Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2022/6082140 |
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| author | Faria Mahjabeen Jieh-Juen Yu James P. Chambers Rishein Gupta Bernard P. Arulanandam |
| author_facet | Faria Mahjabeen Jieh-Juen Yu James P. Chambers Rishein Gupta Bernard P. Arulanandam |
| author_sort | Faria Mahjabeen |
| collection | DOAJ |
| description | Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased 46%, while INF-γ increased 44%, consistent with a negative regulatory role of fetuin-A in inflammation. Importantly, the observed increased IFN-γ production was abrogated in fetuin-A-deficient AHSG mice suggesting that IFN-γ induction following Cm infection is fetuin-A dependent. Assessment of expression of genes associated with inflammation revealed fetuin-A-dependent upregulation of TBX21 (a Th1 cell-specific transcription factor) in the lungs of Cm-infected WT mice that correlated with IFN-γ induction. Additionally, the effect of fetuin-A deficiency in mounting an adaptive immune response to Cm infection was demonstrated using a splenocyte recall assay. Although preliminary in nature, these findings are suggestive of fetuin-A involvement following Cm pulmonary infection and underscores the need to investigate further the role of fetuin-A in the immune response and the consequences of its gene deletion. |
| format | Article |
| id | doaj-art-97103e95f3e24be0b6d514befb3e0f85 |
| institution | Kabale University |
| issn | 1687-9198 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-97103e95f3e24be0b6d514befb3e0f852025-08-20T03:37:09ZengWileyInternational Journal of Microbiology1687-91982022-01-01202210.1155/2022/6082140Influence of Fetuin-A on Chlamydia muridarum Pulmonary InfectionFaria Mahjabeen0Jieh-Juen Yu1James P. Chambers2Rishein Gupta3Bernard P. Arulanandam4Department of Molecular Microbiology and ImmunologyDepartment of Molecular Microbiology and ImmunologyDepartment of Molecular Microbiology and ImmunologyDepartment of Molecular Microbiology and ImmunologyDepartment of Molecular Microbiology and ImmunologyFetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased 46%, while INF-γ increased 44%, consistent with a negative regulatory role of fetuin-A in inflammation. Importantly, the observed increased IFN-γ production was abrogated in fetuin-A-deficient AHSG mice suggesting that IFN-γ induction following Cm infection is fetuin-A dependent. Assessment of expression of genes associated with inflammation revealed fetuin-A-dependent upregulation of TBX21 (a Th1 cell-specific transcription factor) in the lungs of Cm-infected WT mice that correlated with IFN-γ induction. Additionally, the effect of fetuin-A deficiency in mounting an adaptive immune response to Cm infection was demonstrated using a splenocyte recall assay. Although preliminary in nature, these findings are suggestive of fetuin-A involvement following Cm pulmonary infection and underscores the need to investigate further the role of fetuin-A in the immune response and the consequences of its gene deletion.http://dx.doi.org/10.1155/2022/6082140 |
| spellingShingle | Faria Mahjabeen Jieh-Juen Yu James P. Chambers Rishein Gupta Bernard P. Arulanandam Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection International Journal of Microbiology |
| title | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
| title_full | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
| title_fullStr | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
| title_full_unstemmed | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
| title_short | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
| title_sort | influence of fetuin a on chlamydia muridarum pulmonary infection |
| url | http://dx.doi.org/10.1155/2022/6082140 |
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