Protocol to develop A 3D tumor model for drug testing applications
Three-dimensional (3D) tumor models provide physiologically relevant tumor environments and have become a major tool in cancer research and drug discovery. This article presents a protocol for creating a 3D organotypic tumor model by embedding a cancer cell spheroid within a collagen matrix containi...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | SLAS Discovery |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2472555224000686 |
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| _version_ | 1832590898717786112 |
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| author | Prasiddha Guragain Sunil Singh Hossein Tavana |
| author_facet | Prasiddha Guragain Sunil Singh Hossein Tavana |
| author_sort | Prasiddha Guragain |
| collection | DOAJ |
| description | Three-dimensional (3D) tumor models provide physiologically relevant tumor environments and have become a major tool in cancer research and drug discovery. This article presents a protocol for creating a 3D organotypic tumor model by embedding a cancer cell spheroid within a collagen matrix containing dispersed fibroblasts. This model offers significant advantages over the conventional monolayer cell culture, monoculture spheroids of cancer cells, and intermixed co-culture of cancer and stromal cells by mimicking the spatial organization and mechanical properties of a solid tumor. Compatible with robotic automation, our protocol significantly enhances reproducibility and scalability of creating a tumor model to study tumor-stromal interactions and test therapeutic compounds |
| format | Article |
| id | doaj-art-96cac8e20a2040118b3fe181c74e004b |
| institution | Kabale University |
| issn | 2472-5552 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | SLAS Discovery |
| spelling | doaj-art-96cac8e20a2040118b3fe181c74e004b2025-01-23T05:27:26ZengElsevierSLAS Discovery2472-55522025-01-0130100206Protocol to develop A 3D tumor model for drug testing applicationsPrasiddha Guragain0Sunil Singh1Hossein Tavana2The University of Akron, Akron, OH, 44325, USAThe University of Akron, Akron, OH, 44325, USACorresponding author.; The University of Akron, Akron, OH, 44325, USAThree-dimensional (3D) tumor models provide physiologically relevant tumor environments and have become a major tool in cancer research and drug discovery. This article presents a protocol for creating a 3D organotypic tumor model by embedding a cancer cell spheroid within a collagen matrix containing dispersed fibroblasts. This model offers significant advantages over the conventional monolayer cell culture, monoculture spheroids of cancer cells, and intermixed co-culture of cancer and stromal cells by mimicking the spatial organization and mechanical properties of a solid tumor. Compatible with robotic automation, our protocol significantly enhances reproducibility and scalability of creating a tumor model to study tumor-stromal interactions and test therapeutic compoundshttp://www.sciencedirect.com/science/article/pii/S2472555224000686Tumor modelCollagenSpheroidsFibroblastsOrganotypic microtissue |
| spellingShingle | Prasiddha Guragain Sunil Singh Hossein Tavana Protocol to develop A 3D tumor model for drug testing applications SLAS Discovery Tumor model Collagen Spheroids Fibroblasts Organotypic microtissue |
| title | Protocol to develop A 3D tumor model for drug testing applications |
| title_full | Protocol to develop A 3D tumor model for drug testing applications |
| title_fullStr | Protocol to develop A 3D tumor model for drug testing applications |
| title_full_unstemmed | Protocol to develop A 3D tumor model for drug testing applications |
| title_short | Protocol to develop A 3D tumor model for drug testing applications |
| title_sort | protocol to develop a 3d tumor model for drug testing applications |
| topic | Tumor model Collagen Spheroids Fibroblasts Organotypic microtissue |
| url | http://www.sciencedirect.com/science/article/pii/S2472555224000686 |
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