Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene
Improving the nutritional value of grain sorghum, a drought- and heat-tolerant grain crop, is an important task in the context of global warming. One of the reasons for the low nutritional value of sorghum grain is the resistance of its storage proteins (kafirins) to proteolytic digestion, which is...
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| Format: | Article |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/4056 |
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| author | L. A. Elkonin N. V. Borisenko T. E. Pylaev O. A. Kenzhegulov S. Kh. Sarsenova N. Yu. Selivanov V. M. Panin |
| author_facet | L. A. Elkonin N. V. Borisenko T. E. Pylaev O. A. Kenzhegulov S. Kh. Sarsenova N. Yu. Selivanov V. M. Panin |
| author_sort | L. A. Elkonin |
| collection | DOAJ |
| description | Improving the nutritional value of grain sorghum, a drought- and heat-tolerant grain crop, is an important task in the context of global warming. One of the reasons for the low nutritional value of sorghum grain is the resistance of its storage proteins (kafirins) to proteolytic digestion, which is due, among other things, to the structural organization of protein bodies, in which γ-kafirin, the most resistant to proteases, is located on the periphery, encapsulating more easily digested α-kafirins. The introduction of genetic constructs capable of inducing RNA silencing of the γ-kafirin (gKAF1) gene opens up prospects for solving this problem. Using Agrobacterium-mediated genetic transformation of immature embryos of the grain sorghum cv. Avans we have obtained a mutant with improved digestibility of endosperm proteins (up to 92 %) carrying a genetic construct for RNA silencing of the gKAF1 gene. The goal of this work was to study the stability of inheritance of the introduced genetic construct in T2–T4 generations, to identify the number of its copies, as well as to trace the manifestation of agronomically valuable traits in the offspring of the mutant. The mutant lines were grown in experimental plots in three randomized blocks. The studied lines were characterized by improved digestibility of kafirins, a modified type of endosperm, completely or partially devoid of the vitreous layer, an increased percentage of lysine (by 75 %), reduced plant height, peduncle length, 1000-grains weight, and grain yield from the panicle. In T2, a line with monogenic control of GA resistance was selected. qPCR analysis showed that in different T3 and T4 plants, the genetic construct was present in 2–4 copies. In T3, a line with a high digestibility of endosperm proteins (81 %) and a minimal decrease in agronomically valuable traits (by 5–7 %) was selected. |
| format | Article |
| id | doaj-art-981ca71a5055499eaa8b32067bd12c83 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-981ca71a5055499eaa8b32067bd12c832025-02-01T09:58:13ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-03-01281637310.18699/vjgb-24-081440Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin geneL. A. Elkonin0N. V. Borisenko1T. E. Pylaev2O. A. Kenzhegulov3S. Kh. Sarsenova4N. Yu. Selivanov5V. M. Panin6Federal Center of Agriculture Research of the South-East RegionFederal Center of Agriculture Research of the South-East RegionInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Federal Scientific Center of the Russian Academy of Sciences; Saratov State Medical University named after V.I. RazumovskyFederal Center of Agriculture Research of the South-East RegionFederal Center of Agriculture Research of the South-East RegionInstitute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Federal Scientific Center of the Russian Academy of SciencesFederal Center of Agriculture Research of the South-East RegionImproving the nutritional value of grain sorghum, a drought- and heat-tolerant grain crop, is an important task in the context of global warming. One of the reasons for the low nutritional value of sorghum grain is the resistance of its storage proteins (kafirins) to proteolytic digestion, which is due, among other things, to the structural organization of protein bodies, in which γ-kafirin, the most resistant to proteases, is located on the periphery, encapsulating more easily digested α-kafirins. The introduction of genetic constructs capable of inducing RNA silencing of the γ-kafirin (gKAF1) gene opens up prospects for solving this problem. Using Agrobacterium-mediated genetic transformation of immature embryos of the grain sorghum cv. Avans we have obtained a mutant with improved digestibility of endosperm proteins (up to 92 %) carrying a genetic construct for RNA silencing of the gKAF1 gene. The goal of this work was to study the stability of inheritance of the introduced genetic construct in T2–T4 generations, to identify the number of its copies, as well as to trace the manifestation of agronomically valuable traits in the offspring of the mutant. The mutant lines were grown in experimental plots in three randomized blocks. The studied lines were characterized by improved digestibility of kafirins, a modified type of endosperm, completely or partially devoid of the vitreous layer, an increased percentage of lysine (by 75 %), reduced plant height, peduncle length, 1000-grains weight, and grain yield from the panicle. In T2, a line with monogenic control of GA resistance was selected. qPCR analysis showed that in different T3 and T4 plants, the genetic construct was present in 2–4 copies. In T3, a line with a high digestibility of endosperm proteins (81 %) and a minimal decrease in agronomically valuable traits (by 5–7 %) was selected.https://vavilov.elpub.ru/jour/article/view/4056<i>sorghum bicolor</i>transgenic plantsrna silencingkafirinsqpcrgrain qualityendosperm texture |
| spellingShingle | L. A. Elkonin N. V. Borisenko T. E. Pylaev O. A. Kenzhegulov S. Kh. Sarsenova N. Yu. Selivanov V. M. Panin Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene Вавиловский журнал генетики и селекции <i>sorghum bicolor</i> transgenic plants rna silencing kafirins qpcr grain quality endosperm texture |
| title | Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene |
| title_full | Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene |
| title_fullStr | Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene |
| title_full_unstemmed | Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene |
| title_short | Manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for RNA silencing of the γ-kafirin gene |
| title_sort | manifestation of agronomically valuable traits in the progeny of a sorghum mutant carrying the genetic construct for rna silencing of the γ kafirin gene |
| topic | <i>sorghum bicolor</i> transgenic plants rna silencing kafirins qpcr grain quality endosperm texture |
| url | https://vavilov.elpub.ru/jour/article/view/4056 |
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