Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells
The present study is designed to find out if sesquiterpenes, α-humulene (HUM), valencene (VAL), β-caryphyllene-oxide (CAO) and trans-nerolidol (NER), are able to improve the antiproliferative effect of classical cytostatic drugs, 5-fluorouracil (FU) and oxaliplatin (1,2-diaminocyclohexaneoxalato-pla...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2019-03-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2019-0003 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832573775430811648 |
|---|---|
| author | Ambrož Martin Šmatová Markéta Šadibolová Michaela Pospíšilová Eva Hadravská Pavlína Kašparová Michaela Skarková Veronika Hanušová Králová Věra Skálová Lenka |
| author_facet | Ambrož Martin Šmatová Markéta Šadibolová Michaela Pospíšilová Eva Hadravská Pavlína Kašparová Michaela Skarková Veronika Hanušová Králová Věra Skálová Lenka |
| author_sort | Ambrož Martin |
| collection | DOAJ |
| description | The present study is designed to find out if sesquiterpenes, α-humulene (HUM), valencene (VAL), β-caryphyllene-oxide (CAO) and trans-nerolidol (NER), are able to improve the antiproliferative effect of classical cytostatic drugs, 5-fluorouracil (FU) and oxaliplatin (1,2-diaminocyclohexaneoxalato-platinum, OxPt), in colon cancer cell lines Caco-2 and SW-620. In addition, the possible mechanisms of sesquiterpene action are studied. The results show significant ability of HUM and especially of CAO to enhance the anti-proliferative effects of FU and OxPt in cancer cell lines Caco-2 and SW-620. On the other hand, VAL and NER are ineffective. The action of CAO could be partly based on its ability to disrupt the mitochondrial membrane potential and to activate initiator caspases, but other mechanisms are probably also involved. Based on these results, CAO seems to have the potential for combination therapy of colon cancers and deserves further study. |
| format | Article |
| id | doaj-art-984dafe5b5f846dc8a00a5f9063c1539 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2019-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-984dafe5b5f846dc8a00a5f9063c15392025-02-02T02:50:42ZengSciendoActa Pharmaceutica1846-95582019-03-0169112112810.2478/acph-2019-0003acph-2019-0003Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cellsAmbrož Martin0Šmatová Markéta1Šadibolová Michaela2Pospíšilová Eva3Hadravská Pavlína4Kašparová Michaela5Skarková Veronika Hanušová6Králová Věra7Skálová Lenka8Department of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicDepartment of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicDepartment of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicDepartment of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicDepartment of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicDepartment of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicDepartment of Medical Biology and Genetics, Charles University in PragueFaculty of Medicine in Hradec Králové Šimkova 870, Hradec KrálovéCZ-500 38, Czech RepublicDepartment of Medical Biology and Genetics, Charles University in PragueFaculty of Medicine in Hradec Králové Šimkova 870, Hradec KrálovéCZ-500 38, Czech RepublicDepartment of Biochemical SciencesCharles University in PragueFaculty of Pharmacy, Heyrovského 1203, Hradec Králové, CZ-500 05 Czech RepublicThe present study is designed to find out if sesquiterpenes, α-humulene (HUM), valencene (VAL), β-caryphyllene-oxide (CAO) and trans-nerolidol (NER), are able to improve the antiproliferative effect of classical cytostatic drugs, 5-fluorouracil (FU) and oxaliplatin (1,2-diaminocyclohexaneoxalato-platinum, OxPt), in colon cancer cell lines Caco-2 and SW-620. In addition, the possible mechanisms of sesquiterpene action are studied. The results show significant ability of HUM and especially of CAO to enhance the anti-proliferative effects of FU and OxPt in cancer cell lines Caco-2 and SW-620. On the other hand, VAL and NER are ineffective. The action of CAO could be partly based on its ability to disrupt the mitochondrial membrane potential and to activate initiator caspases, but other mechanisms are probably also involved. Based on these results, CAO seems to have the potential for combination therapy of colon cancers and deserves further study.https://doi.org/10.2478/acph-2019-0003terpenescaco-2sw-620combination therapy |
| spellingShingle | Ambrož Martin Šmatová Markéta Šadibolová Michaela Pospíšilová Eva Hadravská Pavlína Kašparová Michaela Skarková Veronika Hanušová Králová Věra Skálová Lenka Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells Acta Pharmaceutica terpenes caco-2 sw-620 combination therapy |
| title | Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells |
| title_full | Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells |
| title_fullStr | Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells |
| title_full_unstemmed | Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells |
| title_short | Sesquiterpenes α-humulene and β-caryophyllene oxide enhance the efficacy of 5-fluorouracil and oxaliplatin in colon cancer cells |
| title_sort | sesquiterpenes α humulene and β caryophyllene oxide enhance the efficacy of 5 fluorouracil and oxaliplatin in colon cancer cells |
| topic | terpenes caco-2 sw-620 combination therapy |
| url | https://doi.org/10.2478/acph-2019-0003 |
| work_keys_str_mv | AT ambrozmartin sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT smatovamarketa sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT sadibolovamichaela sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT pospisilovaeva sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT hadravskapavlina sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT kasparovamichaela sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT skarkovaveronikahanusova sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT kralovavera sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells AT skalovalenka sesquiterpenesahumuleneandbcaryophylleneoxideenhancetheefficacyof5fluorouracilandoxaliplatinincoloncancercells |