Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation
IntroductionPhyllosphere-associated microbes directly influence plant-pathogen interactions, and the external environment and the plant shape the phyllosphere microbiome.MethodsIn this study, we integrated 16S rRNA and ITS high-throughput sequencing to systematically investigate changes in the phyll...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1551476/full |
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| author | Shiwei Wang Yu Tan Qing Luo Xinmei Fang Hanmingyue Zhu Shuying Li Yujue Zhou Tianhui Zhu Tianhui Zhu |
| author_facet | Shiwei Wang Yu Tan Qing Luo Xinmei Fang Hanmingyue Zhu Shuying Li Yujue Zhou Tianhui Zhu Tianhui Zhu |
| author_sort | Shiwei Wang |
| collection | DOAJ |
| description | IntroductionPhyllosphere-associated microbes directly influence plant-pathogen interactions, and the external environment and the plant shape the phyllosphere microbiome.MethodsIn this study, we integrated 16S rRNA and ITS high-throughput sequencing to systematically investigate changes in the phyllosphere microbiome between symptomatic and asymptomatic walnut leaves affected by spot disease, with consideration of phenological stage progression. Additionally, we explored how abiotic (AT, DT, SCTCC & LPDD) and biotic factors (Pn & Gs) impact microbial communities.ResultsOur findings revealed significant differences in the diversity of the phyllosphere microbiome between symptomatic and asymptomatic leaves at the same phenological stage. Furthermore, the structure and function of phyllosphere-associated microbiome changed as the phenological stage progressed. Fungal taxa that related to the function Plant_Pathogen and bacterial taxa that related to the KEGG pathway functions Fatty acid biosynthesis and Biotin metabolism were increased in the symptomatic group. The keystone species driving the walnut phyllosphere microbiome was Pseudomonas spp., which substantially influenced the microbiome of symptomatic vs. asymptomatic leaves. Notably, Pseudomonas spp. interacted with Xanthomonas spp. and Pantoea spp. Correlation analysis revealed that the dew point temperature constituted the primary abiotic factor of phyllosphere bacterial community composition, whereas liquid precipitation depth dimension was identified as the dominant factor shaping fungal taxa. Additionally, leaf net photosynthetic rate and stomatal conductance were closely linked to the phyllosphere microbiome.DiscussionThese results advance our understanding of community-level microbial responses to pathogen invasion and highlight the multifactorial drivers of phyllosphere microbiome assembly. Ultimately, they contribute to predicting and managing walnut leaf-related diseases. |
| format | Article |
| id | doaj-art-97fe5fbbbcfd4e35bc3d7aa58643841d |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-97fe5fbbbcfd4e35bc3d7aa58643841d2025-08-20T01:50:45ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-04-011610.3389/fmicb.2025.15514761551476Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbationShiwei Wang0Yu Tan1Qing Luo2Xinmei Fang3Hanmingyue Zhu4Shuying Li5Yujue Zhou6Tianhui Zhu7Tianhui Zhu8College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan Province, ChinaChengdu Botanical Garden, Chengdu, Sichuan Province, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, Sichuan Province, ChinaLife Science College, Neijiang Normal University, Neijiang, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, Sichuan Province, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, Sichuan Province, ChinaCollege of Landscape Architecture, Sichuan Agricultural University, Chengdu, Sichuan Province, ChinaCollege of Forestry, Sichuan Agricultural University, Chengdu, Sichuan Province, ChinaNational Forestry and Grassland Administration Key Laboratory of Forest Resources Conservation and Ecological Safety on the Upper Reaches of the Yangtze River, Chengdu, Sichuan Province, ChinaIntroductionPhyllosphere-associated microbes directly influence plant-pathogen interactions, and the external environment and the plant shape the phyllosphere microbiome.MethodsIn this study, we integrated 16S rRNA and ITS high-throughput sequencing to systematically investigate changes in the phyllosphere microbiome between symptomatic and asymptomatic walnut leaves affected by spot disease, with consideration of phenological stage progression. Additionally, we explored how abiotic (AT, DT, SCTCC & LPDD) and biotic factors (Pn & Gs) impact microbial communities.ResultsOur findings revealed significant differences in the diversity of the phyllosphere microbiome between symptomatic and asymptomatic leaves at the same phenological stage. Furthermore, the structure and function of phyllosphere-associated microbiome changed as the phenological stage progressed. Fungal taxa that related to the function Plant_Pathogen and bacterial taxa that related to the KEGG pathway functions Fatty acid biosynthesis and Biotin metabolism were increased in the symptomatic group. The keystone species driving the walnut phyllosphere microbiome was Pseudomonas spp., which substantially influenced the microbiome of symptomatic vs. asymptomatic leaves. Notably, Pseudomonas spp. interacted with Xanthomonas spp. and Pantoea spp. Correlation analysis revealed that the dew point temperature constituted the primary abiotic factor of phyllosphere bacterial community composition, whereas liquid precipitation depth dimension was identified as the dominant factor shaping fungal taxa. Additionally, leaf net photosynthetic rate and stomatal conductance were closely linked to the phyllosphere microbiome.DiscussionThese results advance our understanding of community-level microbial responses to pathogen invasion and highlight the multifactorial drivers of phyllosphere microbiome assembly. Ultimately, they contribute to predicting and managing walnut leaf-related diseases.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1551476/fullplant-microbe interactionsphyllosphere microbiotaleaf diseaseJuglans regia L.high-throughput sequencing |
| spellingShingle | Shiwei Wang Yu Tan Qing Luo Xinmei Fang Hanmingyue Zhu Shuying Li Yujue Zhou Tianhui Zhu Tianhui Zhu Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation Frontiers in Microbiology plant-microbe interactions phyllosphere microbiota leaf disease Juglans regia L. high-throughput sequencing |
| title | Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation |
| title_full | Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation |
| title_fullStr | Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation |
| title_full_unstemmed | Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation |
| title_short | Temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation |
| title_sort | temporal dynamics of walnut phyllosphere microbiota under synergistic pathogen exposure and environmental perturbation |
| topic | plant-microbe interactions phyllosphere microbiota leaf disease Juglans regia L. high-throughput sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1551476/full |
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