Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4
Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, β-galactosidase and...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-03-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021001038 |
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| author | Pin Chen Xiaoqian Chen Wei Yu Bo Zhou Lihua Liu Yuzhuo Yang Peng Du Libo Liu Chun Li |
| author_facet | Pin Chen Xiaoqian Chen Wei Yu Bo Zhou Lihua Liu Yuzhuo Yang Peng Du Libo Liu Chun Li |
| author_sort | Pin Chen |
| collection | DOAJ |
| description | Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, β-galactosidase and Na+, K+-ATPase after 1/2 minimum bacteriostatic concentration (MIC) CIP treatment were significantly decreased (P < 0.01). Gas chromatography-mass spectrometry was used to analysis the changes of main metabolites in the cells and principal component analysis and partial least square model were constructed. The results indicated that CIP could cause changes in intracellular fatty acids, carbohydrates and amino acids, and the mechanism of amino acid metabolism under CIP stress was significantly inhibited. L. plantarum DNZ-4 made stress response to CIP by regulating the ratio of saturated fatty acids and unsaturated fats. This experiment revealed the changes of growth and metabolism mechanism of L. plantarum DNZ-4 under CIP stress, which help to provide technical means for the development of effective probiotics preparation products. |
| format | Article |
| id | doaj-art-96e156a78ebb48ecab15d5b736f56ccc |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-96e156a78ebb48ecab15d5b736f56ccc2025-02-03T10:39:14ZengTsinghua University PressFood Science and Human Wellness2213-45302022-03-01112332340Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4Pin Chen0Xiaoqian Chen1Wei Yu2Bo Zhou3Lihua Liu4Yuzhuo Yang5Peng Du6Libo Liu7Chun Li8Key Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, ChinaInstitute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaNational Dairy Product Quality Supervision and Inspection Center, Harbin 150024, ChinaKey Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.Key Laboratory of Dairy Science, Ministry of Education, Food Science College, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.Ciprofloxacin (CIP) is an antibiotic used to treat infections caused by bacteria. In this experiment, key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 was researched under CIP stress. The results showed that the activities of hexokinase, pyruvate kinase, β-galactosidase and Na+, K+-ATPase after 1/2 minimum bacteriostatic concentration (MIC) CIP treatment were significantly decreased (P < 0.01). Gas chromatography-mass spectrometry was used to analysis the changes of main metabolites in the cells and principal component analysis and partial least square model were constructed. The results indicated that CIP could cause changes in intracellular fatty acids, carbohydrates and amino acids, and the mechanism of amino acid metabolism under CIP stress was significantly inhibited. L. plantarum DNZ-4 made stress response to CIP by regulating the ratio of saturated fatty acids and unsaturated fats. This experiment revealed the changes of growth and metabolism mechanism of L. plantarum DNZ-4 under CIP stress, which help to provide technical means for the development of effective probiotics preparation products.http://www.sciencedirect.com/science/article/pii/S2213453021001038CiprofloxacinLactobacillus plantarum DNZ-4Key enzymesMetabolism |
| spellingShingle | Pin Chen Xiaoqian Chen Wei Yu Bo Zhou Lihua Liu Yuzhuo Yang Peng Du Libo Liu Chun Li Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 Food Science and Human Wellness Ciprofloxacin Lactobacillus plantarum DNZ-4 Key enzymes Metabolism |
| title | Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 |
| title_full | Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 |
| title_fullStr | Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 |
| title_full_unstemmed | Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 |
| title_short | Ciprofloxacin stress changes key enzymes and intracellular metabolites of Lactobacillus plantarum DNZ-4 |
| title_sort | ciprofloxacin stress changes key enzymes and intracellular metabolites of lactobacillus plantarum dnz 4 |
| topic | Ciprofloxacin Lactobacillus plantarum DNZ-4 Key enzymes Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021001038 |
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