A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation
Extracorporeal circulation (ECC) is necessary for conventional cardiac surgery and life support, but it often triggers systemic inflammation that can significantly damage tissue. Studies of ECC have been limited to large animals because of the complexity of the surgical procedures involved, which ha...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/412319 |
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| _version_ | 1832556003418177536 |
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| author | Shuhua Luo Menglin Tang Lei Du Lina Gong Jin Xu Youwen Chen Yabo Wang Ke Lin Qi An |
| author_facet | Shuhua Luo Menglin Tang Lei Du Lina Gong Jin Xu Youwen Chen Yabo Wang Ke Lin Qi An |
| author_sort | Shuhua Luo |
| collection | DOAJ |
| description | Extracorporeal circulation (ECC) is necessary for conventional cardiac surgery and life support, but it often triggers systemic inflammation that can significantly damage tissue. Studies of ECC have been limited to large animals because of the complexity of the surgical procedures involved, which has hampered detailed understanding of ECC-induced injury. Here we describe a minimally invasive mouse model of ECC that may allow more extensive mechanistic studies. The right carotid artery and external jugular vein of anesthetized adult male C57BL/6 mice were cannulated to allow blood flow through a 1/32-inch external tube. All animals (n=20) survived 30 min ECC and subsequent 60 min observation. Blood analysis after ECC showed significant increases in levels of tumor necrosis factor α, interleukin-6, and neutrophil elastase in plasma, lung, and renal tissues, as well as increases in plasma creatinine and cystatin C and decreases in the oxygenation index. Histopathology showed that ECC induced the expected lung inflammation, which included alveolar congestion, hemorrhage, neutrophil infiltration, and alveolar wall thickening; in renal tissue, ECC induced intracytoplasmic vacuolization, acute tubular necrosis, and epithelial swelling. Our results suggest that this novel, minimally invasive mouse model can recapitulate many of the clinical features of ECC-induced systemic inflammatory response and organ injury. |
| format | Article |
| id | doaj-art-98071ed13d0b41d2ab4e4663362069bc |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-98071ed13d0b41d2ab4e4663362069bc2025-02-03T05:46:39ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/412319412319A Novel Minimal Invasive Mouse Model of Extracorporeal CirculationShuhua Luo0Menglin Tang1Lei Du2Lina Gong3Jin Xu4Youwen Chen5Yabo Wang6Ke Lin7Qi An8Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaIntensive Care Unit, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Anesthesiology and Translational Neuroscience Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Experimental Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaDepartment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, ChinaExtracorporeal circulation (ECC) is necessary for conventional cardiac surgery and life support, but it often triggers systemic inflammation that can significantly damage tissue. Studies of ECC have been limited to large animals because of the complexity of the surgical procedures involved, which has hampered detailed understanding of ECC-induced injury. Here we describe a minimally invasive mouse model of ECC that may allow more extensive mechanistic studies. The right carotid artery and external jugular vein of anesthetized adult male C57BL/6 mice were cannulated to allow blood flow through a 1/32-inch external tube. All animals (n=20) survived 30 min ECC and subsequent 60 min observation. Blood analysis after ECC showed significant increases in levels of tumor necrosis factor α, interleukin-6, and neutrophil elastase in plasma, lung, and renal tissues, as well as increases in plasma creatinine and cystatin C and decreases in the oxygenation index. Histopathology showed that ECC induced the expected lung inflammation, which included alveolar congestion, hemorrhage, neutrophil infiltration, and alveolar wall thickening; in renal tissue, ECC induced intracytoplasmic vacuolization, acute tubular necrosis, and epithelial swelling. Our results suggest that this novel, minimally invasive mouse model can recapitulate many of the clinical features of ECC-induced systemic inflammatory response and organ injury.http://dx.doi.org/10.1155/2015/412319 |
| spellingShingle | Shuhua Luo Menglin Tang Lei Du Lina Gong Jin Xu Youwen Chen Yabo Wang Ke Lin Qi An A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation Mediators of Inflammation |
| title | A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation |
| title_full | A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation |
| title_fullStr | A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation |
| title_full_unstemmed | A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation |
| title_short | A Novel Minimal Invasive Mouse Model of Extracorporeal Circulation |
| title_sort | novel minimal invasive mouse model of extracorporeal circulation |
| url | http://dx.doi.org/10.1155/2015/412319 |
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