacrol against Parathion Induced DNA Damage in Cultured Human Peripheral Blood Lymphocytes and Its Relation to GSTM1 and GSTT1 Polymorphism |
| title_sort | antigenotoxic effect of curcumin and carvacrol against parathion induced dna damage in cultured human peripheral blood lymphocytes and its relation to gstm1 and gstt1 polymorphism |
| url | http://dx.doi.org/10.1155/2014/404236 |
| work_keys_str_mv | AT neerajkumar antigenotoxiceffectofcurcuminandcarvacrolagainstparathioninduceddnadamageinculturedhumanperipheralbloodlymphocytesanditsrelationtogstm1andgstt1polymorphism AT anitayadav antigenotoxiceffectofcurcuminandcarvacrolagainstparathioninduceddnadamageinculturedhumanperipheralbloodlymphocytesanditsrelationtogstm1andgstt1polymorphism AT sachingulati antigenotoxiceffectofcurcuminandcarvacrolagainstparathioninduceddnadamageinculturedhumanperipheralbloodlymphocytesanditsrelationtogstm1andgstt1polymorphism AT kanupriya antigenotoxiceffectofcurcuminandcarvacrolagainstparathioninduceddnadamageinculturedhumanperipheralbloodlymphocytesanditsrelationtogstm1andgstt1polymorphism AT neerajaggarwal antigenotoxiceffectofcurcuminandcarvacrolagainstparathioninduceddnadamageinculturedhumanperipheralbloodlymphocytesanditsrelationtogstm1andgstt1polymorphism AT ranjangupta antigenotoxiceffectofcurcuminandcarvacrolagainstparathioninduceddnadamageinculturedhumanperipheralbloodlymphocytesanditsrelationtogstm1andgstt1polymorphism |