Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus
The present study was conducted to clarify the effects of Lactobacillus salivarius (LS) ATCC 11741 and pectin (PE) on growth performance, digestive enzymes activity, gut microbiota composition, immune parameters, antioxidant defense as well as disease resistance against Aeromonas hydrophila in narro...
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Wiley
2022-01-01
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| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2022/1861761 |
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| author | Samyah Darwish Saddig Jastaniah Hafsan Hafsan Cheng-jui Tseng Yasir Salam Karim Mohammed Ubaid Hamza Noora M. Hameed Sura Hasan Al-Zubaidi Saif Sabbar Kemil Almotlaq Ghulam Yasin A. Heri Iswanto Mahnaz Dadras Mohammad Mansouri Chorehi |
| author_facet | Samyah Darwish Saddig Jastaniah Hafsan Hafsan Cheng-jui Tseng Yasir Salam Karim Mohammed Ubaid Hamza Noora M. Hameed Sura Hasan Al-Zubaidi Saif Sabbar Kemil Almotlaq Ghulam Yasin A. Heri Iswanto Mahnaz Dadras Mohammad Mansouri Chorehi |
| author_sort | Samyah Darwish Saddig Jastaniah |
| collection | DOAJ |
| description | The present study was conducted to clarify the effects of Lactobacillus salivarius (LS) ATCC 11741 and pectin (PE) on growth performance, digestive enzymes activity, gut microbiota composition, immune parameters, antioxidant defense as well as disease resistance against Aeromonas hydrophila in narrow-clawed crayfish, Postantacus leptodactylus. During 18 weeks trial feeding, 525 narrow-clawed crayfish juvenile (8.07±0.1 g) fed with seven experimental diets including control (basal diet), LS1 (1×107 CFU/g), LS2 (1×109 CFU/g), PE1 (5 g/kg), PE2 (10 g/kg), LS1PE1 (1×107 CFU/g +5 g/kg), and LS2PE2 (1×109 CFU/g +10 g/kg). After 18 weeks, growth parameters (final weight, weight gain, and specific growth rate) and feed conversion rate were significantly improved in all treatments (P<0.05). Besides, diets incorporated with LS1PE1 and LS2PE2 significantly increased the activity of amylase and protease enzymes compared to LS1, LS2, and control groups (P<0.05). Microbiological analyses revealed that the total heterotrophic bacteria count (TVC) and lactic acid bacteria (LAB) of narrow-clawed crayfish fed diets containing LS1, LS2, LS1PE1, and LS2PE2 were higher than control group. The highest total haemocyte count (THC), large-granular (LGC) and semigranular cells (SGC) count, and hyaline count (HC) was obtained in LS1PE1 (P<0.05). Similarly, higher immunity activity (lysozyme (LYZ), phenoloxidase (PO), nitroxidesynthetase (NOs), and alkaline phosphatase (AKP)) observed in the LS1PE1 treatment compared to the control group (P<0.05). The glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity remarkably enhanced in LS1PE1 and LS2PE2, while malondialdehyde (MDA) content reduced in these two treatments. In addition, specimens belonging to LS1, LS2, PE2, LS1PE1, and LS2PE2 groups presented higher resistance against A. hydrophila compared to the control group. In conclusion, feeding narrow-clawed crayfish with synbiotic had higher efficiency on growth parameters, immunocompetence, and disease resistance compared to single consumption of prebiotics and probiotics. |
| format | Article |
| id | doaj-art-977acf60dcf04c18aee890ef9529f04b |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
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| series | Aquaculture Nutrition |
| spelling | doaj-art-977acf60dcf04c18aee890ef9529f04b2025-02-03T06:08:44ZengWileyAquaculture Nutrition1365-20952022-01-01202210.1155/2022/1861761Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylusSamyah Darwish Saddig Jastaniah0Hafsan Hafsan1Cheng-jui Tseng2Yasir Salam Karim3Mohammed Ubaid Hamza4Noora M. Hameed5Sura Hasan Al-Zubaidi6Saif Sabbar Kemil Almotlaq7Ghulam Yasin8A. Heri Iswanto9Mahnaz Dadras10Mohammad Mansouri Chorehi11Biological Sciences DepartmentBiology DepartmentAssistant ProfessorDepartment of PharmacyMedical Technical CollegeAnesthesia TechniquesAnesthesia Techniques DepartmentMicrobiology Research GroupDepartment of BotanyPublic Health DepartmentDepartment of FisheriesDepartment of FisheriesThe present study was conducted to clarify the effects of Lactobacillus salivarius (LS) ATCC 11741 and pectin (PE) on growth performance, digestive enzymes activity, gut microbiota composition, immune parameters, antioxidant defense as well as disease resistance against Aeromonas hydrophila in narrow-clawed crayfish, Postantacus leptodactylus. During 18 weeks trial feeding, 525 narrow-clawed crayfish juvenile (8.07±0.1 g) fed with seven experimental diets including control (basal diet), LS1 (1×107 CFU/g), LS2 (1×109 CFU/g), PE1 (5 g/kg), PE2 (10 g/kg), LS1PE1 (1×107 CFU/g +5 g/kg), and LS2PE2 (1×109 CFU/g +10 g/kg). After 18 weeks, growth parameters (final weight, weight gain, and specific growth rate) and feed conversion rate were significantly improved in all treatments (P<0.05). Besides, diets incorporated with LS1PE1 and LS2PE2 significantly increased the activity of amylase and protease enzymes compared to LS1, LS2, and control groups (P<0.05). Microbiological analyses revealed that the total heterotrophic bacteria count (TVC) and lactic acid bacteria (LAB) of narrow-clawed crayfish fed diets containing LS1, LS2, LS1PE1, and LS2PE2 were higher than control group. The highest total haemocyte count (THC), large-granular (LGC) and semigranular cells (SGC) count, and hyaline count (HC) was obtained in LS1PE1 (P<0.05). Similarly, higher immunity activity (lysozyme (LYZ), phenoloxidase (PO), nitroxidesynthetase (NOs), and alkaline phosphatase (AKP)) observed in the LS1PE1 treatment compared to the control group (P<0.05). The glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity remarkably enhanced in LS1PE1 and LS2PE2, while malondialdehyde (MDA) content reduced in these two treatments. In addition, specimens belonging to LS1, LS2, PE2, LS1PE1, and LS2PE2 groups presented higher resistance against A. hydrophila compared to the control group. In conclusion, feeding narrow-clawed crayfish with synbiotic had higher efficiency on growth parameters, immunocompetence, and disease resistance compared to single consumption of prebiotics and probiotics.http://dx.doi.org/10.1155/2022/1861761 |
| spellingShingle | Samyah Darwish Saddig Jastaniah Hafsan Hafsan Cheng-jui Tseng Yasir Salam Karim Mohammed Ubaid Hamza Noora M. Hameed Sura Hasan Al-Zubaidi Saif Sabbar Kemil Almotlaq Ghulam Yasin A. Heri Iswanto Mahnaz Dadras Mohammad Mansouri Chorehi Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus Aquaculture Nutrition |
| title | Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus |
| title_full | Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus |
| title_fullStr | Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus |
| title_full_unstemmed | Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus |
| title_short | Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus |
| title_sort | effects of dietary pectin and lactobacillus salivarius atcc 11741 on growth performance immunocompetence gut microbiota antioxidant capacity and disease resistance in narrow clawed crayfish postantacus leptodactylus |
| url | http://dx.doi.org/10.1155/2022/1861761 |
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