Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus
Epstein-Barr virus is a very common human virus that infects 90% of human adults. EBV replicates in epithelial and B cells and causes infectious mononucleosis. EBV infection is also linked to various cancers, including Burkitt’s lymphoma and nasopharyngeal carcinomas, and autoimmune diseases such as...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/9363750 |
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| _version_ | 1832568302583414784 |
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| author | Julio Alonso-Padilla Esther M. Lafuente Pedro A. Reche |
| author_facet | Julio Alonso-Padilla Esther M. Lafuente Pedro A. Reche |
| author_sort | Julio Alonso-Padilla |
| collection | DOAJ |
| description | Epstein-Barr virus is a very common human virus that infects 90% of human adults. EBV replicates in epithelial and B cells and causes infectious mononucleosis. EBV infection is also linked to various cancers, including Burkitt’s lymphoma and nasopharyngeal carcinomas, and autoimmune diseases such as multiple sclerosis. Currently, there are no effective drugs or vaccines to treat or prevent EBV infection. Herein, we applied a computer-aided strategy to design a prophylactic epitope vaccine ensemble from experimentally defined T and B cell epitopes. Such strategy relies on identifying conserved epitopes in conjunction with predictions of HLA presentation for T cell epitope selection and calculations of accessibility and flexibility for B cell epitope selection. The T cell component includes 14 CD8 T cell epitopes from early antigens and 4 CD4 T cell epitopes, targeted during the course of a natural infection and providing a population protection coverage of over 95% and 81.8%, respectively. The B cell component consists of 3 experimentally defined B cell epitopes from gp350 plus 4 predicted B cell epitopes from other EBV envelope glycoproteins, all mapping in flexible and solvent accessible regions. We discuss the rationale for the formulation and possible deployment of this epitope vaccine ensemble. |
| format | Article |
| id | doaj-art-96c108d08336437db3558865f6cc11ec |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-96c108d08336437db3558865f6cc11ec2025-02-03T00:59:18ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/93637509363750Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr VirusJulio Alonso-Padilla0Esther M. Lafuente1Pedro A. Reche2Barcelona Institute for Global Health (ISGlobal), Centre for Research in International Health (CRESIB), Hospital Clinic-University of Barcelona, Barcelona, SpainLaboratory of Immunomedicine, Faculty of Medicine, University Complutense of Madrid, Ave Complutense S/N, 28040 Madrid, SpainLaboratory of Immunomedicine, Faculty of Medicine, University Complutense of Madrid, Ave Complutense S/N, 28040 Madrid, SpainEpstein-Barr virus is a very common human virus that infects 90% of human adults. EBV replicates in epithelial and B cells and causes infectious mononucleosis. EBV infection is also linked to various cancers, including Burkitt’s lymphoma and nasopharyngeal carcinomas, and autoimmune diseases such as multiple sclerosis. Currently, there are no effective drugs or vaccines to treat or prevent EBV infection. Herein, we applied a computer-aided strategy to design a prophylactic epitope vaccine ensemble from experimentally defined T and B cell epitopes. Such strategy relies on identifying conserved epitopes in conjunction with predictions of HLA presentation for T cell epitope selection and calculations of accessibility and flexibility for B cell epitope selection. The T cell component includes 14 CD8 T cell epitopes from early antigens and 4 CD4 T cell epitopes, targeted during the course of a natural infection and providing a population protection coverage of over 95% and 81.8%, respectively. The B cell component consists of 3 experimentally defined B cell epitopes from gp350 plus 4 predicted B cell epitopes from other EBV envelope glycoproteins, all mapping in flexible and solvent accessible regions. We discuss the rationale for the formulation and possible deployment of this epitope vaccine ensemble.http://dx.doi.org/10.1155/2017/9363750 |
| spellingShingle | Julio Alonso-Padilla Esther M. Lafuente Pedro A. Reche Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus Journal of Immunology Research |
| title | Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus |
| title_full | Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus |
| title_fullStr | Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus |
| title_full_unstemmed | Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus |
| title_short | Computer-Aided Design of an Epitope-Based Vaccine against Epstein-Barr Virus |
| title_sort | computer aided design of an epitope based vaccine against epstein barr virus |
| url | http://dx.doi.org/10.1155/2017/9363750 |
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