Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS)
One of Maillard reaction products formed in the production of ammonia caramel is 4(5)-methylimidazole (4-MeI) classified as carcinogen. A method of 4-MeI determination based on ion-pair extraction and derivatisation with isobutyl chloroformate with subsequent gas chromatography-tandem mass spectrome...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2018/4696074 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555348571979776 |
|---|---|
| author | Martyna N. Wieczorek Krzysztof Przygoński Henryk H. Jeleń |
| author_facet | Martyna N. Wieczorek Krzysztof Przygoński Henryk H. Jeleń |
| author_sort | Martyna N. Wieczorek |
| collection | DOAJ |
| description | One of Maillard reaction products formed in the production of ammonia caramel is 4(5)-methylimidazole (4-MeI) classified as carcinogen. A method of 4-MeI determination based on ion-pair extraction and derivatisation with isobutyl chloroformate with subsequent gas chromatography-tandem mass spectrometry analysis was proposed. Tandem mass spectrometry was applied to reduce the influence of matrix and increase the selectivity and sensitivity of the method. Triple quadrupole GC-MS system was used for this study. The collision energies were optimized for MRM mode. The detection (LOD) and quantification limits (LOQ) of the elaborated method were 17 and 37.8 μg kg−1, respectively, repeatability was <15% RSD for analyzed caramel samples, and the recovery for 4-MeI was 101%. Comparison of MS/MS with SIM detection on the same instrument proved almost 30 times lower LODs achieved by tandem mass spectrometry compared to SIM. Described method can be routinely used for monitoring 4-MeI as a quality and safety marker in the production of ammonia caramel. |
| format | Article |
| id | doaj-art-96f47b4f931846bbbd4fda446ebf7206 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-96f47b4f931846bbbd4fda446ebf72062025-02-03T05:48:29ZengWileyJournal of Food Quality0146-94281745-45572018-01-01201810.1155/2018/46960744696074Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS)Martyna N. Wieczorek0Krzysztof Przygoński1Henryk H. Jeleń2Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandDepartment of Food Concentrates and Starch Products, Institute of Agricultural and Food Biotechnology, Starołęcka 40, 61-361 Poznań, PolandFaculty of Food Science and Nutrition, Poznań University of Life Sciences, Wojska Polskiego 31, 60-624 Poznań, PolandOne of Maillard reaction products formed in the production of ammonia caramel is 4(5)-methylimidazole (4-MeI) classified as carcinogen. A method of 4-MeI determination based on ion-pair extraction and derivatisation with isobutyl chloroformate with subsequent gas chromatography-tandem mass spectrometry analysis was proposed. Tandem mass spectrometry was applied to reduce the influence of matrix and increase the selectivity and sensitivity of the method. Triple quadrupole GC-MS system was used for this study. The collision energies were optimized for MRM mode. The detection (LOD) and quantification limits (LOQ) of the elaborated method were 17 and 37.8 μg kg−1, respectively, repeatability was <15% RSD for analyzed caramel samples, and the recovery for 4-MeI was 101%. Comparison of MS/MS with SIM detection on the same instrument proved almost 30 times lower LODs achieved by tandem mass spectrometry compared to SIM. Described method can be routinely used for monitoring 4-MeI as a quality and safety marker in the production of ammonia caramel.http://dx.doi.org/10.1155/2018/4696074 |
| spellingShingle | Martyna N. Wieczorek Krzysztof Przygoński Henryk H. Jeleń Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS) Journal of Food Quality |
| title | Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS) |
| title_full | Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS) |
| title_fullStr | Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS) |
| title_full_unstemmed | Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS) |
| title_short | Determination of 4-Methylimidazole in Ammonia Caramel Using Gas Chromatography–Tandem Mass Spectrometry (GC-MS/MS) |
| title_sort | determination of 4 methylimidazole in ammonia caramel using gas chromatography tandem mass spectrometry gc ms ms |
| url | http://dx.doi.org/10.1155/2018/4696074 |
| work_keys_str_mv | AT martynanwieczorek determinationof4methylimidazoleinammoniacaramelusinggaschromatographytandemmassspectrometrygcmsms AT krzysztofprzygonski determinationof4methylimidazoleinammoniacaramelusinggaschromatographytandemmassspectrometrygcmsms AT henrykhjelen determinationof4methylimidazoleinammoniacaramelusinggaschromatographytandemmassspectrometrygcmsms |