Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice
Background and Aim. Mice with alymphoplasia (aly/aly) mutation characterized by a lack of lymph nodes, Peyer's patches, and well-defined lymphoid follicles in the spleen were found. In this study, we used splenectomized aly/aly mice to elucidate the effects of secondary lymphoid organs in the d...
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| Language: | English |
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Wiley
2010-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2010/614890 |
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| author | Heng-Xiao Wang Shuang-Qin Yi Jun Li Hayato Terayama Munekazu Naito Shuichi Hirai Ning Qu Nozomi Yi Masahiro Itoh |
| author_facet | Heng-Xiao Wang Shuang-Qin Yi Jun Li Hayato Terayama Munekazu Naito Shuichi Hirai Ning Qu Nozomi Yi Masahiro Itoh |
| author_sort | Heng-Xiao Wang |
| collection | DOAJ |
| description | Background and Aim. Mice with alymphoplasia (aly/aly) mutation characterized by a lack of lymph nodes, Peyer's patches, and well-defined lymphoid follicles in the spleen were found. In this study, we used splenectomized aly/aly mice to elucidate the effects of secondary lymphoid organs in the development of aly/aly autoimmune pancreatitis. Methods. Forty-eight 10-week-old aly/aly mice were divided into two groups for splenectomy and sham operation. Histological and immunohistochemical analyses of the pancreas were performed at the ages of 20, 30, and 40 weeks old after operation, respectively.
Results. Our results showed that mononuclear cell infiltration was restricted to the interlobular connective tissues at the age of 20 weeks, and not increase obviously at the age of 30 and 40 weeks in splenectomized aly/aly mice. Furthermore, an apparent decrease in the expressions of CD4+ T, CD8+ T, and B cells was detected in the pancreatic tissues compared with sham aly/aly mice, however, no significant difference in macrophage expression between mice with and without a splenectomy.
Conclusions. Inflammation infiltration and development of the pancreatitis in aly/aly mice were suppressed effectively after splenectomy, which was, at least partly, correlated to inhibition of the infiltration of T and B cells in pancreatic tissues but not to macrophages. |
| format | Article |
| id | doaj-art-96e81ea554704a2caa8721f6cf5f87ba |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-96e81ea554704a2caa8721f6cf5f87ba2025-02-03T01:32:53ZengWileyClinical and Developmental Immunology1740-25221740-25302010-01-01201010.1155/2010/614890614890Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly MiceHeng-Xiao Wang0Shuang-Qin Yi1Jun Li2Hayato Terayama3Munekazu Naito4Shuichi Hirai5Ning Qu6Nozomi Yi7Masahiro Itoh8Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy and Neuroembryology, University of Kanazawa, Takara-machi 13-1, Kanazawa 920-8640, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanDepartment of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, JapanBackground and Aim. Mice with alymphoplasia (aly/aly) mutation characterized by a lack of lymph nodes, Peyer's patches, and well-defined lymphoid follicles in the spleen were found. In this study, we used splenectomized aly/aly mice to elucidate the effects of secondary lymphoid organs in the development of aly/aly autoimmune pancreatitis. Methods. Forty-eight 10-week-old aly/aly mice were divided into two groups for splenectomy and sham operation. Histological and immunohistochemical analyses of the pancreas were performed at the ages of 20, 30, and 40 weeks old after operation, respectively. Results. Our results showed that mononuclear cell infiltration was restricted to the interlobular connective tissues at the age of 20 weeks, and not increase obviously at the age of 30 and 40 weeks in splenectomized aly/aly mice. Furthermore, an apparent decrease in the expressions of CD4+ T, CD8+ T, and B cells was detected in the pancreatic tissues compared with sham aly/aly mice, however, no significant difference in macrophage expression between mice with and without a splenectomy. Conclusions. Inflammation infiltration and development of the pancreatitis in aly/aly mice were suppressed effectively after splenectomy, which was, at least partly, correlated to inhibition of the infiltration of T and B cells in pancreatic tissues but not to macrophages.http://dx.doi.org/10.1155/2010/614890 |
| spellingShingle | Heng-Xiao Wang Shuang-Qin Yi Jun Li Hayato Terayama Munekazu Naito Shuichi Hirai Ning Qu Nozomi Yi Masahiro Itoh Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice Clinical and Developmental Immunology |
| title | Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice |
| title_full | Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice |
| title_fullStr | Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice |
| title_full_unstemmed | Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice |
| title_short | Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice |
| title_sort | effects of splenectomy on spontaneously chronic pancreatitis in aly aly mice |
| url | http://dx.doi.org/10.1155/2010/614890 |
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