Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production
Alkaline protease is one of the most important industrial enzymes which are excessively used in the detergent industry, food industry, feed industry, pharmaceutical industry, leather industry, etc. 60% of the produced alkaline protease is consumed by the detergent industry alone. In the present stud...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/6831092 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568159357370368 |
|---|---|
| author | Gyanu Raj Pandey Asmita Shrestha Tika B. Karki Shardul Neupane Sabnam Ojha Prashanna Koirala Parash Mani Timilsina |
| author_facet | Gyanu Raj Pandey Asmita Shrestha Tika B. Karki Shardul Neupane Sabnam Ojha Prashanna Koirala Parash Mani Timilsina |
| author_sort | Gyanu Raj Pandey |
| collection | DOAJ |
| description | Alkaline protease is one of the most important industrial enzymes which are excessively used in the detergent industry, food industry, feed industry, pharmaceutical industry, leather industry, etc. 60% of the produced alkaline protease is consumed by the detergent industry alone. In the present study, bacterial isolates that can produce alkaline protease for purpose of bio-detergent were screened among the isolates isolated from kinema (an alkaline fermented food of eastern Nepal). Selected bacterial isolates were further screened for hemolysis activity and the production of other hydrolytic enzymes. Four bacterial isolates selected were tested for their capacity to produce alkaline protease in five different fermentation mediums. Isolate BKHE produces a high amount of alkaline protease (0.4705 ± 0.035 U/mL/min) in fermentation medium M2 (sucrose, 11 g/L; yeast extract, 5 g/L; and KNO3, 5.2 g/l, pH 9). The selected isolate was identified as Bacillus amyloliquefaciens BKHE based on 16S rRNA sequencing and phenotypic features. This bacterial strain was also found to be thermotolerant (confluent growth at 50°C) and salt tolerant up to 10% NaCl concentration. With its versatile ability, bacterial isolate or purified enzymes have potential applications in the food and detergent industry. |
| format | Article |
| id | doaj-art-98433d840d0b4dffa56a3843a2b8f3d1 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-98433d840d0b4dffa56a3843a2b8f3d12025-02-03T00:59:37ZengWileyInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/6831092Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease ProductionGyanu Raj Pandey0Asmita Shrestha1Tika B. Karki2Shardul Neupane3Sabnam Ojha4Prashanna Koirala5Parash Mani Timilsina6Biotechnological Research and Development Center Pvt. LtdBiotechnological Research and Development Center Pvt. LtdDepartment of BiotechnologyDepartment of BiotechnologyDepartment of BiotechnologyDepartment of BiotechnologyDepartment of BiotechnologyAlkaline protease is one of the most important industrial enzymes which are excessively used in the detergent industry, food industry, feed industry, pharmaceutical industry, leather industry, etc. 60% of the produced alkaline protease is consumed by the detergent industry alone. In the present study, bacterial isolates that can produce alkaline protease for purpose of bio-detergent were screened among the isolates isolated from kinema (an alkaline fermented food of eastern Nepal). Selected bacterial isolates were further screened for hemolysis activity and the production of other hydrolytic enzymes. Four bacterial isolates selected were tested for their capacity to produce alkaline protease in five different fermentation mediums. Isolate BKHE produces a high amount of alkaline protease (0.4705 ± 0.035 U/mL/min) in fermentation medium M2 (sucrose, 11 g/L; yeast extract, 5 g/L; and KNO3, 5.2 g/l, pH 9). The selected isolate was identified as Bacillus amyloliquefaciens BKHE based on 16S rRNA sequencing and phenotypic features. This bacterial strain was also found to be thermotolerant (confluent growth at 50°C) and salt tolerant up to 10% NaCl concentration. With its versatile ability, bacterial isolate or purified enzymes have potential applications in the food and detergent industry.http://dx.doi.org/10.1155/2022/6831092 |
| spellingShingle | Gyanu Raj Pandey Asmita Shrestha Tika B. Karki Shardul Neupane Sabnam Ojha Prashanna Koirala Parash Mani Timilsina Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production International Journal of Microbiology |
| title | Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production |
| title_full | Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production |
| title_fullStr | Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production |
| title_full_unstemmed | Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production |
| title_short | Screening and Identification of Thermotolerant and Osmotolerant Bacillus amyloliquefaciens BKHE Isolated from Kinema of Eastern Nepal for Alkaline Protease Production |
| title_sort | screening and identification of thermotolerant and osmotolerant bacillus amyloliquefaciens bkhe isolated from kinema of eastern nepal for alkaline protease production |
| url | http://dx.doi.org/10.1155/2022/6831092 |
| work_keys_str_mv | AT gyanurajpandey screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction AT asmitashrestha screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction AT tikabkarki screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction AT shardulneupane screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction AT sabnamojha screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction AT prashannakoirala screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction AT parashmanitimilsina screeningandidentificationofthermotolerantandosmotolerantbacillusamyloliquefaciensbkheisolatedfromkinemaofeasternnepalforalkalineproteaseproduction |