The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue
The aim of this study was to investigate the possible protective effects of infliximab on expression of laminin, anti-TNF, and NFκB in the rat hepatic cells after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: Control (C), sham I/R (ISC), and I/R+ i...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2016/1738430 |
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| author | Remzi Adnan Akdogan Yildiray Kalkan Levent Tümkaya Halil Rakici Elif Akdogan |
| author_facet | Remzi Adnan Akdogan Yildiray Kalkan Levent Tümkaya Halil Rakici Elif Akdogan |
| author_sort | Remzi Adnan Akdogan |
| collection | DOAJ |
| description | The aim of this study was to investigate the possible protective effects of infliximab on expression of laminin, anti-TNF, and NFκB in the rat hepatic cells after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: Control (C), sham I/R (ISC), and I/R+ infliximab (ISC inf); each group comprised 10 animals. C group animals underwent laparotomy without I/R injury. In ISC groups after undergoing laparotomy, 1 hour of superior mesenteric artery ligation was done, which was followed by 1 hour of reperfusion. In the ISC inf group, 3 days before I/R, infliximab (3 mg/kg) was administered intravenously. All animals were killed at the end of reperfusion and hepatic tissue samples were obtained for histopathological and histochemical investigations in all groups. Laminin, anti-TNF, and NFκB immunoreactivity were performed for all groups. ISC caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Infliximab treatment significantly attenuated the severity of intestinal I/R injury and it is shown by laminin, anti-TNF, and NFκB immunoreactivity. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects on hepatic cells in the experimental intestinal I/R model of rats. |
| format | Article |
| id | doaj-art-978eb025fa38442a8294278de6dce105 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-978eb025fa38442a8294278de6dce1052025-02-03T01:02:17ZengWileyGastroenterology Research and Practice1687-61211687-630X2016-01-01201610.1155/2016/17384301738430The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver TissueRemzi Adnan Akdogan0Yildiray Kalkan1Levent Tümkaya2Halil Rakici3Elif Akdogan4School of Medicine, Department of Gastroenterology, Recep Tayyip Erdoğan University, 53100 Rize, TurkeySchool of Medicine, Department of Embriology and Histology, Recep Tayyip Erdoğan University, 53100 Rize, TurkeySchool of Medicine, Department of Embriology and Histology, Recep Tayyip Erdoğan University, 53100 Rize, TurkeySchool of Medicine, Department of Gastroenterology, Recep Tayyip Erdoğan University, 53100 Rize, TurkeySchool of Medicine, Department of Hematology, Recep Tayyip Erdoğan University, 53100 Rize, TurkeyThe aim of this study was to investigate the possible protective effects of infliximab on expression of laminin, anti-TNF, and NFκB in the rat hepatic cells after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: Control (C), sham I/R (ISC), and I/R+ infliximab (ISC inf); each group comprised 10 animals. C group animals underwent laparotomy without I/R injury. In ISC groups after undergoing laparotomy, 1 hour of superior mesenteric artery ligation was done, which was followed by 1 hour of reperfusion. In the ISC inf group, 3 days before I/R, infliximab (3 mg/kg) was administered intravenously. All animals were killed at the end of reperfusion and hepatic tissue samples were obtained for histopathological and histochemical investigations in all groups. Laminin, anti-TNF, and NFκB immunoreactivity were performed for all groups. ISC caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Infliximab treatment significantly attenuated the severity of intestinal I/R injury and it is shown by laminin, anti-TNF, and NFκB immunoreactivity. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects on hepatic cells in the experimental intestinal I/R model of rats.http://dx.doi.org/10.1155/2016/1738430 |
| spellingShingle | Remzi Adnan Akdogan Yildiray Kalkan Levent Tümkaya Halil Rakici Elif Akdogan The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue Gastroenterology Research and Practice |
| title | The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue |
| title_full | The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue |
| title_fullStr | The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue |
| title_full_unstemmed | The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue |
| title_short | The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue |
| title_sort | effects of infliximab on laminin nfκb and anti tnf expression through its effect on ischemic liver tissue |
| url | http://dx.doi.org/10.1155/2016/1738430 |
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