Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period
Introduction: The biological maturity period is the time or tempo of progress toward adulthood or maturity. The hormonal fluctuations play an important role in changing the characteristics of human body tissues during the biological maturity period. However, changes in tissue characteristics during...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
FEADEF
2025-01-01
|
| Series: | Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación |
| Subjects: | |
| Online Access: | https://recyt.fecyt.es/index.php/retos/article/view/110950 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587234381922304 |
|---|---|
| author | Sinta Ayu Choiriyah Bambang Purwanto Viskasari Pintoko Kalanjati Raden Argarini Anisah Rosydiyah |
| author_facet | Sinta Ayu Choiriyah Bambang Purwanto Viskasari Pintoko Kalanjati Raden Argarini Anisah Rosydiyah |
| author_sort | Sinta Ayu Choiriyah |
| collection | DOAJ |
| description |
Introduction: The biological maturity period is the time or tempo of progress toward adulthood or maturity. The hormonal fluctuations play an important role in changing the characteristics of human body tissues during the biological maturity period. However, changes in tissue characteristics during biological maturity period have not been revealed. Skeletal muscle tissue is one of those believed to experience changes. Muscle fibers are thought to experience switching types from type 2 muscle fibers to type 1 muscle fibers. The transition of muscle fibers from type 2 to type 1 requires miR-499 activity from the expression of the Myh7b gene. MiR-499 directly inhibits FNIP-1. However, the biological maturity period of changing FNIP-1 expression has not been confirmed.
Objective: This study aimed to analyse the effect of the biological maturity period on FNIP-1 expression and capillary density in the gastrocnemius muscle.
Methodology: Thirty-six male mice were divided into mature groups aged eight weeks and immature groups aged four weeks. This study analyzed FNIP-1 and capillary density gastrocnemius muscle of mice using immunohistochemistry.
Results: FNIP-1 expression test showed higher in immature mice than mature mice. In comparison, the capillary density test and endurance showed higher expression in mature mice than in immature mice.
Conclusion: This study concludes maturation was characterized by a low distribution of FNIP-1 expression in the gastrocnemius muscle and a longer duration ability to run on the treadmill. Unfortunately the capillary density was not a specific mark to determine maturation in mice.
|
| format | Article |
| id | doaj-art-97b4a7de8d824cdebdc2a1510b2b580e |
| institution | Kabale University |
| issn | 1579-1726 1988-2041 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | FEADEF |
| record_format | Article |
| series | Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación |
| spelling | doaj-art-97b4a7de8d824cdebdc2a1510b2b580e2025-01-24T17:12:16ZengFEADEFRetos: Nuevas Tendencias en Educación Física, Deportes y Recreación1579-17261988-20412025-01-016410.47197/retos.v64.110950Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation periodSinta Ayu Choiriyah0Bambang Purwanto1Viskasari Pintoko Kalanjati2Raden Argarini3Anisah RosydiyahSport Health Science Master Program, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Physiology, Faculty of Medicine, Universitas Airlangga, Surabaya, IndonesiaDepartment of Anatomy, Histology, and Pharmacology, Faculty of Medicine, Airlangga University Surabaya, IndonesiaDepartment of Physiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia Introduction: The biological maturity period is the time or tempo of progress toward adulthood or maturity. The hormonal fluctuations play an important role in changing the characteristics of human body tissues during the biological maturity period. However, changes in tissue characteristics during biological maturity period have not been revealed. Skeletal muscle tissue is one of those believed to experience changes. Muscle fibers are thought to experience switching types from type 2 muscle fibers to type 1 muscle fibers. The transition of muscle fibers from type 2 to type 1 requires miR-499 activity from the expression of the Myh7b gene. MiR-499 directly inhibits FNIP-1. However, the biological maturity period of changing FNIP-1 expression has not been confirmed. Objective: This study aimed to analyse the effect of the biological maturity period on FNIP-1 expression and capillary density in the gastrocnemius muscle. Methodology: Thirty-six male mice were divided into mature groups aged eight weeks and immature groups aged four weeks. This study analyzed FNIP-1 and capillary density gastrocnemius muscle of mice using immunohistochemistry. Results: FNIP-1 expression test showed higher in immature mice than mature mice. In comparison, the capillary density test and endurance showed higher expression in mature mice than in immature mice. Conclusion: This study concludes maturation was characterized by a low distribution of FNIP-1 expression in the gastrocnemius muscle and a longer duration ability to run on the treadmill. Unfortunately the capillary density was not a specific mark to determine maturation in mice. https://recyt.fecyt.es/index.php/retos/article/view/110950FNIP-1MaturityEnduranceCapillaryMuscleHealthy Lifestyle |
| spellingShingle | Sinta Ayu Choiriyah Bambang Purwanto Viskasari Pintoko Kalanjati Raden Argarini Anisah Rosydiyah Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period Retos: Nuevas Tendencias en Educación Física, Deportes y Recreación FNIP-1 Maturity Endurance Capillary Muscle Healthy Lifestyle |
| title | Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period |
| title_full | Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period |
| title_fullStr | Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period |
| title_full_unstemmed | Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period |
| title_short | Folliculin Interacting Protein 1 (FNIP-1) expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period |
| title_sort | folliculin interacting protein 1 fnip 1 expression and capillary density in gastrocnemius muscle tissue of mice after biological maturation period |
| topic | FNIP-1 Maturity Endurance Capillary Muscle Healthy Lifestyle |
| url | https://recyt.fecyt.es/index.php/retos/article/view/110950 |
| work_keys_str_mv | AT sintaayuchoiriyah folliculininteractingprotein1fnip1expressionandcapillarydensityingastrocnemiusmuscletissueofmiceafterbiologicalmaturationperiod AT bambangpurwanto folliculininteractingprotein1fnip1expressionandcapillarydensityingastrocnemiusmuscletissueofmiceafterbiologicalmaturationperiod AT viskasaripintokokalanjati folliculininteractingprotein1fnip1expressionandcapillarydensityingastrocnemiusmuscletissueofmiceafterbiologicalmaturationperiod AT radenargarini folliculininteractingprotein1fnip1expressionandcapillarydensityingastrocnemiusmuscletissueofmiceafterbiologicalmaturationperiod AT anisahrosydiyah folliculininteractingprotein1fnip1expressionandcapillarydensityingastrocnemiusmuscletissueofmiceafterbiologicalmaturationperiod |