Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value
Background. Early detection of the SARS-CoV-2 is crucial for both the improvement of turnaround time and limiting the spread of the virus in the community. Thus, this study aims to establish rapid antigen tests as an effective diagnostic tool to improve the testing strategies of COVID-19 diagnosis....
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2022/9245248 |
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| author | Dipendra Kumar Mandal Bibek Raj Bhattarai Sushant Pokhrel Mandira Chhusyabaga Parmananda Bhandari Mahendra Prasad Bhatt Sujan Babu Marhattha |
| author_facet | Dipendra Kumar Mandal Bibek Raj Bhattarai Sushant Pokhrel Mandira Chhusyabaga Parmananda Bhandari Mahendra Prasad Bhatt Sujan Babu Marhattha |
| author_sort | Dipendra Kumar Mandal |
| collection | DOAJ |
| description | Background. Early detection of the SARS-CoV-2 is crucial for both the improvement of turnaround time and limiting the spread of the virus in the community. Thus, this study aims to establish rapid antigen tests as an effective diagnostic tool to improve the testing strategies of COVID-19 diagnosis. Methods. A laboratory based cross-sectional study was performed on the patients that visited Sukraraj Tropical and Infectious Disease Hospital (STIDH) in Kathmandu, Nepal, from November 2020 to January 2021. A total of 213 nasopharyngeal swabs were collected from both symptomatic and asymptomatic patients for rapid antigen test, followed by RT-PCR assay as reference test for confirmation of COVID-19. A standard questionnaire was administered to collect other information from patients. Data were collected and analyzed using SPSS version 20. Results. Out of 213 individuals, 75 tested positive in Ag-RDT test, while 118 tested positive for SARS-CoV-2 RNA genome via Real time PCR assay. The overall diagnostic performance of Ag-RDT showed 63.6% sensitivity and 97.9% specificity. The diagnostic accuracy of Ag- RDT was 78.9% with κ value 0.590, showing moderate agreement with RT-PCR. Significant difference (p value <0.001) was observed between Ag- RDT+ and Ag- RDT− results when compared to Cq values obtained from RT- PCR. Conclusion. The promising performance of Ag-RDT renders it useful as screening tool alongside RT-PCR to reduce transmission via improving contact tracing, implementation of local mitigation strategies, and refining existing testing protocol for diagnosis of COVID-19. |
| format | Article |
| id | doaj-art-98159a3c1218466e8bcea86889819339 |
| institution | Kabale University |
| issn | 1687-8647 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-98159a3c1218466e8bcea868898193392025-02-03T01:08:46ZengWileyAdvances in Virology1687-86472022-01-01202210.1155/2022/9245248Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq ValueDipendra Kumar Mandal0Bibek Raj Bhattarai1Sushant Pokhrel2Mandira Chhusyabaga3Parmananda Bhandari4Mahendra Prasad Bhatt5Sujan Babu Marhattha6Department of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineDepartment of Laboratory MedicineSukraraj Tropical and Infectious Disease HospitalDepartment of Laboratory MedicineDepartment of Laboratory MedicineBackground. Early detection of the SARS-CoV-2 is crucial for both the improvement of turnaround time and limiting the spread of the virus in the community. Thus, this study aims to establish rapid antigen tests as an effective diagnostic tool to improve the testing strategies of COVID-19 diagnosis. Methods. A laboratory based cross-sectional study was performed on the patients that visited Sukraraj Tropical and Infectious Disease Hospital (STIDH) in Kathmandu, Nepal, from November 2020 to January 2021. A total of 213 nasopharyngeal swabs were collected from both symptomatic and asymptomatic patients for rapid antigen test, followed by RT-PCR assay as reference test for confirmation of COVID-19. A standard questionnaire was administered to collect other information from patients. Data were collected and analyzed using SPSS version 20. Results. Out of 213 individuals, 75 tested positive in Ag-RDT test, while 118 tested positive for SARS-CoV-2 RNA genome via Real time PCR assay. The overall diagnostic performance of Ag-RDT showed 63.6% sensitivity and 97.9% specificity. The diagnostic accuracy of Ag- RDT was 78.9% with κ value 0.590, showing moderate agreement with RT-PCR. Significant difference (p value <0.001) was observed between Ag- RDT+ and Ag- RDT− results when compared to Cq values obtained from RT- PCR. Conclusion. The promising performance of Ag-RDT renders it useful as screening tool alongside RT-PCR to reduce transmission via improving contact tracing, implementation of local mitigation strategies, and refining existing testing protocol for diagnosis of COVID-19.http://dx.doi.org/10.1155/2022/9245248 |
| spellingShingle | Dipendra Kumar Mandal Bibek Raj Bhattarai Sushant Pokhrel Mandira Chhusyabaga Parmananda Bhandari Mahendra Prasad Bhatt Sujan Babu Marhattha Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value Advances in Virology |
| title | Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value |
| title_full | Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value |
| title_fullStr | Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value |
| title_full_unstemmed | Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value |
| title_short | Diagnostic Performance of SARS-CoV-2 Rapid Antigen Test in relation to RT-PCR Cq Value |
| title_sort | diagnostic performance of sars cov 2 rapid antigen test in relation to rt pcr cq value |
| url | http://dx.doi.org/10.1155/2022/9245248 |
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