Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas
Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in r...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/528098 |
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| author | Hajnalka Rajnai Ivett Teleki Gergo Kiszner Nora Meggyesházi Peter Balla Tamas Vancsik Gyorgyi Muzes Judit Csomor Andras Matolcsy Tibor Krenacs |
| author_facet | Hajnalka Rajnai Ivett Teleki Gergo Kiszner Nora Meggyesházi Peter Balla Tamas Vancsik Gyorgyi Muzes Judit Csomor Andras Matolcsy Tibor Krenacs |
| author_sort | Hajnalka Rajnai |
| collection | DOAJ |
| description | Follicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell clusters (resembling to “ex vivo” germinal centers) with Gap27 peptide, mimicking the 2nd extracellular loop of Cx43 protein, significantly impaired FDC-B cell cluster formation and cell survival. In untreated cultures of intact clusters, cell proliferation showed a moderate reduction. In tissues, Cx43 protein levels run parallel with the density of FDC both in reactive germinal centers and in malformed follicles of follicular lymphomas and showed strong upregulation in newly generated and/or degrading bi-/multinuclear FDC of rudimentary processes. However, the inverse correlation between Cx43 expression and B cell proliferation seen in reactive germinal centers was not detected in follicular lymphomas. Furthermore, Cx43 levels were not associated with either lymphoma grade or bone marrow involvement. Our results suggest that Cx43 channels are critical in FDC and “ex vivo” germinal center development and in the persistence of FDC in follicular lymphomas but do not affect tumor progression. |
| format | Article |
| id | doaj-art-9775284888564a0aa1d0f2461de065b2 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9775284888564a0aa1d0f2461de065b22025-02-03T01:01:51ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/528098528098Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular LymphomasHajnalka Rajnai0Ivett Teleki1Gergo Kiszner2Nora Meggyesházi3Peter Balla4Tamas Vancsik5Gyorgyi Muzes6Judit Csomor7Andras Matolcsy8Tibor Krenacs91st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary2nd Department of Internal Medicine, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, Hungary1st Department of Pathology and Experimental Cancer Research, 1085 Budapest, HungaryFollicular dendritic cells (FDC) show homo- and heterocellular metabolic coupling through connexin 43 (Cx43) gap junctions and support B cell selection and maturation in germinal centers. In follicular lymphomas B cells escape apoptosis while FDC develop abnormally. Here we tested Cx43 channels in reactive FDC development and follicular lymphomas. In culture, the treatment of FDC-B cell clusters (resembling to “ex vivo” germinal centers) with Gap27 peptide, mimicking the 2nd extracellular loop of Cx43 protein, significantly impaired FDC-B cell cluster formation and cell survival. In untreated cultures of intact clusters, cell proliferation showed a moderate reduction. In tissues, Cx43 protein levels run parallel with the density of FDC both in reactive germinal centers and in malformed follicles of follicular lymphomas and showed strong upregulation in newly generated and/or degrading bi-/multinuclear FDC of rudimentary processes. However, the inverse correlation between Cx43 expression and B cell proliferation seen in reactive germinal centers was not detected in follicular lymphomas. Furthermore, Cx43 levels were not associated with either lymphoma grade or bone marrow involvement. Our results suggest that Cx43 channels are critical in FDC and “ex vivo” germinal center development and in the persistence of FDC in follicular lymphomas but do not affect tumor progression.http://dx.doi.org/10.1155/2015/528098 |
| spellingShingle | Hajnalka Rajnai Ivett Teleki Gergo Kiszner Nora Meggyesházi Peter Balla Tamas Vancsik Gyorgyi Muzes Judit Csomor Andras Matolcsy Tibor Krenacs Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas Journal of Immunology Research |
| title | Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas |
| title_full | Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas |
| title_fullStr | Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas |
| title_full_unstemmed | Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas |
| title_short | Connexin 43 Communication Channels in Follicular Dendritic Cell Development and in Follicular Lymphomas |
| title_sort | connexin 43 communication channels in follicular dendritic cell development and in follicular lymphomas |
| url | http://dx.doi.org/10.1155/2015/528098 |
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