Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia
Abstract Muscles are crucial for balance and walking, activities which depend specifically on the lower extremity muscles. Therefore, the evaluation of stroke induced atrophy and paralysis is essential; however, determining the extent of damage in the days after its occurrence remains challenging. I...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-78324-3 |
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| author | Subok Kim Sanghun Jang Onseok Lee |
| author_facet | Subok Kim Sanghun Jang Onseok Lee |
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| description | Abstract Muscles are crucial for balance and walking, activities which depend specifically on the lower extremity muscles. Therefore, the evaluation of stroke induced atrophy and paralysis is essential; however, determining the extent of damage in the days after its occurrence remains challenging. In this study, we evaluated ischemic stroke-induced soleus muscle damage in gerbils using synchrotron radiation X-ray micro-computed tomography (SR-µCT), comparing a control group (n = 3), animals 7 days after stroke (7 d, n = 3), and animals 14 days after stroke (14 d, n = 3). The left muscle was paralyzed, whereas the right muscle was not. Subsequently, we quantified the assessment by segmenting the soleus muscle based on the extracellular space/matrix and fiber region to determine the degree of damage. The muscle fiber-to-extracellular space/matrix ratio were significantly damaged due to paralysis on the left side (control vs. 14 d, P = 0.040). Muscle area was significantly different at 14 d between the left and right sides (P = 0.010). Additionally, the left local fascicle surface area, thickness, global pennation angle, and local fascicle angle were significantly different between the control and 14 d groups (P = 0.002, P = 0.007, P = 0.005, and P = 0.014 respectively). These findings underscore the potential of post-stroke animal studies in improving rehabilitation treatment for the central nervous system by assessing the degree of muscle recovery. |
| format | Article |
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| spelling | doaj-art-97eb65cc01e44b76aa8d58a651436dff2025-08-20T02:13:55ZengNature PortfolioScientific Reports2045-23222024-11-0114111410.1038/s41598-024-78324-3Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemiaSubok Kim0Sanghun Jang1Onseok Lee2Department of Software Convergence, Graduate School, Soonchunhyang UniversityDepartment of Physical Therapy, College of Health and Life Sciences, Korea National University of TransportationDepartment of Software Convergence, Graduate School, Soonchunhyang UniversityAbstract Muscles are crucial for balance and walking, activities which depend specifically on the lower extremity muscles. Therefore, the evaluation of stroke induced atrophy and paralysis is essential; however, determining the extent of damage in the days after its occurrence remains challenging. In this study, we evaluated ischemic stroke-induced soleus muscle damage in gerbils using synchrotron radiation X-ray micro-computed tomography (SR-µCT), comparing a control group (n = 3), animals 7 days after stroke (7 d, n = 3), and animals 14 days after stroke (14 d, n = 3). The left muscle was paralyzed, whereas the right muscle was not. Subsequently, we quantified the assessment by segmenting the soleus muscle based on the extracellular space/matrix and fiber region to determine the degree of damage. The muscle fiber-to-extracellular space/matrix ratio were significantly damaged due to paralysis on the left side (control vs. 14 d, P = 0.040). Muscle area was significantly different at 14 d between the left and right sides (P = 0.010). Additionally, the left local fascicle surface area, thickness, global pennation angle, and local fascicle angle were significantly different between the control and 14 d groups (P = 0.002, P = 0.007, P = 0.005, and P = 0.014 respectively). These findings underscore the potential of post-stroke animal studies in improving rehabilitation treatment for the central nervous system by assessing the degree of muscle recovery.https://doi.org/10.1038/s41598-024-78324-3Ischemic strokeSynchrotron radiationMuscleDamageFiber |
| spellingShingle | Subok Kim Sanghun Jang Onseok Lee Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia Scientific Reports Ischemic stroke Synchrotron radiation Muscle Damage Fiber |
| title | Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia |
| title_full | Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia |
| title_fullStr | Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia |
| title_full_unstemmed | Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia |
| title_short | Muscle structure assessment using synchrotron radiation X-ray micro-computed tomography in murine with cerebral ischemia |
| title_sort | muscle structure assessment using synchrotron radiation x ray micro computed tomography in murine with cerebral ischemia |
| topic | Ischemic stroke Synchrotron radiation Muscle Damage Fiber |
| url | https://doi.org/10.1038/s41598-024-78324-3 |
| work_keys_str_mv | AT subokkim musclestructureassessmentusingsynchrotronradiationxraymicrocomputedtomographyinmurinewithcerebralischemia AT sanghunjang musclestructureassessmentusingsynchrotronradiationxraymicrocomputedtomographyinmurinewithcerebralischemia AT onseoklee musclestructureassessmentusingsynchrotronradiationxraymicrocomputedtomographyinmurinewithcerebralischemia |