Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Differe...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2002/393686 |
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| author | A. Elzagheid Y. Collan |
| author_facet | A. Elzagheid Y. Collan |
| author_sort | A. Elzagheid |
| collection | DOAJ |
| description | 48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Different sampling strategies were applied. Four methods were tested: method 1: cell groups measured, method 2: all cells measured, method 3: free cells measured, and method 4: atypical free cells measured. Method 4 showed most often DNA aneuploid histogram patterns, sampling method 1 had the highest number of DNA diploid histogram patterns. Diagnostic approaches may benefit from a sampling method detecting the hiding aneuploid cell population. Grading of neoplasm could potentially benefit from other approaches. |
| format | Article |
| id | doaj-art-970283e29cf84b0f8e8c758c2f7775d9 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-970283e29cf84b0f8e8c758c2f7775d92025-02-03T06:14:02ZengWileyAnalytical Cellular Pathology0921-89121878-36512002-01-01244-515916510.1155/2002/393686Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the BreastA. Elzagheid0Y. Collan1Department of Pathology, University of Turku, Kiinamyllynkatu 10, FIN‐20520, Turku, FinlandDepartment of Pathology, University of Turku, Kiinamyllynkatu 10, FIN‐20520, Turku, Finland48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Different sampling strategies were applied. Four methods were tested: method 1: cell groups measured, method 2: all cells measured, method 3: free cells measured, and method 4: atypical free cells measured. Method 4 showed most often DNA aneuploid histogram patterns, sampling method 1 had the highest number of DNA diploid histogram patterns. Diagnostic approaches may benefit from a sampling method detecting the hiding aneuploid cell population. Grading of neoplasm could potentially benefit from other approaches.http://dx.doi.org/10.1155/2002/393686 |
| spellingShingle | A. Elzagheid Y. Collan Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast Analytical Cellular Pathology |
| title | Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast |
| title_full | Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast |
| title_fullStr | Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast |
| title_full_unstemmed | Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast |
| title_short | Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast |
| title_sort | dependence of dna histograms on the sampling techniques in fine needle aspirates of the breast |
| url | http://dx.doi.org/10.1155/2002/393686 |
| work_keys_str_mv | AT aelzagheid dependenceofdnahistogramsonthesamplingtechniquesinfineneedleaspiratesofthebreast AT ycollan dependenceofdnahistogramsonthesamplingtechniquesinfineneedleaspiratesofthebreast |