Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection
For this article, we evaluated whether wounds would affect the pathogenicity and virulence of <i>Colletotrichum</i> sp. isolates on <i>Musa</i> spp. banana cultivars. We further assessed the potential of cross-colonization with other fruit species and investigated the molecul...
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2024-12-01
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| author | Maysa C. Santos Luis O. Viteri Sabrina H. Araujo Dalmarcia C. Mourão Marcos P. Câmara Ana G. Amaral Eugênio E. Oliveira Gil Rodrigues dos Santos |
| author_facet | Maysa C. Santos Luis O. Viteri Sabrina H. Araujo Dalmarcia C. Mourão Marcos P. Câmara Ana G. Amaral Eugênio E. Oliveira Gil Rodrigues dos Santos |
| author_sort | Maysa C. Santos |
| collection | DOAJ |
| description | For this article, we evaluated whether wounds would affect the pathogenicity and virulence of <i>Colletotrichum</i> sp. isolates on <i>Musa</i> spp. banana cultivars. We further assessed the potential of cross-colonization with other fruit species and investigated the molecular and phylogenetic characterization of the most virulent isolates. Firstly, we collected dwarf bananas showing anthracnose symptoms from commercial markets in the city of Gurupi, Tocantins State, Brazil, and isolated <i>Colletotrichum</i> sp. under controlled conditions prior to identification. The virulence was assessed on wounded and unwounded banana fruits, identifying the most virulent isolate by exposure tests on fruits of the “prata”, “maçã”, “marmelo”, and “terra” banana cultivars. We also subjected specimens of mango (<i>Mangifera indica</i>), papaya (<i>Carica papaya</i>), and apple (<i>Malus domestica</i>) fruits to the exposure tests. Our results indicated that pathogenicity varies with the isolate (with C2, C8, and C10 as the most virulent), fruit condition (wounded fruits are the most susceptible), and cultivars (terra, marmela, and maça are the most susceptible). All isolates were more virulent on wounded bananas, while those on unwounded ones showed lower virulence. Among the banana cultivars, “prata” fruits were the most susceptible, regardless of wounding. Additionally, <i>Colletotrichum</i> isolates from dwarf bananas were pathogenic to mango, papaya, and apple fruits. Furthermore, our results demonstrated that the most virulent isolates belong to the species <i>C. musae</i>. Collectively, our findings reinforce the relevance of minimizing post-harvest wounds on banana fruits and highlight the risks of cross-infection when storing bananas alongside other fruit species. |
| format | Article |
| id | doaj-art-9783e89c78f54e42941b3652e0d868f6 |
| institution | Kabale University |
| issn | 2036-7481 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microbiology Research |
| spelling | doaj-art-9783e89c78f54e42941b3652e0d868f62025-01-24T13:41:42ZengMDPI AGMicrobiology Research2036-74812024-12-01161410.3390/microbiolres16010004Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-InfectionMaysa C. Santos0Luis O. Viteri1Sabrina H. Araujo2Dalmarcia C. Mourão3Marcos P. Câmara4Ana G. Amaral5Eugênio E. Oliveira6Gil Rodrigues dos Santos7Programa de Pós-Graduação em Produção Vegetal, Universidade Federal do Tocantins, Gurupi 77402-970, BrazilPrograma de Pós-Graduação em Ciências Florestais e Ambientais, Universidade Federal do Tocantins, Gurupi 77402-970, BrazilPrograma de Pós-Graduação em Produção Vegetal, Universidade Federal do Tocantins, Gurupi 77402-970, BrazilPrograma de Pós-Graduação em Produção Vegetal, Universidade Federal do Tocantins, Gurupi 77402-970, BrazilDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife 52171-900, BrazilDepartamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife 52171-900, BrazilPrograma de Pós-Graduação em Biotecnologia, Universidade Federal do Tocantins, Gurupi 77402-970, BrazilPrograma de Pós-Graduação em Produção Vegetal, Universidade Federal do Tocantins, Gurupi 77402-970, BrazilFor this article, we evaluated whether wounds would affect the pathogenicity and virulence of <i>Colletotrichum</i> sp. isolates on <i>Musa</i> spp. banana cultivars. We further assessed the potential of cross-colonization with other fruit species and investigated the molecular and phylogenetic characterization of the most virulent isolates. Firstly, we collected dwarf bananas showing anthracnose symptoms from commercial markets in the city of Gurupi, Tocantins State, Brazil, and isolated <i>Colletotrichum</i> sp. under controlled conditions prior to identification. The virulence was assessed on wounded and unwounded banana fruits, identifying the most virulent isolate by exposure tests on fruits of the “prata”, “maçã”, “marmelo”, and “terra” banana cultivars. We also subjected specimens of mango (<i>Mangifera indica</i>), papaya (<i>Carica papaya</i>), and apple (<i>Malus domestica</i>) fruits to the exposure tests. Our results indicated that pathogenicity varies with the isolate (with C2, C8, and C10 as the most virulent), fruit condition (wounded fruits are the most susceptible), and cultivars (terra, marmela, and maça are the most susceptible). All isolates were more virulent on wounded bananas, while those on unwounded ones showed lower virulence. Among the banana cultivars, “prata” fruits were the most susceptible, regardless of wounding. Additionally, <i>Colletotrichum</i> isolates from dwarf bananas were pathogenic to mango, papaya, and apple fruits. Furthermore, our results demonstrated that the most virulent isolates belong to the species <i>C. musae</i>. Collectively, our findings reinforce the relevance of minimizing post-harvest wounds on banana fruits and highlight the risks of cross-infection when storing bananas alongside other fruit species.https://www.mdpi.com/2036-7481/16/1/4anthracnosetropical fruitspost-harvest diseasescross-infection |
| spellingShingle | Maysa C. Santos Luis O. Viteri Sabrina H. Araujo Dalmarcia C. Mourão Marcos P. Câmara Ana G. Amaral Eugênio E. Oliveira Gil Rodrigues dos Santos Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection Microbiology Research anthracnose tropical fruits post-harvest diseases cross-infection |
| title | Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection |
| title_full | Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection |
| title_fullStr | Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection |
| title_full_unstemmed | Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection |
| title_short | Molecular Characterization and Pathogenicity of <i>Colletotrichum</i> on Banana Fruits: Wound Effects on Virulence and Cross-Infection |
| title_sort | molecular characterization and pathogenicity of i colletotrichum i on banana fruits wound effects on virulence and cross infection |
| topic | anthracnose tropical fruits post-harvest diseases cross-infection |
| url | https://www.mdpi.com/2036-7481/16/1/4 |
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