Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation
A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of lamivudine in pure and tablet formulations. A Thermo BDS C18 column in isocratic mode, with a mobile phase consisting of 0.01 M ammonium dihydrogen orthophosphate buffer adjusted to pH 2.48 by using formi...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2010/490328 |
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| author | S. K. Patro S. R. Swain V. J. Patro N. S. K. Choudhury |
| author_facet | S. K. Patro S. R. Swain V. J. Patro N. S. K. Choudhury |
| author_sort | S. K. Patro |
| collection | DOAJ |
| description | A new, simple, specific, accurate and precise RP-HPLC method was developed for determination of lamivudine in pure and tablet formulations. A Thermo BDS C18 column in isocratic mode, with a mobile phase consisting of 0.01 M ammonium dihydrogen orthophosphate buffer adjusted to pH 2.48 by using formic acid and methanol in the ratio of 50:50 was used. The flow rate was set at 0.6 mL/min and UV detection was carried out at 264 nm. The retention time of lamivudine and nevirapine were 2.825 min and 4.958 min respectively. The method was validated for linearity, precision, robustness and recovery. Linearity for lamivudine was found in the range of 50-175 μg/mL. Hence, it can be applied for routine quality control of lamivudine in bulk and pharmaceutical formulations. |
| format | Article |
| id | doaj-art-97dd074e6afd45b88c54f8e64f104c93 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-97dd074e6afd45b88c54f8e64f104c932025-02-03T05:50:28ZengWileyE-Journal of Chemistry0973-49452090-98102010-01-017111712210.1155/2010/490328Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical PreparationS. K. Patro0S. R. Swain1V. J. Patro2N. S. K. Choudhury3Department of Pharmaceutical Analysis & Quality Assurance, Institute of Pharmacy and Technology, Salipur, Cuttack, Orissa-754202, IndiaDepartment of Pharmaceutical Analysis & Quality Assurance, Institute of Pharmacy and Technology, Salipur, Cuttack, Orissa-754202, IndiaCollege of Pharmaceutical Sciences, Mohuda, Berhampur, Ganjam, Orissa, IndiaDepartment of Pharmacy, S.C.B. Medical College, Cuttack, Orissa, IndiaA new, simple, specific, accurate and precise RP-HPLC method was developed for determination of lamivudine in pure and tablet formulations. A Thermo BDS C18 column in isocratic mode, with a mobile phase consisting of 0.01 M ammonium dihydrogen orthophosphate buffer adjusted to pH 2.48 by using formic acid and methanol in the ratio of 50:50 was used. The flow rate was set at 0.6 mL/min and UV detection was carried out at 264 nm. The retention time of lamivudine and nevirapine were 2.825 min and 4.958 min respectively. The method was validated for linearity, precision, robustness and recovery. Linearity for lamivudine was found in the range of 50-175 μg/mL. Hence, it can be applied for routine quality control of lamivudine in bulk and pharmaceutical formulations.http://dx.doi.org/10.1155/2010/490328 |
| spellingShingle | S. K. Patro S. R. Swain V. J. Patro N. S. K. Choudhury Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation E-Journal of Chemistry |
| title | Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation |
| title_full | Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation |
| title_fullStr | Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation |
| title_full_unstemmed | Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation |
| title_short | Development and Validation of High Performance Liquid Chromatographic Method for Determination of Lamivudine from Pharmaceutical Preparation |
| title_sort | development and validation of high performance liquid chromatographic method for determination of lamivudine from pharmaceutical preparation |
| url | http://dx.doi.org/10.1155/2010/490328 |
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