Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides
Protein hydrolysates could be a natural and safer source of antioxidant peptides. The purpose of this study was to optimize the hydrolysis of Huso dauricus × Acipenser schrenckii sturgeon proteins using bromelain and purify antioxidant peptides from hydrolysate. The degree of hydrolysis of 18.69...
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Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
2022-03-01
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| Series: | Polish Journal of Food and Nutrition Sciences |
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| Online Access: | http://journal.pan.olsztyn.pl/Antioxidant-Activity-of-Hybrid-Sturgeon-Huso-dauricus-Acipenser-schrenckii-Protein,146317,0,2.html |
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| author | Anwar Noman Yuxia Wang Chao Zhang Sherif Abed |
| author_facet | Anwar Noman Yuxia Wang Chao Zhang Sherif Abed |
| author_sort | Anwar Noman |
| collection | DOAJ |
| description | Protein hydrolysates could be a natural and safer source of antioxidant peptides. The purpose of this study was to optimize the hydrolysis of Huso dauricus × Acipenser schrenckii sturgeon proteins using bromelain and purify antioxidant peptides from hydrolysate. The degree of hydrolysis of 18.69% was obtained under the optimal conditions and hydrolysate had 94.76% solubility, 902 nm particle size and high antioxidant activity. The IC 50 for DPPH • and ABTS •+ scavenging activity were 3.14 and 3.81 mg/mL, respectively. The fraction of hydrolysate with a molecular weight of <1 kDa exhibited the highest antiradical activity against DPPH • with IC 50 of 2.10 mg/mL. In turn, the IC 5 0 of the most active fraction after the Sephadex G-15 separation was 1.77 mg/mL. The reverse phase high performance liquid chromatography (RP-HPLC) was used to purify the peptides from this fraction. The peptide with histidine, leucine and glycine (MW of 0.2955 kDa) exhibited the highest antioxidant activity (IC 50 of 1.33 mg/mL). The obtained fractions and peptides with antioxidant activity could be used as natural substitutes for synthetic antioxidants, especially in food and pharmaceuticals. |
| format | Article |
| id | doaj-art-970aa117324b497b9fb48697ae2cd2ec |
| institution | Kabale University |
| issn | 2083-6007 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences |
| record_format | Article |
| series | Polish Journal of Food and Nutrition Sciences |
| spelling | doaj-art-970aa117324b497b9fb48697ae2cd2ec2025-02-02T14:24:09ZengInstitute of Animal Reproduction and Food Research of the Polish Academy of SciencesPolish Journal of Food and Nutrition Sciences2083-60072022-03-01721798910.31883/pjfns/146317146317Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified PeptidesAnwar Noman0https://orcid.org/0000-0002-1096-6277Yuxia Wang1Chao Zhang2Sherif Abed3https://orcid.org/0000-0002-1866-6367Key Laboratory of Fermentation Resource and Application in Sichuan Higher Education, Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, 644000, Sichuan, ChinaKey Laboratory of Fermentation Resource and Application in Sichuan Higher Education, Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, 644000, Sichuan, ChinaKey Laboratory of Fermentation Resource and Application in Sichuan Higher Education, Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, 644000, Sichuan, ChinaFood and Dairy Science and Technology Department, Faculty of Environmental Agricultural Science, Arish University, North Sinai, EgyptProtein hydrolysates could be a natural and safer source of antioxidant peptides. The purpose of this study was to optimize the hydrolysis of Huso dauricus × Acipenser schrenckii sturgeon proteins using bromelain and purify antioxidant peptides from hydrolysate. The degree of hydrolysis of 18.69% was obtained under the optimal conditions and hydrolysate had 94.76% solubility, 902 nm particle size and high antioxidant activity. The IC 50 for DPPH • and ABTS •+ scavenging activity were 3.14 and 3.81 mg/mL, respectively. The fraction of hydrolysate with a molecular weight of <1 kDa exhibited the highest antiradical activity against DPPH • with IC 50 of 2.10 mg/mL. In turn, the IC 5 0 of the most active fraction after the Sephadex G-15 separation was 1.77 mg/mL. The reverse phase high performance liquid chromatography (RP-HPLC) was used to purify the peptides from this fraction. The peptide with histidine, leucine and glycine (MW of 0.2955 kDa) exhibited the highest antioxidant activity (IC 50 of 1.33 mg/mL). The obtained fractions and peptides with antioxidant activity could be used as natural substitutes for synthetic antioxidants, especially in food and pharmaceuticals.http://journal.pan.olsztyn.pl/Antioxidant-Activity-of-Hybrid-Sturgeon-Huso-dauricus-Acipenser-schrenckii-Protein,146317,0,2.htmlhybrid sturgeonenzymatic hydrolysishydrolysis optimizationbromelaindegree of hydrolysisradical scavenging activitypeptide purification |
| spellingShingle | Anwar Noman Yuxia Wang Chao Zhang Sherif Abed Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides Polish Journal of Food and Nutrition Sciences hybrid sturgeon enzymatic hydrolysis hydrolysis optimization bromelain degree of hydrolysis radical scavenging activity peptide purification |
| title | Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides |
| title_full | Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides |
| title_fullStr | Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides |
| title_full_unstemmed | Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides |
| title_short | Antioxidant Activity of Hybrid Sturgeon ( Huso dauricus × Acipenser schrenckii ) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides |
| title_sort | antioxidant activity of hybrid sturgeon huso dauricus acipenser schrenckii protein hydrolysate prepared using bromelain its fractions and purified peptides |
| topic | hybrid sturgeon enzymatic hydrolysis hydrolysis optimization bromelain degree of hydrolysis radical scavenging activity peptide purification |
| url | http://journal.pan.olsztyn.pl/Antioxidant-Activity-of-Hybrid-Sturgeon-Huso-dauricus-Acipenser-schrenckii-Protein,146317,0,2.html |
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