Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma
Pheochromocytomas (PCC) are rare tumors that arise in chromaffin tissue of the adrenal gland. PCC are frequently inherited through predisposing mutations in genes such as the von Hippel-Lindau (VHL) tumor suppressor. VHL is part of the VHL elongin BC protein complex that also includes CUL2/5, TCEB1,...
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/546347 |
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| author | David A. Rowbotham Katey S. S. Enfield Victor D. Martinez Kelsie L. Thu Emily A. Vucic Greg L. Stewart Kevin L. Bennewith Wan L. Lam |
| author_facet | David A. Rowbotham Katey S. S. Enfield Victor D. Martinez Kelsie L. Thu Emily A. Vucic Greg L. Stewart Kevin L. Bennewith Wan L. Lam |
| author_sort | David A. Rowbotham |
| collection | DOAJ |
| description | Pheochromocytomas (PCC) are rare tumors that arise in chromaffin tissue of the adrenal gland. PCC are frequently inherited through predisposing mutations in genes such as the von Hippel-Lindau (VHL) tumor suppressor. VHL is part of the VHL elongin BC protein complex that also includes CUL2/5, TCEB1, TCEB2, and RBX1; in normoxic conditions this complex targets hypoxia-inducible factor 1 alpha (HIF1A) for degradation, thus preventing a hypoxic response. VHL inactivation by genetic mechanisms, such as mutation and loss of heterozygosity, inhibits HIF1A degradation, even in the presence of oxygen, and induces a pseudohypoxic response. However, the described <10% VHL mutation rate cannot account for the high frequency of hypoxic response observed. Indeed, little is known about genetic mechanisms disrupting other complex component genes. Here, we show that, in a panel of 171 PCC tumors, 59.6% harbored gene copy number loss (CNL) of at least one complex component. CNL significantly reduced gene expression and was associated with enrichment of gene targets controlled by HIF1. Interestingly, we show that VHL-related renal clear cell carcinoma harbored disruption of VHL alone. Our results indicate that VHL elongin BC protein complex components other than VHL could be important for PCC tumorigenesis and merit further investigation. |
| format | Article |
| id | doaj-art-975faea950bb4960a30d67d54c64bc83 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | International Journal of Endocrinology |
| spelling | doaj-art-975faea950bb4960a30d67d54c64bc832025-02-03T00:59:29ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/546347546347Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in PheochromocytomaDavid A. Rowbotham0Katey S. S. Enfield1Victor D. Martinez2Kelsie L. Thu3Emily A. Vucic4Greg L. Stewart5Kevin L. Bennewith6Wan L. Lam7Department of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaDepartment of Integrative Oncology, BC Cancer Agency, Vancouver, BC, CanadaPheochromocytomas (PCC) are rare tumors that arise in chromaffin tissue of the adrenal gland. PCC are frequently inherited through predisposing mutations in genes such as the von Hippel-Lindau (VHL) tumor suppressor. VHL is part of the VHL elongin BC protein complex that also includes CUL2/5, TCEB1, TCEB2, and RBX1; in normoxic conditions this complex targets hypoxia-inducible factor 1 alpha (HIF1A) for degradation, thus preventing a hypoxic response. VHL inactivation by genetic mechanisms, such as mutation and loss of heterozygosity, inhibits HIF1A degradation, even in the presence of oxygen, and induces a pseudohypoxic response. However, the described <10% VHL mutation rate cannot account for the high frequency of hypoxic response observed. Indeed, little is known about genetic mechanisms disrupting other complex component genes. Here, we show that, in a panel of 171 PCC tumors, 59.6% harbored gene copy number loss (CNL) of at least one complex component. CNL significantly reduced gene expression and was associated with enrichment of gene targets controlled by HIF1. Interestingly, we show that VHL-related renal clear cell carcinoma harbored disruption of VHL alone. Our results indicate that VHL elongin BC protein complex components other than VHL could be important for PCC tumorigenesis and merit further investigation.http://dx.doi.org/10.1155/2014/546347 |
| spellingShingle | David A. Rowbotham Katey S. S. Enfield Victor D. Martinez Kelsie L. Thu Emily A. Vucic Greg L. Stewart Kevin L. Bennewith Wan L. Lam Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma International Journal of Endocrinology |
| title | Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma |
| title_full | Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma |
| title_fullStr | Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma |
| title_full_unstemmed | Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma |
| title_short | Multiple Components of the VHL Tumor Suppressor Complex Are Frequently Affected by DNA Copy Number Loss in Pheochromocytoma |
| title_sort | multiple components of the vhl tumor suppressor complex are frequently affected by dna copy number loss in pheochromocytoma |
| url | http://dx.doi.org/10.1155/2014/546347 |
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