Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure
Anaerobic digestion (AD) technology offers significant advantages in addressing environmental issues arising from the intensification of livestock production since it enables waste reduction and energy recovery. However, the molecular composition of dissolved organic matter (DOM) and its linkages to...
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MDPI AG
2025-01-01
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| author | Juan Hu Yurui Zeng Aibin Hu Xiaofeng Wang |
| author_facet | Juan Hu Yurui Zeng Aibin Hu Xiaofeng Wang |
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| description | Anaerobic digestion (AD) technology offers significant advantages in addressing environmental issues arising from the intensification of livestock production since it enables waste reduction and energy recovery. However, the molecular composition of dissolved organic matter (DOM) and its linkages to microbial biodiversity during the industrial-scale AD process of chicken manure (CM) remains unclear. In this study, the chemical structure of CM digestate-derived DOM was characterized by using multi-spectroscopic techniques and ultrahigh-resolution mass spectrometry, and the microbial composition was detected by using 16S rRNA gene sequencing. The results revealed that the DOM contained abundant free amino acids and protein-like compounds but fewer humic-like substances, identified as lignin/carboxylate-rich alicyclic molecules, lipids, and proteins/aliphatic compounds featuring enriched S<sub>5–6</sub>O<sub>1</sub> and N<sub>1–5</sub>O<sub>X</sub> fragments. In addition, the 16S rRNA results revealed microorganisms that were centered on metabolic function in the production of volatile fatty acids, H<sub>2</sub>S/CH<sub>4</sub>, and the hydrolysis reaction in the AD process. Free amino acids and protein-like compounds were mainly associated with hydrolysis reactions and H<sub>2</sub>S production functional microorganisms. Lignin/carboxylate-rich alicyclic molecules were linked to microorganisms possessing hydrolysis reactions and, indirectly, CH<sub>4</sub> production. This study elucidates the linkage with the microbial and molecular composition of DOM, establishing a theoretical foundation for employing AD in the disposal of CM. |
| format | Article |
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| institution | Kabale University |
| issn | 2305-6304 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-96e80307610d43eeb423d6902c4c9aac2025-01-24T13:51:03ZengMDPI AGToxics2305-63042025-01-011314910.3390/toxics13010049Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken ManureJuan Hu0Yurui Zeng1Aibin Hu2Xiaofeng Wang3Collaborative Innovation Center for Emissions Trading System Co-Constructed by the Province and Ministry, Wuhan 430205, ChinaHubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang 438000, ChinaHubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Huanggang Normal University, Huanggang 438000, ChinaSchool of Computer Science and Hubei Key Laboratory of Intelligent Geo-Information Processing, China University of Geosciences, Wuhan 430078, ChinaAnaerobic digestion (AD) technology offers significant advantages in addressing environmental issues arising from the intensification of livestock production since it enables waste reduction and energy recovery. However, the molecular composition of dissolved organic matter (DOM) and its linkages to microbial biodiversity during the industrial-scale AD process of chicken manure (CM) remains unclear. In this study, the chemical structure of CM digestate-derived DOM was characterized by using multi-spectroscopic techniques and ultrahigh-resolution mass spectrometry, and the microbial composition was detected by using 16S rRNA gene sequencing. The results revealed that the DOM contained abundant free amino acids and protein-like compounds but fewer humic-like substances, identified as lignin/carboxylate-rich alicyclic molecules, lipids, and proteins/aliphatic compounds featuring enriched S<sub>5–6</sub>O<sub>1</sub> and N<sub>1–5</sub>O<sub>X</sub> fragments. In addition, the 16S rRNA results revealed microorganisms that were centered on metabolic function in the production of volatile fatty acids, H<sub>2</sub>S/CH<sub>4</sub>, and the hydrolysis reaction in the AD process. Free amino acids and protein-like compounds were mainly associated with hydrolysis reactions and H<sub>2</sub>S production functional microorganisms. Lignin/carboxylate-rich alicyclic molecules were linked to microorganisms possessing hydrolysis reactions and, indirectly, CH<sub>4</sub> production. This study elucidates the linkage with the microbial and molecular composition of DOM, establishing a theoretical foundation for employing AD in the disposal of CM.https://www.mdpi.com/2305-6304/13/1/49anaerobic digestionchicken manuredissolved organic mattermolecular characterizationmicrobial community structure |
| spellingShingle | Juan Hu Yurui Zeng Aibin Hu Xiaofeng Wang Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure Toxics anaerobic digestion chicken manure dissolved organic matter molecular characterization microbial community structure |
| title | Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure |
| title_full | Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure |
| title_fullStr | Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure |
| title_full_unstemmed | Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure |
| title_short | Exploring the Molecular Composition of Dissolved Organic Matter and Its Connection to Microbial Communities in Industrial-Scale Anaerobic Digestion of Chicken Manure |
| title_sort | exploring the molecular composition of dissolved organic matter and its connection to microbial communities in industrial scale anaerobic digestion of chicken manure |
| topic | anaerobic digestion chicken manure dissolved organic matter molecular characterization microbial community structure |
| url | https://www.mdpi.com/2305-6304/13/1/49 |
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