Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken
ABSTRACT Background Stress associated with changes in host immunity occurs in response to altered environmental conditions, endogenous imbalances, infectious agents and harmful stimuli. The importance of genetic diversity in chickens has increased due to individual immune differences towards resista...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70062 |
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| author | Afra Vatankhah Gholamreza Nikbakht Brujeni Atefeh Esmailnejad |
| author_facet | Afra Vatankhah Gholamreza Nikbakht Brujeni Atefeh Esmailnejad |
| author_sort | Afra Vatankhah |
| collection | DOAJ |
| description | ABSTRACT Background Stress associated with changes in host immunity occurs in response to altered environmental conditions, endogenous imbalances, infectious agents and harmful stimuli. The importance of genetic diversity in chickens has increased due to individual immune differences towards resistance and susceptibility to various stimuli. Objectives This study aimed to investigate the association of major histocompatibility complex (MHC) polymorphism with acute phase response (APR) in Ross 308 broiler chickens. Methods The allelic diversity of the LEI0258 microsatellite marker was determined in 120 Ross broilers. In addition, acute phase proteins (APPs), including serum amyloid A (SAA) and alpha‐1‐acid glycoprotein (AGP), were analysed as markers of the APR. Furthermore, leukocyte count and the heterophil/lymphocyte ratio (H/L ratio) were examined. The antibody response to the Newcastle disease vaccine (NDV) was also measured to assess humoral mediated immunity. Lastly, the correlation between immune responses and MHC alleles was investigated to identify the most effective alleles in a stress‐related situation. Results A total of six alleles, ranging from 195 to 448 bp, were identified. Association study revealed a significant influence of MHC alleles on APPs in Ross population (p < 0.05). Notably, Allele 448 had a significant correlation with SAA concentration and the H/L ratio. Allele 207 displayed a positive association with AGP concentration, whereas Allele 195 showed a negative association. Furthermore, a significant association was observed between Allele 448 and basopenia, as well as between Allele 195 and monocytosis. Conclusions Results confirmed the significance of MHC as a candidate gene marker for immune responses, which supports its use for vaccine design, genetic improvement of disease‐resistant traits and resource conservation in commercial broiler chickens. |
| format | Article |
| id | doaj-art-97c1b22e3e304353961b94787c6b92be |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-97c1b22e3e304353961b94787c6b92be2025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70062Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler ChickenAfra Vatankhah0Gholamreza Nikbakht Brujeni1Atefeh Esmailnejad2Department of Microbiology and Immunology Faculty of Veterinary Medicine University of Tehran Tehran IranDepartment of Microbiology and Immunology Faculty of Veterinary Medicine University of Tehran Tehran IranDepartment of Pathobiology Faculty of Veterinary Medicine University of Shiraz Shiraz IranABSTRACT Background Stress associated with changes in host immunity occurs in response to altered environmental conditions, endogenous imbalances, infectious agents and harmful stimuli. The importance of genetic diversity in chickens has increased due to individual immune differences towards resistance and susceptibility to various stimuli. Objectives This study aimed to investigate the association of major histocompatibility complex (MHC) polymorphism with acute phase response (APR) in Ross 308 broiler chickens. Methods The allelic diversity of the LEI0258 microsatellite marker was determined in 120 Ross broilers. In addition, acute phase proteins (APPs), including serum amyloid A (SAA) and alpha‐1‐acid glycoprotein (AGP), were analysed as markers of the APR. Furthermore, leukocyte count and the heterophil/lymphocyte ratio (H/L ratio) were examined. The antibody response to the Newcastle disease vaccine (NDV) was also measured to assess humoral mediated immunity. Lastly, the correlation between immune responses and MHC alleles was investigated to identify the most effective alleles in a stress‐related situation. Results A total of six alleles, ranging from 195 to 448 bp, were identified. Association study revealed a significant influence of MHC alleles on APPs in Ross population (p < 0.05). Notably, Allele 448 had a significant correlation with SAA concentration and the H/L ratio. Allele 207 displayed a positive association with AGP concentration, whereas Allele 195 showed a negative association. Furthermore, a significant association was observed between Allele 448 and basopenia, as well as between Allele 195 and monocytosis. Conclusions Results confirmed the significance of MHC as a candidate gene marker for immune responses, which supports its use for vaccine design, genetic improvement of disease‐resistant traits and resource conservation in commercial broiler chickens.https://doi.org/10.1002/vms3.70062acute phase proteins | chicken | inflammation | LEI0258 | major histocompatibility complex (MHC) |
| spellingShingle | Afra Vatankhah Gholamreza Nikbakht Brujeni Atefeh Esmailnejad Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken Veterinary Medicine and Science acute phase proteins | chicken | inflammation | LEI0258 | major histocompatibility complex (MHC) |
| title | Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken |
| title_full | Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken |
| title_fullStr | Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken |
| title_full_unstemmed | Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken |
| title_short | Association of Major Histocompatibility Complex Polymorphism With Acute Phase Response in Broiler Chicken |
| title_sort | association of major histocompatibility complex polymorphism with acute phase response in broiler chicken |
| topic | acute phase proteins | chicken | inflammation | LEI0258 | major histocompatibility complex (MHC) |
| url | https://doi.org/10.1002/vms3.70062 |
| work_keys_str_mv | AT afravatankhah associationofmajorhistocompatibilitycomplexpolymorphismwithacutephaseresponseinbroilerchicken AT gholamrezanikbakhtbrujeni associationofmajorhistocompatibilitycomplexpolymorphismwithacutephaseresponseinbroilerchicken AT atefehesmailnejad associationofmajorhistocompatibilitycomplexpolymorphismwithacutephaseresponseinbroilerchicken |