Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats
A rapid and sensitive quantitative analytical method was established for the simultaneous determination of five chromones (prim-O-glucosylcimifugin, cimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, 5-O-methylvisammiol, and sec-o-glucosylhamaudol) in the plasma of RS-treated rats for the first time...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/6454252 |
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| author | Li Meng Hui Gao Bin Chen Peng-peng Liu Guo-shun Shan Fan Zhang Tian-zhu Jia |
| author_facet | Li Meng Hui Gao Bin Chen Peng-peng Liu Guo-shun Shan Fan Zhang Tian-zhu Jia |
| author_sort | Li Meng |
| collection | DOAJ |
| description | A rapid and sensitive quantitative analytical method was established for the simultaneous determination of five chromones (prim-O-glucosylcimifugin, cimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, 5-O-methylvisammiol, and sec-o-glucosylhamaudol) in the plasma of RS-treated rats for the first time using ultra performance liquid chromatography- (UPLC-) tandem mass spectrometry. The Waters Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) was used as the chromatographic column, 0.1% formic acid water and 0.1% formic acid acetonitrile comprised the mobile phases, and all samples were determined under positive ion mode. The results showed that all analytes had good linearity (r>0.9902), between-day and within-day precisions less than 15%, accuracy between −5.50% and 5.53%, and extraction recovery between 88.26% and 97.65%. Both the matrix effect and stability met the requirements. This method was successfully applied for the comparative pharmacokinetics of five active components of RS in normal and febrile rats. The results showed that the pharmacokinetic behavior of RS extract significantly differed between the two types of rats. |
| format | Article |
| id | doaj-art-96e3d9ca97d84d8b9e36f089ffad62cb |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-96e3d9ca97d84d8b9e36f089ffad62cb2025-02-03T00:59:13ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732019-01-01201910.1155/2019/64542526454252Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile RatsLi Meng0Hui Gao1Bin Chen2Peng-peng Liu3Guo-shun Shan4Fan Zhang5Tian-zhu Jia6School of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaA rapid and sensitive quantitative analytical method was established for the simultaneous determination of five chromones (prim-O-glucosylcimifugin, cimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, 5-O-methylvisammiol, and sec-o-glucosylhamaudol) in the plasma of RS-treated rats for the first time using ultra performance liquid chromatography- (UPLC-) tandem mass spectrometry. The Waters Acquity UPLC BEH C18 (50 mm × 2.1 mm, 1.7 μm) was used as the chromatographic column, 0.1% formic acid water and 0.1% formic acid acetonitrile comprised the mobile phases, and all samples were determined under positive ion mode. The results showed that all analytes had good linearity (r>0.9902), between-day and within-day precisions less than 15%, accuracy between −5.50% and 5.53%, and extraction recovery between 88.26% and 97.65%. Both the matrix effect and stability met the requirements. This method was successfully applied for the comparative pharmacokinetics of five active components of RS in normal and febrile rats. The results showed that the pharmacokinetic behavior of RS extract significantly differed between the two types of rats.http://dx.doi.org/10.1155/2019/6454252 |
| spellingShingle | Li Meng Hui Gao Bin Chen Peng-peng Liu Guo-shun Shan Fan Zhang Tian-zhu Jia Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats Journal of Analytical Methods in Chemistry |
| title | Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats |
| title_full | Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats |
| title_fullStr | Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats |
| title_full_unstemmed | Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats |
| title_short | Simultaneous Determination of Five Chromones of Radix Saposhnikoviae Extract in Rat Plasma by UPLC-MS/MS: Application to a Comparative Pharmacokinetic Study in Normal and Febrile Rats |
| title_sort | simultaneous determination of five chromones of radix saposhnikoviae extract in rat plasma by uplc ms ms application to a comparative pharmacokinetic study in normal and febrile rats |
| url | http://dx.doi.org/10.1155/2019/6454252 |
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